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One hundred and ninety four Aeromonas isolates (99 from food and 95 from clinical sources) were analyzed as to the species involved and the toxins produced. Of the clinical isolates of Aeromonas, 29.4% were enterotoxigenic, 43.1% were hemolytic and 89% were cytotoxigenic. Among the food isolates, 18.2% were enterotoxigenic, 17.1% were hemolytic and 72.7% were cytotoxigenic. Aeromonas sobria and Aeromonas veronii produced more enterotoxin and cytotoxin than the other isolates, whereas A. veronii and Aeromonas salmonicida produced cell-free hemolysin. Most of the isolates produced cytotoxins (81%) active on Vero (green monkey kidney) and Chinese hamster ovary cells, but only the culture supernatant of A. sobria produced vacuolation in these cell lines. 相似文献
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Stefania Carbone Rosabel Lobo Alvarez Annalisa Lamacchia Asuncion Almenar Gil Raquel Martin Hernandez Jose Luis Lopez Guerra Hugo Marsiglia 《Reports of Practical Oncology and Radiotherapy》2012,17(6):363-366
BackgroundSquamous cells are normally not found inside the breast. Therefore, a primary squamous cell carcinoma of the breast is an exceptional phenomenon and the management of this type of disease is still debated.AimClinical outcome assessment of a patient with squamous cell carcinoma of the breast.Materials and methodsWe report a case of primary squamous cell carcinoma of the breast (T1cN0M0) in a 51-years-old woman who underwent breast conserving surgery plus adjuvant chemotherapy and radiation therapy (RT).ResultsWith a follow up of 43 months, the patient is alive with no evidence of local or distant recurrence. The patient had Grade 2 acute skin toxicity. No late skin or respiratory toxicity was observed.ConclusionsPure primary squamous cell carcinoma of the breast is a rare and aggressive disease, often treatment-refractory. Our case shows that the addition of RT after breast conserving surgery, allows to achieve a high local control without adding severe toxicity. A multidisciplinary approach seems to be the optimal management for early stages in this rare disease. 相似文献
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Summary A laboratory study was made of the losses of nitrogen through ammonia volatilization from four flooded, tropical soils. The
soils used varied considerably in pH, organic matter content, and cation exchange capacity. Losses were measured from the
unamended soils, and from ammonium sulphate and urea-treated soils. Two rates of nitrogen application (approximately 50 and
200 kg/ha N) and two methods of application (simulated field broadcast and fertilizer incorporation) of the nitrogen were
used.
Losses of ammonia were detected for each of the unamended soils, including an acid sulphate soil of pH 3.6. Increased application
of both ammonium sulphate and urea resulted in increased losses of ammonia through volatilization. Incorporation of the nitrogen
into the mud of the flooded soils significantly decreased losses due to volatilization. It was concluded that the initial
or ‘aerobic’ pH of the soils was the soil characteristic most closely related to the magnitude of losses due to volatilization. 相似文献
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Induction of apoptosis in Vero cells by Aeromonas veronii biovar sobria vacuolating cytotoxic factor
Martins LM Catani CF Falcón RM Carbonell GV Azzoni AA Yano T 《FEMS immunology and medical microbiology》2007,49(2):197-204
Recently, a cytotoxin named vacuolating cytotoxic factor (VCF) in Aeromonas sobria and Aeromonas veronii biovar sobria was described. We have now purified this factor using ion metallic affinity chromatography. The VCF is a nonhemolytic enterotoxin that acts as a serine protease. The toxin can be partially neutralized by serum antiaerolysin and it induced not only cytoplasmatic vacuole formation, but also mitochondrial disorders that must be signaling the apoptotic pathways, leading to Vero (African green monkey kidney) cell death. These results suggest that the VCF is a virulence factor of these bacteria, participating in the processes of human disease provoked by preformed toxins in food and infection. 相似文献
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Castellanos L de Correa RS Martínez E Calderon JS 《Zeitschrift für Naturforschung. C, Journal of biosciences》2002,57(7-8):575-578
Two new oleanane-type triterpenes, characterized as 3-oxo-11alpha,12alpha-epoxy-oleanan-28,13beta-olide and 3-oxo-olean-11-en-28,13beta-olide , were isolated from the fruits and seeds of Cedrela montana (Meliaceae). In addition, the known compounds oleanonic acid, a mixture of beta-sitosterol and stigmasterol, and the limonoid photogedunin were also isolated. The structures of the new compounds were established by spectroscopic methods, including 2D NMR. 相似文献
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