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Yoshinari Maeda Kiyoshi Yoshimura Hiroto Matsui Yoshitaro Shindo Takao Tamesa Yukio Tokumitsu Noriaki Hashimoto Yoshihiro Tokuhisa Kazuhiko Sakamoto Kouhei Sakai Yutaka Suehiro Yuji Hinoda Koji Tamada Shigefumi Yoshino Shoichi Hazama Masaaki Oka 《Cancer immunology, immunotherapy : CII》2015,64(8):1047-1056
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Monoclonal antibodies against chick embryonic beta-galactoside-binding lectin were obtained. One of the monoclonal antibodies was ineffective in Western blotting and seemed to be unable to bind the SDS-denatured lectin. When the native lectin was dotted on a nitrocellulose filter and subjected to denaturation by treatment with SDS, urea or heat, binding of this antibody no longer occurred, though other monoclonal antibodies bound normally. This antibody seems to have been raised against an epitope which is destroyed upon denaturation. 相似文献
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The alteration of hepatic drug-metabolizing enzyme activities in mice given Salmonella endotoxin by single or multiple intraperitoneal injections was investigated. An essentially the same biphasic, early and late phase, endotoxin tolerance was observed in the animals receiving a single injection of endotoxin or repetitive daily injections. The results of reciprocal cross tolerance tests using lipopolysaccharide and free lipid A preparations derived from Salmonella minnesota, Salmonella typhimurium, E. coli, Pseudomonas aeruginosa, and Chromobacterium violaceum suggested that lipid A moiety plays an important role in the induction of early endotoxin tolerance to endotoxin response. 相似文献
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Masanori Kasai Sadao Kiyohara 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2010,196(12):901-912
Feeding and locomotor activities of the Japanese catfish Plotosus japonicus under solitary condition were recorded to identify mechanisms controlling these behaviours. In the absence of food, the catfish
showed nocturnal locomotor activity, but no feeding activity. Under ad libitum food conditions, both feeding and locomotor
activities occurred during the dark period and were synchronized with light/dark (LD) cycles. Feeding activity lasted for
11–24 days when food was stopped after ad libitum food availability. Restricted food during the light phase produced both
food-anticipatory and light-entrainable feeding activity. Furthermore, this condition produced weak food-anticipatory and
light-entrainable locomotor activity. Under the light/light (LL) condition, restricted food produced food-anticipatory feeding
and locomotor activities, suggesting that a food-entrainable oscillator controls both feeding and locomotor activities. However,
under the LL condition, light-entrainable feeding and locomotor activities were not observed, suggesting that a light-entrainable
oscillator controls both feeding and locomotor activities. During a restricted food schedule, LD cycle shifts resulted in
disrupted synchronization of feeding activity onset in three of the four fish, but one fish showed synchronized feeding activity.
These results suggest that the food- and the light-entrainable oscillator may control feeding and locomotor activities, respectively. 相似文献
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Affinophoresis is an electrophoretic separation technique for biomolecules which uses an affinophore. An affinophore is a macromolecular polyelectrolyte bearing affinity ligands. It migrates rapidly in an electric field, and consequently the electrophoretic mobility of molecules having affinity for the ligand is specifically changed. This technique has now been incorporated in two-dimensional agarose gel electrophoresis in a procedure which utilizes normal electrophoresis in the first dimension and affinophoresis in the second dimension. Proteins which do not have affinity for the ligand migrate to locations along a diagonal line passing through the origin, whereas proteins which have affinity are carried away from the line by the affinophore. Accordingly, molecules having affinity for the ligand can be readily assigned. Trypsins contained in Pronase and pancreatin were separated by this procedure using an affinophore bearing a competitive inhibitor for trypsin, benzamidine, on a polyanionic molecule (a polyacrylic acid derivative). 相似文献
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Satoru Arata Tohru Hirayama Yuri Akiyama Jun-ichi Mashimo Nobuhiko Kasai Katsuhiko Yoshida Yoshiko Nakamura Yoshio Aoki 《FEMS microbiology letters》1989,60(2):223-225
Abstract In vitro antigenic reactivity of lipid A from Pseudomonas diminuta and Pseudomonas vesicularis with homologous and heterologous lipid A antibodies including monoclonal antibodies was studied by inhibition test of enzyme-linked immunosorbent assay (ELISA). The results suggest that both Pseudomonas lipid As have very similar epitopes, including species-specific and cross-reactive epitopes as compared with enterobacterial lipid A. 相似文献
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