全文获取类型
收费全文 | 730篇 |
免费 | 67篇 |
出版年
2022年 | 1篇 |
2021年 | 12篇 |
2020年 | 7篇 |
2019年 | 10篇 |
2018年 | 16篇 |
2017年 | 19篇 |
2016年 | 19篇 |
2015年 | 37篇 |
2014年 | 45篇 |
2013年 | 60篇 |
2012年 | 60篇 |
2011年 | 60篇 |
2010年 | 33篇 |
2009年 | 27篇 |
2008年 | 42篇 |
2007年 | 42篇 |
2006年 | 41篇 |
2005年 | 54篇 |
2004年 | 35篇 |
2003年 | 39篇 |
2002年 | 33篇 |
2001年 | 6篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1998年 | 8篇 |
1997年 | 8篇 |
1996年 | 9篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 9篇 |
1991年 | 3篇 |
1990年 | 8篇 |
1989年 | 6篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1983年 | 1篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1976年 | 2篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1953年 | 1篇 |
排序方式: 共有797条查询结果,搜索用时 15 毫秒
1.
Adriana Calderaro Giovanna Piccolo Chiara Gorrini Sara Montecchini Sabina Rossi Maria Cristina Medici Carlo Chezzi Georges Snounou 《PloS one》2012,7(10)
It has been proposed that ovale malaria in humans is caused by two closely related but distinct species of malaria parasites: P. ovale curtisi and P. ovale wallikeri. We have extended and optimized a Real-time PCR assay targeting the parasite’s small subunit ribosomal RNA (ssrRNA) gene to detect both these species. When the assay was applied to 31 archival blood samples from patients diagnosed with P. ovale, it was found that the infection in 20 was due to P. ovale curtisi and in the remaining 11 to P. ovale wallikeri. Thus, this assay provides a useful tool that can be applied to epidemiological investigations of the two newly recognized distinct P. ovale species, that might reveal if these species also differ in their clinical manifestation, drugs susceptibility and relapse periodicity. The results presented confirm that P. ovale wallikeri is not confined to Southeast Asia, since the majority of the patients analyzed in this study had acquired their P. ovale infection in African countries, mostly situated in West Africa. 相似文献
2.
3.
4.
Abstract: The subunit structure of brain glutamate decarboxylase in cerebellum was investigated by using gel electrophoresis and antisera that specifically recognize the individual isoforms of brain glutamate decarboxylase (termed GAD65 and GAD67 ). The antisera were prepared against peptides that corresponded to amino acid sequences specific to each isoform. Each antiserum reacted specifically with the appropriate peptide in an ELISA and with the appropriate form of GAD on immunoblots. Nondenaturing gradient gel electrophoresis indicated that GAD is principally multimeric with monomeric forms comprising <3% of the total. Immunoprecipitation and immunoaffinity chromatography experiments were performed with antisera W624 and W883, which were prepared against peptides specific to GAD65 and GAD67 , respectively. Immunoprecipitates prepared from cerebellar supernatants with W624 contained both GAD65 and GAD67 , whereas some GAD67 was left in the supernatant. In a similar manner, immunoprecipitates prepared with W883 contained both GAD65 and GAD67 , whereas some GAD65 remained in the supernatant. In addition, immunoaffinity columns prepared with either W624 or W883 retained both GAD65 and GAD67 even after extensive washing. These results are consistent with the presence of heteromultimers of GAD65 and GAD67 in cerebellum in addition to homomers of each form. 相似文献
5.
6.
S. Kleinle U. Wiesmann A. Superti-Furga S. Krähenbühl E. Boltshauser J. Reichen S. Liechti-Gallati 《Human genetics》1997,100(5-6):643-650
We used a strategy based on long PCR (polymerase chain reaction) for detection and characterization of mitochondrial DNA (mtDNA)
rearrangements in two patients with clinical signs suggesting Pearson syndrome and Kearns-Sayre syndrome (KSS), respectively,
and one patient with myopathic symptoms of unidentified origin. Mitochondrial DNA rearrangements were detected by amplification
of the complete mitochondrial genome (16.6 kb) using long PCR with primers located in essential regions of the mitochondrial
genome and quantified by three-primer PCR. Long PCR with deletion-specific primers was used for identification and quantitative
estimation of the different forms of rearranged molecules, such as deletions and duplications. We detected significant amounts
of a common 7.4-kb deletion flanked by a 12-bp direct repeat in all tissues tested from the patient with Pearson syndrome.
In skeletal muscle from the patient with clinical signs of KSS we found significant amounts of a novel 3.7-kb rearrangement
flanked by a 4-bp inverted repeat that was present in the form of deletions as well as duplications. In the patient suffering
from myopathic symptoms of unidentified origin we did not detect rearranged mtDNA in blood but found low levels of two rearranged
mtDNA populations in skeletal muscle, a previously described 7-kb deletion flanked by a 7-bp direct repeat and a novel 6.6-kb
deletion with no repeat. These two populations, however, were unlikely to be the cause of the myopathic symptoms as they were
present at low levels (10–40 ppm). Using a strategy based on screening with long PCR we were able to detect and characterize
high as well as low levels of mtDNA rearrangements in three patients.
Received: 10 March 1997 / Accepted: 20 May 1997 相似文献
7.
Sabina Iluta Sergiu Pasca Grigore Gafencu Ancuta Jurj Andreea Terec Patric Teodorescu Cristina Selicean Ciprian Jitaru Alexandra Preda Diana Cenariu Catalin Constantinescu Maria Iordache Bogdan Tigu Raluca Munteanu Richard Feder Delia Dima Mihnea Zdrenghea Diana Gulei Tudor-Eliade Ciuleanu Ciprian Tomuleasa 《Journal of cellular and molecular medicine》2021,25(13):6094-6102
8.
Vijayaganapathy Vaithilingam Cherry Fung Sabina Ratnapala Jayne Foster Vijesh Vaghjiani Ursula Manuelpillai Bernard E. Tuch 《PloS one》2013,8(3)
Xenotransplantation of microencapsulated fetal pig islet-like cell clusters (FP ICCs) offers a potential cellular therapy for type 1 diabetes. Although microcapsules prevent direct contact of the host immune system with the xenografted tissue, poor graft survival is still an issue. This study aimed to characterise the nature of the host immune cells present on the engrafted microcapsules and effects on encapsulated FP ICCs that were transplanted into immunocompetent mice. Encapsulated FP ICCs were transplanted into the peritoneal cavity of C57BL/6 mice. Grafts retrieved at days 1, 3, 7, 14 and 21 post-transplantation were analysed for pericapsular fibrotic overgrowth (PFO), cell viability, intragraft porcine gene expression, macrophages, myofibroblasts and intraperitoneal murine cytokines. Graft function was assessed ex vivo by insulin secretion studies. Xenogeneic immune response to encapsulated FP ICCs was associated with enhanced intragraft mRNA expression of porcine antigens MIP-1α, IL-8, HMGB1 and HSP90 seen within the first two weeks post-transplantation. This was associated with the recruitment of host macrophages, infiltration of myofibroblasts and collagen deposition leading to PFO which was evident from day 7 post-transplantation. This was accompanied by a decrease in cell viability and loss of FP ICC architecture. The only pro-inflammatory cytokine detected in the murine peritoneal flushing was TNF-α with levels peaking at day 7 post transplantation. This correlated with the onset of PFO at day 7 implying activated macrophages as its source. The anti-inflammatory cytokines detected were IL-5 and IL-4 with levels peaking at days 1 and 7, respectively. Porcine C-peptide was undetectable at all time points post-transplantation. PFO was absent and murine intraperitoneal cytokines were undetectable when empty microcapsules were transplanted. In conclusion, this study demonstrated that the macrophages are direct effectors of the xenogeneic immune response to encapsulated FP ICCs leading to PFO mediated by a combination of both pro- and anti-inflammatory cytokines. 相似文献
9.
P E Mullis S Liechti-Gallati L Di Silvio C G Brook A F Paes-Alves 《Hormone research》1991,36(1-2):4-8
A recent longitudinal study in children with cystic fibrosis (CF) challenged the common idea that CF is causing short stature. The data, however, showed clearly that short stature cannot be explained by CF alone after the first year of life. We report on a girl suffering from CF and short stature in whom DNA analysis using polymerase chain reaction and Southern blot techniques of the human growth hormone (hGH) gene cluster revealed a 6.7-kb gene deletion encompassing the hGH-1 gene. Anti-hGH antibodies of polyclonal origin developed, leading to a growth arrest after only 2 months of hGH replacement. In addition, a family study was performed, and the haplotypes of the CF gene and hGH gene cluster were analyzed. 相似文献
10.
Summary A family of an isolated patient with Becker muscular dystrophy has been investigated by DNA analysis. Southern blotting and
hybridization were performed with six probes (C7, pERT87.15, pERT87.1, pXJ1.1, pXJ2.3, 754) mapping in the Xp21 region. A
deletion within the pXJ region was demonstrated in the proband, his mother and all three sisters. The segregation pattern
for the restriction fragment length polymorphisms (RFLPs) observed with the pXJ probes as well as with pERT87.15, pERT87.1
and 754 probes indicates that the deletion is of grandpaternal origin. 相似文献