全文获取类型
收费全文 | 3558篇 |
免费 | 234篇 |
国内免费 | 1篇 |
出版年
2022年 | 8篇 |
2021年 | 36篇 |
2020年 | 19篇 |
2019年 | 37篇 |
2018年 | 59篇 |
2017年 | 44篇 |
2016年 | 62篇 |
2015年 | 104篇 |
2014年 | 130篇 |
2013年 | 246篇 |
2012年 | 200篇 |
2011年 | 242篇 |
2010年 | 130篇 |
2009年 | 132篇 |
2008年 | 228篇 |
2007年 | 214篇 |
2006年 | 215篇 |
2005年 | 192篇 |
2004年 | 206篇 |
2003年 | 197篇 |
2002年 | 214篇 |
2001年 | 58篇 |
2000年 | 68篇 |
1999年 | 63篇 |
1998年 | 39篇 |
1997年 | 37篇 |
1996年 | 22篇 |
1995年 | 23篇 |
1994年 | 31篇 |
1993年 | 30篇 |
1992年 | 56篇 |
1991年 | 38篇 |
1990年 | 42篇 |
1989年 | 41篇 |
1988年 | 38篇 |
1987年 | 35篇 |
1986年 | 29篇 |
1985年 | 23篇 |
1984年 | 21篇 |
1983年 | 12篇 |
1982年 | 20篇 |
1981年 | 16篇 |
1980年 | 19篇 |
1979年 | 25篇 |
1978年 | 9篇 |
1977年 | 14篇 |
1976年 | 9篇 |
1975年 | 8篇 |
1974年 | 9篇 |
1969年 | 11篇 |
排序方式: 共有3793条查询结果,搜索用时 348 毫秒
1.
Kenji Tsuji Shinji KitamuraHirofumi Makino 《Biochemical and biophysical research communications》2014
The kidneys are exposed to hypoxic conditions during development. Hypoxia-inducible factor (HIF), an important mediator of the response to hypoxia, is believed to have an important role in development. However, the relationship between HIF and branching morphogenesis has not been elucidated clearly. 相似文献
2.
Shinji Hosoi Hiromasa Miyaji Mitsuo Satoh Tsukasa Kurimoto Akira Mihara Nobuo Fujiyoshi Seiga Itoh Seiji Sato 《Cytotechnology》1991,5(2):17-34
We investigated the basic technology of cell culture conditions for production of useful substances such as cytokines, and related proteins produced by Namalwa cells. Namalwa cells (Klein, 1972), human B lymphoblastoid cells, were used for large scale production of alpha-interferon (Klein, 1979). Namalwa KJM-1, a subline of Namalwa cells, adapted to serum- and albumin-free medium, can grow at a high density above 1 × 107 cells/ml in suspension mode by the use of a perfusion culture system, Biofermenter?, containing a cone-type cell-sedimentation column as cell separator (Sato, 1983).Several kinds of cytokine cDNA can be introduced and expressed in Namalwa KJM-1 cells (Miyaji, 1990a,b,c). Some of these were produced in large quantities by use of a gene amplification method with dhfr (Miyaji, 1990c), even though the Namalwa KJM-1 cells contained endogenous dhfr genes. For stable production of the target protein, Namalwa KJM-1 cells are very useful host cells, because they have no effective endogenous protease activity in the conditioned medium.Using Biofermenter with micro-silicone fibers and a dialysis system, the specific productivity of the target proteins was not depressed at a high cell density. 相似文献
3.
4.
Monoclonal antibodies against chick embryonic beta-galactoside-binding lectin were obtained. One of the monoclonal antibodies was ineffective in Western blotting and seemed to be unable to bind the SDS-denatured lectin. When the native lectin was dotted on a nitrocellulose filter and subjected to denaturation by treatment with SDS, urea or heat, binding of this antibody no longer occurred, though other monoclonal antibodies bound normally. This antibody seems to have been raised against an epitope which is destroyed upon denaturation. 相似文献
5.
Masanori Kasai Sadao Kiyohara 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2010,196(12):901-912
Feeding and locomotor activities of the Japanese catfish Plotosus japonicus under solitary condition were recorded to identify mechanisms controlling these behaviours. In the absence of food, the catfish
showed nocturnal locomotor activity, but no feeding activity. Under ad libitum food conditions, both feeding and locomotor
activities occurred during the dark period and were synchronized with light/dark (LD) cycles. Feeding activity lasted for
11–24 days when food was stopped after ad libitum food availability. Restricted food during the light phase produced both
food-anticipatory and light-entrainable feeding activity. Furthermore, this condition produced weak food-anticipatory and
light-entrainable locomotor activity. Under the light/light (LL) condition, restricted food produced food-anticipatory feeding
and locomotor activities, suggesting that a food-entrainable oscillator controls both feeding and locomotor activities. However,
under the LL condition, light-entrainable feeding and locomotor activities were not observed, suggesting that a light-entrainable
oscillator controls both feeding and locomotor activities. During a restricted food schedule, LD cycle shifts resulted in
disrupted synchronization of feeding activity onset in three of the four fish, but one fish showed synchronized feeding activity.
These results suggest that the food- and the light-entrainable oscillator may control feeding and locomotor activities, respectively. 相似文献
6.
7.
8.
The NSm nonstructural protein of Rift Valley fever virus (family Bunyaviridae, genus Phlebovirus) has an antiapoptotic function and affects viral pathogenesis. We found that NSm integrates into the mitochondrial outer membrane and that the protein''s N terminus is exposed to the cytoplasm. The C-terminal region of NSm, which contains a basic amino acid cluster and a putative transmembrane domain, targeted the protein to the mitochondrial outer membrane and exerted antiapoptotic function. 相似文献
9.
10.
H Kasai Z Yamaizumi F Yamamoto T Bessho S Nishimura M Berger J Cadet 《Nucleic acids symposium series》1992,(27):181-182
Potosensitized formation of 8-hydroxyguanine in DNA by riboflavin was observed. A reaction mechanism involving guanine radical cation and hydration reaction was proposed. This hypothesis was confirmed by the incorporation of [18O]-atom within guanine moiety in isotopic experiments using [18O]-H2O. Photosensitized formation of oh8Gua by riboflavin was also observed in cellular DNA. 相似文献