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1.
Robert Blake II Elizabeth A. Shute James Waskovsky Arthur P. Harrison JR. 《Geomicrobiology journal》2013,30(3-4):173-192
Abstract Microorganisms capable of aerobic respiration on ferrous ions are spread throughout eubacterial and archaebacterial phyla. Phylogenetically distinct organisms were shown to express spectrally distinct redox‐active biomolecules during autotrophic growth on soluble iron. A new iron‐oxidizing eubacterium, designated as strain Funis, was investigated. Strain Funis was judged to be different from other known iron‐oxidizing bacteria on the bases of comparative lipid analyses, 16S rRNA sequence analyses, and cytochrome composition studies. When grown autotrophically on ferrous ions, Funis produced conspicuous levels of a novel acid‐stable, acid‐soluble yellow cytochrome with a distinctive absorbance peak at 579 nm in the reduced state. Stopped‐flow spectrophotometric kinetic studies were conducted on respiratory chain components isolated from cell‐free extracts of Thiobacillus ferrooxidans. Experimental results were consistent with a model where the primary oxidant of ferrous ions is a highly aggregated c‐type cytochrome that then reduces the periplasmic rusticyanin. The Fe(II)‐dependent, cytochrome c‐catalyzed reduction of the rusticyanin possessed three kinetic properties in common with corresponding intact cells that respire on iron: the same anion specificity, a similar dependence of the rate on the concentration of ferrous ions, and similar rates at saturating concentrations of ferrous ions 相似文献
2.
Noriko Fujimoto Meikun Kan-o Tomoki Ushijima Yohko Kage Ryuji Tominaga Hideki Sumimoto Ryu Takeya 《PloS one》2016,11(2)
Fhod3 is a cardiac member of the formin family proteins that play pivotal roles in actin filament assembly in various cellular contexts. The targeted deletion of mouse Fhod3 gene leads to defects in cardiogenesis, particularly during myofibrillogenesis, followed by lethality at embryonic day (E) 11.5. However, it remains largely unknown how Fhod3 functions during myofibrillogenesis. In this study, to assess the mechanism whereby Fhod3 regulates myofibrillogenesis during embryonic cardiogenesis, we generated transgenic mice expressing Fhod3 selectively in embryonic cardiomyocytes under the control of the β-myosin heavy chain (MHC) promoter. Mice expressing wild-type Fhod3 in embryonic cardiomyocytes survive to adulthood and are fertile, whereas those expressing Fhod3 (I1127A) defective in binding to actin die by E11.5 with cardiac defects. This cardiac phenotype of the Fhod3 mutant embryos is almost identical to that observed in Fhod3 null embryos, suggesting that the actin-binding activity of Fhod3 is crucial for embryonic cardiogenesis. On the other hand, the β-MHC promoter-driven expression of wild-type Fhod3 sufficiently rescues cardiac defects of Fhod3-null embryos, indicating that the Fhod3 protein expressed in a transgenic manner can function properly to achieve myofibril maturation in embryonic cardiomyocytes. Using the transgenic mice, we further examined detailed localization of Fhod3 during myofibrillogenesis in situ and found that Fhod3 localizes to the specific central region of nascent sarcomeres prior to massive rearrangement of actin filaments and remains there throughout myofibrillogenesis. Taken together, the present findings suggest that, during embryonic cardiogenesis, Fhod3 functions as the essential reorganizer of actin filaments at the central region of maturating sarcomeres via the actin-binding activity of the FH2 domain. 相似文献
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KHALIFAH R. A.; LEWIS L. N.; COGGINS C. W. JR.; RADLICK P. C. 《Journal of experimental botany》1965,16(3):511-517
Evidence for the non-indolic nature of the new citrus auxinis presented on the basis of fluorometric properties, thin-layerchromatography, Ehrlich's colour reaction, paper electrophoresis,and the infra-red spectra determinations. Citrus auxin had alower Rf in TLC than IAA, did not give the typical indole reactionwith Ehrlich's reagent, and behaved differently in electrophoresis.The infra-red spectra also provided preliminary informationconcerning chemical structure. The hypothesis that indolic compoundsconstitute the only natural auxins in higher plants should berevised in view of this evidence that a non-indole auxin existsin higher plants. 相似文献
6.
The Avian Shoulder: An Experimental Approach 总被引:2,自引:0,他引:2
GOSLOW G. E. JR.; DIAL K. P.; JENKINS F. A. JR. 《Integrative and comparative biology》1989,29(1):287-301
SYNOPSIS. This essay is in two parts. The first describes functionalstudies of the shoulder in modern vertebrates that led to theformulation of the hypotheses that motor patterns of homologousmuscles have been maintained during the evolution of the tetrapodshoulder, and that a primitive organization of the neural controlcomponents has persisted in derived groups. The second part of this essay focuses upon a longstanding questionin vertebrate evolution: what neuromuscular and musculoskeletalchanges in the tetrapod shoulder accompanied the evolution offlight in birds? The lack of empirical data on shoulder functionin extant birds limited our insight into this question, andprompted our initiation of experimental studies. Preliminarykinematics of the furcula and humerus of European starlings(Sturnus vulgaris) flying in a wind tunnel, as revealed by highspeed cineradiography, are presented. The two halves of thefurcula, which contact the coracoids dorsally, are bent laterallyduring downstroke and medially during upstroke by as much as60% of the intrafurcular resting distance. High speed film andelectromyographic studies of freeflying pigeons (Columba livia)reveal that the supracoracoideus muscle is strongly activatedduring wing elevation and, as predicted from studies of Varanusand Didelphis, an additional activation burst occurs at mid-downstrokein 48% of the recordings. 相似文献
7.
SYNOPSIS. Quantitative analysis of low-level (mostly subspecific)geographic differentiation of aposematic color patterns in threegroups of forest butterflies (Heliconiini, Ithomiinae, and Troidini),in 1,550 half-degree quadrants throughout the Neotropics, indicatesfour principal species-endemicity regions, 44 principal subspecies-endemicitycenters, and 300 quadrants of subspecies hybridization. Theendemism centers are different in outline but nearly identicalin position for each of the three ecologically different groups.Basic data on present and past physical environments (climate,soils, geomorphology, and vegetation structures), when integratedinto maps, show 1) regions of favorable ecological conditionsfor forest organisms in the present, 2) regions of environmentalconformities and rapid transitions in the present, and 3) regionsof high probability for humid forest persistence during themajor landscape changes at the end of the last glacial period,13,00020,000 yr ago. Species diversity (a local phenomenon)is readily related to favorable modern ecological conditionsand environmental microheterogeneity, promoted by unpredictablemild disturbance. Biogeographical patterns of evolution at thelevel of regional subspecies correlate very well with presumedpaleoecological conditions. The biogeographical data also supportan hypothesis of differentiation resulting from areas of rapidenvironmental change; most of the hybridization quadrants fallwithin transition zones. There is lower correlation betweencenters of endemism and zones of en vironmental conformity,but some individual cases of differentiation in the butterfliesmay be adequately explained by modern ecological factors. Speciespresently undergoing differentiation in isolated habitat islands(Amazonian savannas and high-altitude Andean forests and paramos)may offer the best opportunities for observation of evolutionaryprocesses in the present. 相似文献
8.
Summary In ethanol production with immobilized yeast a major problem is the provision of nutrients to these highly concentrated cells. O2 being one of the nutrients of utmost importance to yeast cells, was fed into a column packed with beads with a cell loading of more than 40 g/l. Since addition of large volume of air or O2 to a cylindrical column reactor would aggravate the problems of pressure build up and channelling caused by the evolving CO2 gas, a tapered-column reactor and pulsed flow of oxygen gas was used. The supplement of O2 gas to the tapered column increased the productivity from 21.1 g ethanol x (l gel x h)-1 to 26.7 g x (l gel x h)-1, when the ethanol concentration at the outlet was about 80 g/l. The yield coefficient of ethanol was also increased from 0.41 g ethanol/g glucose to 0.43 after O2 supplement was started. The effects of frequency and duration of O2 supplement were also determined. 相似文献
9.
HEATHER C. TIPTON VICTORIA J. DREITZ PAUL F. DOHERTY JR. 《The Journal of wildlife management》2008,72(4):1001-1006
Abstract: Concern over the decline of grassland birds has spurred efforts to increase understanding of grassland bird-habitat relationships. Previous studies have suggested that black-tailed prairie dogs (Cynomys ludovicianus) provide important habitat for shortgrass prairie avifauna, such as mountain plover (Charadrius montanus) and western burrowing owl (Athene cunicularia hypugaea), although such studies are lacking in Colorado (USA). We used methods to estimate occupancy (ψ) of mountain plover and burrowing owl on prairie dog colonies and other shortgrass prairie habitats in eastern Colorado. Mountain plover occupancy was higher on prairie dog colonies (ψ = 0.50, 95% CI = 0.36–0.64) than on grassland (ψ = 0.07, 95% CI = 0.03–0.15) and dryland agriculture (ψ = 0.13, 95% CI = 0.07–0.23). Burrowing owl occupancy was higher on active prairie dog colonies (ψ = 0.80, 95% CI = 0.66–0.89) compared with inactive colonies (ψ = 0.23, 95% CI = 0.07–0.53), which in turn was much higher than on grassland (ψ = 0.01, 95% CI = 0.00–0.07) and dryland agriculture (ψ = 0.00, 95% CI ψ 0.00–0.00). Mountain plover occupancy also was positively correlated with increasing amounts of prairie dog colony in the landscape. Burrowing owl occupancy was negatively correlated with increasing amounts of prairie dog colony in the surrounding landscape. Our results suggest that actions to conserve mountain plovers and burrowing owls should incorporate land management to benefit prairie dogs. Because managing for specific colony attributes is difficult, alternative management that promotes heterogeneity may ensure that suitable habitat is available for the guild of grassland inhabitants. 相似文献