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排序方式: 共有117条查询结果,搜索用时 15 毫秒
1.
Sung-Min Kim Heejaung Kim Jeong-Seon Lee Kyung Seok Park Gye Sun Jeon Jeeheun Shon Suk-Won Ahn Seung Hyun Kim Kyung Min Lee Jung-Joon Sung Kwang-Woo Lee 《PloS one》2013,8(11)
Background
Patients with ALS may be exposed to variable degrees of chronic intermittent hypoxia. However, all previous experimental studies on the effects of hypoxia in ALS have only used a sustained hypoxia model and it is possible that chronic intermittent hypoxia exerts effects via a different molecular mechanism from that of sustained hypoxia. No study has yet shown that hypoxia (either chronic intermittent or sustained) can affect the loss of motor neurons or cognitive function in an in vivo model of ALS.Objective
To evaluate the effects of chronic intermittent hypoxia on motor and cognitive function in ALS mice.Methods
Sixteen ALS mice and 16 wild-type mice were divided into 2 groups and subjected to either chronic intermittent hypoxia or normoxia for 2 weeks. The effects of chronic intermittent hypoxia on ALS mice were evaluated using the rotarod, Y-maze, and wire-hanging tests. In addition, numbers of motor neurons in the ventral horn of the spinal cord were counted and western blot analyses were performed for markers of oxidative stress and inflammatory pathway activation.Results
Compared to ALS mice kept in normoxic conditions, ALS mice that experienced chronic intermittent hypoxia had poorer motor learning on the rotarod test, poorer spatial memory on the Y-maze test, shorter wire hanging time, and fewer motor neurons in the ventral spinal cord. Compared to ALS-normoxic and wild-type mice, ALS mice that experienced chronic intermittent hypoxia had higher levels of oxidative stress and inflammation.Conclusions
Chronic intermittent hypoxia can aggravate motor neuronal death, neuromuscular weakness, and probably cognitive dysfunction in ALS mice. The generation of oxidative stress with activation of inflammatory pathways may be associated with this mechanism. Our study will provide insight into the association of hypoxia with disease progression, and in turn, the rationale for an early non-invasive ventilation treatment in patients with ALS. 相似文献2.
Miriam YH Ueda Paulo G Alvarenga Juliana M Real Eloisa de Sá Moreira Aripuan? Watanabe Ana Maria Passos-Castilho Matheus Vescovi Yana Novis Vanderson Rocha Adriana Seber Jose SR Oliveira Celso A Rodrigues Celso FH Granato 《Memórias do Instituto Oswaldo Cruz》2015,110(4):461-467
Human herpesvirus 6 (HHV-6) may cause severe complications after haematopoietic stem
cell transplantation (HSCT). Monitoring this virus and providing precise, rapid and
early diagnosis of related clinical diseases, constitute essential measures to
improve outcomes. A prospective survey on the incidence and clinical features of
HHV-6 infections after HSCT has not yet been conducted in Brazilian patients and the
impact of this infection on HSCT outcome remains unclear. A rapid test based on
real-time quantitative polymerase chain reaction (qPCR) has been optimised to screen
and quantify clinical samples for HHV-6. The detection step was based on reaction
with TaqMan® hydrolysis probes. A set of previously described primers and
probes have been tested to evaluate efficiency, sensitivity and reproducibility. The
target efficiency range was 91.4% with linearity ranging from 10-106
copies/reaction and a limit of detection of five copies/reaction or 250 copies/mL of
plasma. The qPCR assay developed in the present study was simple, rapid and
sensitive, allowing the detection of a wide range of HHV-6 loads. In conclusion, this
test may be useful as a practical tool to help elucidate the clinical relevance of
HHV-6 infection and reactivation in different scenarios and to determine the need for
surveillance. 相似文献
3.
Compartment‐specific aggregases direct distinct nuclear and cytoplasmic aggregate deposition 下载免费PDF全文
Annett Neuner Mohamed YH Mohamed D Lys Guilbride Karsten Richter Michael Lisby Elmar Schiebel Axel Mogk Bernd Bukau 《The EMBO journal》2015,34(6):778-797
Disruption of the functional protein balance in living cells activates protective quality control systems to repair damaged proteins or sequester potentially cytotoxic misfolded proteins into aggregates. The established model based on Saccharomyces cerevisiae indicates that aggregating proteins in the cytosol of eukaryotic cells partition between cytosolic juxtanuclear (JUNQ) and peripheral deposits. Substrate ubiquitination acts as the sorting principle determining JUNQ deposition and subsequent degradation. Here, we show that JUNQ unexpectedly resides inside the nucleus, defining a new intranuclear quality control compartment, INQ, for the deposition of both nuclear and cytosolic misfolded proteins, irrespective of ubiquitination. Deposition of misfolded cytosolic proteins at INQ involves chaperone‐assisted nuclear import via nuclear pores. The compartment‐specific aggregases, Btn2 (nuclear) and Hsp42 (cytosolic), direct protein deposition to nuclear INQ and cytosolic (CytoQ) sites, respectively. Intriguingly, Btn2 is transiently induced by both protein folding stress and DNA replication stress, with DNA surveillance proteins accumulating at INQ. Our data therefore reveal a bipartite, inter‐compartmental protein quality control system linked to DNA surveillance via INQ and Btn2. 相似文献
4.
5.
Yen-Chin Liu Alice YW Chang Yu-Chuan Tsai Julie YH Chan 《Journal of biomedical science》2009,16(1):8-14
Background
Both overproduction of nitric oxide (NO) and oxidative injury of cardiovascular and pulmonary systems contribute to fatal cardiovascular depression during endotoxemia. We investigated in the present study the relative contribution of oxidative stress and NO to cardiovascular depression during different stages of endotoxemia, and delineated their roles in cardiovascular protective effects of a commonly used anesthetic propofol during endotoxemia. 相似文献6.
Young-Seok Shon Hyung Y. Choi Michael S. Guerrero Chuhee Kwon 《Plasmonics (Norwell, Mass.)》2009,4(2):95-105
This article presents a concise review of preparation methods for transparent nanostructured films, with an emphasis on their
current applications in transmission-localized surface plasmon resonance (T-LSPR) sensing. One of the first methods used for
the fabrication of transparent nanostructured metal films is a direct vacuum evaporation of thin gold films. Self-induced
formations of small gold islands result in transparent nanostructured gold arrays. The most well-established method is a nanosphere
lithography developed by Van Duyne. Nanotriangular island arrays with controlled size and optical properties can be fabricated
by this protocol. A different nanolithography method known as focused ion beam milling is reported and used for the fabrication
of nanohole arrays. Simple assembly of solution-phase synthesized nanoparticles has also been utilized for the preparation
of nanoparticle arrays capable of T-LSPR sensing. Lastly, this article also describes a new preparation strategy, in which
self-assembly/thermolysis of nanoparticle multilayers is employed to obtain transparent nanoisland architectures on glass
substrates. 相似文献
7.
Effects of human amniotic membrane grafts combined with marrow mesenchymal stem cells on healing of full-thickness skin defects in rabbits 总被引:1,自引:0,他引:1
Sung Soo Kim Chang Keun Song Sung Keun Shon Kyu Yeol Lee Chul Hong Kim Myung Jin Lee Lih Wang 《Cell and tissue research》2009,336(1):59-66
We have investigated the wound-healing effects of mesenchymal stem cells (MSCs) in combination with human amniotic membrane
(HAM) when grafted into full-thickness skin defects of rabbits. Five defects in each of four groups were respectively treated
with HAM loaded with autologous MSCs (group A), HAM loaded with allologous MSCs (group B), HAM with injected autologous MSCs
(group C), and HAM with injected allologous MSCs (group D). The size of the wounds was calculated for each group at 7, 12,
and 15 days after grafting. The wounds were subsequently harvested at 25 days after grafting. Sections stained with hematoxylin
and eosin were used to determine the quality of wound healing, as based on the characteristics and amount of granulated tissue
in the epidermal and dermal layers. Groups A and B showed the most pronounced effect on wound closure, with statistically
significant improvement in wound healing being seen on post-operative days 7, 12, and 15. Although a slight trend toward improved
wound healing was seen in group A compared with group B, no statistically significant difference was found at any time point
between the two groups. Histological examination of healed wounds from groups A and B showed a thin epidermis with mature
differentiation and collagen bundle deposition plus recovered skin appendages in the dermal layer. In contrast, groups C and
D showed thickened epidermis with immature epithelial cells and increased fibroblast proliferation with only partially recovered
skin appendages in the dermal layer. Thus, the graft of HAM loaded with MSCs played an effective role during the healing of
skin defects in rabbits, with no significant difference being observed in wound healing between autologous and allologous
MSC transplantation.
This study was supported by research funds from Dong-A University. 相似文献
8.
Chunggab Choi Hye Min Kim Jeeheun Shon Jiae Park Hyeong-Taek Kim Suk Ho Kang Seung-Hun Oh Nam Keun Kim Ok Joon Kim 《Cytotherapy》2018,20(6):820-829
Background
The blood-brain barrier (BBB) presents a significant challenge to the therapeutic efficacy of stem cells in chronic stroke. Various methods have been developed to increase BBB permeability, but these are associated with adverse effects and are, therefore, not clinically applicable. We recently identified that combination drug treatment of mannitol and temozolomide improved BBB permeability in vitro. Here, we investigated whether this combination could increase the effectiveness of stem cell treatment in an animal model of chronic ischemic stroke.Methods
Chronic stroke was induced in rats by middle cerebral artery occlusion (MCAo). After then, rats were administered human umbilical cord–derived mesenchymal stromal cells (hUC-MSCs) by intravenous injection with or without combination drug treatment of mannitol and temozolomide. To evaluate the therapeutic efficacy, behavioral and immunohistochemical tests were performed, and the differences among control, stem cell only, combination drug only and stem cell with combination drug treatment were analyzed.Results
Although no hUC-MSCs were detected in any group, treatment with stem cells and combination drug of mannitol and temozolomide increased the intracerebral delivery of hCD63-positive microvesicles compared with stem cell only treatment. Furthermore, treatment with stem cells and drug combination ameliorated behavioral deficits and increased bromodeoxyuridine-, doublecortin- and Reca-1–positive cells in the perilesional area as compared with other groups.Discussion
The combination drug treatment of mannitol and temozolomide allowed for the efficient delivery of hUC-MSC–derived microvesicles into the brain in a chronic stroke rat model. This attenuated behavioral deficits, likely by improving neural regeneration and angiogenesis. Thus, combination drug treatment of mannitol and temozolomide could be a novel therapeutic option for patients with chronic ischemic stroke. 相似文献9.
10.
Microbial community analysis of an aerobic nitrifying-denitrifying MBR treating ABS resin wastewater 总被引:1,自引:0,他引:1
A two-stage aerobic membrane bioreactor (MBR) system for treating acrylonitrile butadiene styrene (ABS) resin wastewater was carried out in this study to evaluate the system performance on nitrification. The results showed that nitrification of the aerobic MBR system was significant and the highest TKN removal of approximately 90% was obtained at hydraulic retention time (HRT) 18 h. In addition, the result of nitrogen mass balance revealed that the percentage of TN removal due to denitrification was in the range of 8.7-19.8%. Microbial community analysis based on 16s rDNA molecular approach indicated that the dominant ammonia oxidizing bacteria (AOB) group in the system was a β-class ammonia oxidizer which was identified as uncultured sludge bacterium (AF234732). A heterotrophic aerobic denitrifier identified as Thauera mechernichensis was found in the system. The results indicated that a sole aerobic MBR system for simultaneous removals of carbon and nitrogen can be designed and operated for neglect with an anaerobic unit. 相似文献