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Major ecological problems of our polluted troposphere includeairborne toxic chemicals, acid rain and photochemical smog,all three of which are now recognized as being closely relatedchemical phenomena. We also recognize that inorder to developcost-effective strategies for their control, which protect publichealth and the environment, there must be close scientific interactionsbetween chemists and biological scientists. For example, ofrapidly emerging importance is the development of risk assessmentevaluations for specific aspects of each of these problem areas.In preparing such assessments, chemists must define the "exposure,"and biological scientists the "effects." In this paper, I discuss an example of how such close interactionsproved indispensible in our search for atmospheric mutagensand carcinogens. Thus, an integrated chemical/ microbiologicalprocedure for the isolation and identificationof particulatechemical mutagens in respirable diesel soot and ambient particlesis described. Emphasis is placed on our use of the short-term,Ames Salmonella typhimurium bacterial mutagenicity test as arapid, and relatively inexpensive, means of following the biologicalactivities of these environmental mutagens through the chemicalsteps of their separation, isolation and identification fromhighly complex environmental samples. Possible mechanisms offormation of these particulate mutagens are discussed. Theyinclude the reactions of polycyclic aromatic hydrocarbons presenton the surfaces of combustion-generated particles with gaseousco-pollutants such as nitrogen dioxide plus nitric acid, andozone. In discussing this research on a societally "relevant" problem,we illustrate the importance of "Science as a Way of Knowing."We further suggest that this integrated approach to scientificproblem solving by chemical and biological scientists mightserve as an example of a discussion topic on human ecology forundergraduate courses in the natural sciences. 相似文献
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ZACHARY W. FRATTO VALERIE A. BARKO PHILLIP R. PITTS STEVEN L. SHERIFF JEFFREY T. BRIGGLER KEVIN P. SULLIVAN BRIAN L. MCKEAGE TOM R. JOHNSON 《The Journal of wildlife management》2008,72(7):1628-1633
Abstract Baited and unbaited hoop-nets commonly are used to capture catfish in lotic and lentic systems. Turtle bycatch and post-capture mortality has been problematic during catfish surveys in Missouri, USA, most recently in the Gasconade River, Gasconade and Osage counties. We evaluated 3 modified hoop-net designs that would reduce turtle bycatch without reducing catfish capture in the Gasconade River during 15 May-15 July 2006 after pilot study evaluation of 5 hoop-net designs in April 2006. We deployed modified and control-nets in blocks for 48 hours to evaluate differences in turtle and catfish catch rate, as well as abundance, size, and mortality rate of turtle bycatch. The chimney design reduced turtle bycatch by 84% when compared to the control, without decreasing the number or average size of captured flathead catfish (Pylodictis olivaris). Environmental conditions that affected turtle mortality included Secchi disc transparency, temperature, dissolved oxygen, and stream river depth. This is the first known attempt to create turtle exclusion or escapement devices for hoop-nets deployed in freshwater systems. Biologists using hoop-nets to sample aquatic vertebrates in moderate to large river systems will benefit from our study. The application of this methodology will reduce turtle bycatch mortality, especially when sampling is conducted in high water temperatures. 相似文献
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Pre‐wintering conditions and post‐winter performance in a solitary bee: does diapause impose an energetic cost on reproductive success? 下载免费PDF全文
FABIO SGOLASTRA XAVIER ARNAN THERESA L. PITTS‐SINGER STEFANO MAINI WILLIAM P. KEMP JORDI BOSCH 《Ecological Entomology》2016,41(2):201-210
1. Diapause is a dynamic process of low metabolic activity that allows insects to survive periods of harsh conditions. Notwithstanding the lowered metabolism, and because diapausing insects have no access to food, diapause has an energetic cost that may affect post‐diapause performance. 2. Previous studies on the solitary bee Osmia lignaria have shown that prolonged pre‐wintering periods (the time during which individuals already in diapause remain at warm temperatures) are associated with elevated lipid consumption, fat body depletion, and body weight loss. The present study investigated whether prolonged pre‐wintering also affects reproduction, i.e. whether the costs associated with diapause could have an effect on post‐diapause performance in this species. 3. Females were exposed to a range of pre‐wintering conditions, and ovary development and individual post‐wintering performance were monitored throughout their adult life span. 4. No evidence of an effect of pre‐wintering duration on post‐diapause reproductive success was found. Expected differences in the timing of establishment were not observed because ovary maturation was, surprisingly, not arrested during pre‐wintering. Prolonged pre‐wintering duration did not result in decreased life span, probably because emerging females could rapidly replenish their metabolic reserves through feeding. However, there was a very strong effect of the duration of the pre‐emergence period on the likelihood of nest establishment. 5. Longevity, the main factor determining fecundity in Osmia, is subjected to high levels of intrinsic variability, even among females of similar size exposed to identical conditions during development and nesting. 相似文献
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NATHAN R. FRANSSEN KEITH B. GIDO CHRISTOPHER S. GUY JEFF A. TRIPE SALLY J. SHRANK TIMOTHY R. STRAKOSH KATIE N. BERTRAND COURTNEY M. FRANSSEN KRISTEN L. PITTS CRAIG P. PAUKERT 《Freshwater Biology》2006,51(11):2072-2086
1. Floods are major disturbances to stream ecosystems that can kill or displace organisms and modify habitats. Many studies have reported changes in fish assemblages after a single flood, but few studies have evaluated the importance of timing and intensity of floods on long‐term fish assemblage dynamics. 2. We used a 10‐year dataset to evaluate the effects of floods on fishes in Kings Creek, an intermittent prairie stream in north‐eastern, Kansas, U.S.A. Samples were collected seasonally at two perennial headwater sites (1995–2005) and one perennial downstream flowing site (1997–2005) allowing us to evaluate the effects of floods at different locations within a watershed. In addition, four surveys during 2003 and 2004 sampled 3–5 km of stream between the long‐term study sites to evaluate the use of intermittent reaches of this stream. 3. Because of higher discharge and bed scouring at the downstream site, we predicted that the fish assemblage would have lowered species richness and abundance following floods. In contrast, we expected increased species richness and abundance at headwater sites because floods increase stream connectivity and create the potential for colonisation from downstream reaches. 4. Akaike Information Criteria (AIC) was used to select among candidate regression models that predicted species richness and abundance based on Julian date, time since floods, season and physical habitat at each site. At the downstream site, AIC weightings suggested Julian date was the best predictor of fish assemblage structure, but no model explained >16% of the variation in species richness or community structure. Variation explained by Julian date was primarily attributed to a long‐term pattern of declining abundance of common species. At the headwater sites, there was not a single candidate model selected to predict total species abundance and assemblage structure. AIC weightings suggested variation in assemblage structure was associated with either Julian date or local habitat characteristics. 5. Fishes rapidly colonised isolated or dry habitats following floods. This was evidenced by the occurrence of fishes in intermittent reaches and the positive association between maximum daily discharge and colonisation events at both headwater sites. 6. Our study suggests floods allow dispersal into intermittent habitats with little or no downstream displacement of fishes. Movement of fishes among habitats during flooding highlights the importance of maintaining connectivity of stream networks of low to medium order prairie streams. 相似文献
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