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1.
In this paper the recent population changes of the Wild Boar in different European countries is analysed through the study of hunting statistics. A simultaneous increase in numbers is observed throughout the whole area during the period 1965–1975. From 1975 onwards the population stabilizes itself apart from in peripheral areas like Finland. Potentially favourable factors which play a part in this process are discussed and certain reproductive and dispersive characteristics which favour its invasive behaviour are discussed. 相似文献
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The 230-kDa gamete surface protein of Plasmodium falciparum is also a target for transmission-blocking antibodies 总被引:14,自引:0,他引:14
I A Quakyi R Carter J Rener N Kumar M F Good L H Miller 《Journal of immunology (Baltimore, Md. : 1950)》1987,139(12):4213-4217
Immunization with extracellular sexual stages of the malaria parasites can induce the production of antibodies which block the development of the parasites in the midgut of a mosquito after a blood meal. We have generated a number of monoclonal antibodies against gametes and zygotes of the human malaria Plasmodium falciparum. Two monoclonal antibodies (mAb) reacting with a 230-kDa gamete surface protein (mAb 1B3 and 2B4 both isotype IgG2a) were found to block transmission of P. falciparum to mosquitoes. Blocking was complement dependent and this was verified in vitro by the rapid lysis of newly formed gametes and zygotes in the presence of the mAb and active complement. Both mAb reacted by immunofluorescence with the surface of gametes and zygotes from isolates of P. falciparum from various geographical areas. Each mAb immunoprecipitated a 230-kDa protein from 125I-labeled surface proteins of newly formed gametes and zygotes and immunoblotted a protein doublet of about molecular mass 260 and 230 kDa from gametocytes and gametes of P. falciparum. Only the 230-kDa protein is expressed on the surface of newly formed macrogametes and zygotes. The 230-kDa gamete surface protein forms a molecular complex with two proteins of 48 and 45 kDa. The 48- and 45-kDa gamete surface proteins have previously been shown to be targets of mAb which block infectivity of P. falciparum to mosquitoes. The present study now demonstrates that antibodies against the 230-kDa gamete surface protein block transmission of P. falciparum to mosquitoes. The 230-kDa gamete protein is thus a potential candidate for a gamete vaccine. 相似文献
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Extensive multiple test centre evaluation of the VecTest malaria antigen panel assay 总被引:3,自引:0,他引:3
Ryan JR Davé K Collins KM Hochberg L Sattabongkot J Coleman RE Dunton RF Bangs MJ Mbogo CM Cooper RD Schoeler GB Rubio-Palis Y Magris M Romer LI Padilla N Quakyi IA Bigoga J Leke RG Akinpelu O Evans B Walsey M Patterson P Wirtz RA Chan AS 《Medical and veterinary entomology》2002,16(3):321-327
To determine which species and populations of Anopheles transmit malaria in any given situation, immunological assays for malaria sporozoite antigen can replace traditional microscopical examination of freshly dissected Anopheles. We developed a wicking assay for use with mosquitoes that identifies the presence or absence of specific peptide epitopes of circumsporozoite (CS) protein of Plasmodium falciparum and two strains of Plasmodium vivax (variants 210 and 247). The resulting assay (VecTest Malaria) is a rapid, one-step procedure using a 'dipstick' test strip capable of detecting and distinguishing between P. falciparum and P. vivax infections in mosquitoes. The objective of the present study was to test the efficacy, sensitivity, stability and field-user acceptability of this wicking dipstick assay. In collaboration with 16 test centres world-wide, we evaluated more than 40 000 units of this assay, comparing it to the standard CS ELISA. The 'VecTest Malaria' was found to show 92% sensitivity and 98.1% specificity, with 97.8% accuracy overall. In accelerated storage tests, the dipsticks remained stable for > 15 weeks in dry conditions up to 45 degrees C and in humid conditions up to 37 degrees C. Evidently, this quick and easy dipstick test performs at an acceptable level of reliability and offers practical advantages for field workers needing to make rapid surveys of malaria vectors. 相似文献
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TANATIN BI 《Mikrobiologiia》1951,20(6):506-511
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ANDRÉIA SILVA FLORES RÉA M. CORRÊA ELIANA R. FORNI-MARTINS ANA M. G. AZEVEDO TOZZI 《Botanical journal of the Linnean Society. Linnean Society of London》2006,151(2):271-277
Chromosome numbers were counted for 23 species of Crotalaria native to Brazil. Among these data there were new counts for 15 taxa, and some confirmed previous reports or represented numbers that were different from those cited previously. The chromosome numbers most frequently found were 2 n = 16 and 2 n = 32. Only C. incana L. had 2 n = 14 and C. tweediana Benth. had 2 n = 54. The counts 2 n = 32 and 54 were found in species of section Calycinae and 2 n = 16 and 14 in species of section Chrysocalycinae . The data revealed the importance of chromosomal parameters in the characterization of sections Calycinae and Chrysocalycinae in Brazil. We discuss the systematic significance and evolutionary aspects for the genus, comparing the results with the two sections that are native in Brazil. © 2006 The Linnean Society of London, Botanical Journal of the Linnean Society , 2006, 151 , 271–277. 相似文献
8.
Ricci GC De Souza-Kaneshima AM Felismino MF Mendes-Bonato AB Pagliarini MS Do Valle CB 《Journal of genetics》2011,90(2):289-294
A total of 44 accessions of Brachiaria decumbens were analysed for chromosome count and meiotic behaviour in order to identify potential progenitors for crosses. Among them,
15 accessions presented 2n = 18; 27 accessions, 2n = 36; and 2 accessions, 2n = 45 chromosomes. Among the diploid accessions, the rate of meiotic abnormalities was low, ranging from 0.82% to 7.93%. In
the 27 tetraploid accessions, the rate of meiotic abnormalities ranged from 18.41% to 65.83%. The most common meiotic abnormalities
were related to irregular chromosome segregation, but chromosome stickiness and abnormal cytokinesis were observed in low
frequency. All abnormalities can compromise pollen viability by generating unbalanced gametes. Based on the chromosome number
and meiotic stability, the present study indicates the apomictic tetraploid accessions that can act as male genitor to produce
interspecific hybrids with B. ruziziensis or intraspecific hybrids with recently artificially tetraploidized accessions. 相似文献
9.
The genetic variability at six cloned minisatellite loci was analyzed in
minke whale populations from the North Atlantic, North Pacific, and
Antarctic Oceans. Three loci displayed only a few different alleles in each
of the three populations, with heterozygosity ranging from 0.00 to 0.47,
and three loci revealed many different alleles in at least two fo the three
populations, with heterozygosity ranging up to 0.98. Using small sample
sizes, samples from two adjacent Antarctic Management Areas were not found
to differ significantly in allele frequencies at any of the six loci. The
use of principal coordinate analysis to detect multilocus disequilibria was
explored. No significant evidence was found of intrapopulation
heterogeneity within the pooled Antarctic sample. Pronounced interoceanic
differences were observed at every locus, confirming the existence of
genetic isolation found earlier using more conventional marker systems. The
populations from the three oceans appear to have diverged to such a degree
that the hypervariable loci have had time to evolve independently and
arrive at different evolutionary stages in different populations. The
frequency of undetected "null" alleles is remarkably high in minke whale
populations compared to human populations and is probably a result of the
cloning protocol used. Minisatellite loci are shown to provide a powerful
population genetic tool, supplying levels of resolution appropriate to
different degrees of evolutionary divergence.
相似文献
10.
Human T cell recognition of polymorphic epitopes from malaria circumsporozoite protein 总被引:5,自引:0,他引:5
A S de Groot A H Johnson W L Maloy I A Quakyi E M Riley A Menon S M Banks J A Berzofsky M F Good 《Journal of immunology (Baltimore, Md. : 1950)》1989,142(11):4000-4005
Lymphocytes obtained from forty individuals living in a malaria endemic area of West Africa were tested for in vitro proliferative responses to peptides representing variant regions of the immunodominant T cell domain of the circumsporozoite protein (amino acids 326 to 345, referred to as Th2R, and 361 to 380, referred to as Th3R) from three distinct strains of Plasmodium falciparum. A total of 83% of the individuals responded to at least one of the six peptides tested, confirming that these epitopes are immunodominant. A much greater number of individuals than expected by chance (32% of the responders to Th2R and 27% of the responders to Th3R) reacted to all three of the variant peptides for that epitope, indicating interdependency of the T cell responses, suggestive of cross-reactivity. Nevertheless, some subjects' T cells were clearly able to distinguish each variant peptide from the others. Using EBV transformed B cells, lymphocytes from 10 of the individuals were HLA typed. In this small group, HLA DRw13 was associated with a positive response to any of the peptides, whereas there was a negative association between DQw3 and response to any of the peptides. These results, although limited by the small sample size, suggest that recognition of T epitopes may be Ir gene linked. Our findings suggest that it may be possible to broaden the immunogenicity of an anti-sporozoite malaria vaccine. 相似文献