全文获取类型
收费全文 | 1950篇 |
免费 | 101篇 |
国内免费 | 2篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 17篇 |
2020年 | 11篇 |
2019年 | 20篇 |
2018年 | 23篇 |
2017年 | 20篇 |
2016年 | 52篇 |
2015年 | 56篇 |
2014年 | 94篇 |
2013年 | 147篇 |
2012年 | 122篇 |
2011年 | 159篇 |
2010年 | 88篇 |
2009年 | 69篇 |
2008年 | 108篇 |
2007年 | 90篇 |
2006年 | 124篇 |
2005年 | 132篇 |
2004年 | 122篇 |
2003年 | 125篇 |
2002年 | 118篇 |
2001年 | 17篇 |
2000年 | 19篇 |
1999年 | 16篇 |
1998年 | 28篇 |
1997年 | 27篇 |
1996年 | 17篇 |
1995年 | 21篇 |
1994年 | 25篇 |
1993年 | 7篇 |
1992年 | 27篇 |
1991年 | 10篇 |
1990年 | 13篇 |
1989年 | 13篇 |
1988年 | 13篇 |
1987年 | 11篇 |
1986年 | 17篇 |
1985年 | 6篇 |
1984年 | 7篇 |
1983年 | 11篇 |
1982年 | 14篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1976年 | 4篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
排序方式: 共有2053条查询结果,搜索用时 15 毫秒
1.
2.
3.
Katsutoshi Yoshizato Sakaé Kikuyama Nobuyuki Shioya 《Biochimica et Biophysica Acta (BBA)/General Subjects》1980,627(1):23-29
Human dermal fibroblasts were obtained by harvesting outgrowing cells from the dermal tissue explants and cultured in Dulbecco's modified Eagle medium containing 10% fetal calf serum. After the cells reached confluency, culture was continued in the medium containing calf serum which was deprived of thyroid hormone by the treatment with activated charcoal. These fibroblasts were responsive to exogeneously added thyroid hormone (triiodothyronine) at physiological concentrations, resulting in enhanced utilization of glucose and production of lactate. This stimulation by thyroid hormone was dependent upon the length of exposure to the hormone and its concentration.The hormone did not show any effects on cellular DNA and protein content. The experimental system described above seems to be easy to reconstitute and should be useful for the elucidation of the mechanism of thyroid hormone action. 相似文献
4.
5.
Attempts were made to induce azobenzenearsonate (ABA)-specific helper cell responses in A/J mice. These were measured by an increase in TNP plaque-forming cells following administration of the double hapten conjugate ABA-bovine serum albumin-TNP. Immunization with ABA coupled homologous immunoglobulin or spleen cells produced ABA-specific help only when the same carrier was used to boost. Hapten-specific help was achieved by two injections of ABA-N-acetyltyrosine in complete Freund's adjuvant 5 weeks apart. This help was passively transferable by T cells as shown by its elimination with anti-Thy 1.2 serum and complement treatment. The presence of the major ABA cross-reactive idiotype (CRI) on these T helper cells could be similarly shown by the elimination of help when the cells were treated with rabbit anti-CRI antibody and complement prior to passive transfer. The same treatment did not effect ABA-specific helper activity of CBA/J mice. 相似文献
6.
Masafumi Komiya Shigehiro Asano Nobuyuki Koike Erina Koga Junetsu Igarashi Shogo Nakatani Yoshiaki Isobe 《Bioorganic & medicinal chemistry》2012,20(23):6840-6847
Based on 2-(4-phenoxybenzoyl)-5-hydroxyindole (2), a novel structural class of CaMKII inhibitors were synthesized and further optimized. The strong acidity of the hydroxyl group and the lipophilic group at the 4 and 6-positions were found to be necessary for strong CaMKII inhibition. Compound 25 was identified as a promising compound with 50-fold more potent inhibitory activity for CaMKII than 2. Compound 25 also showed high selectivity for CaMKII over off-target kinases. 相似文献
7.
8.
Fruit trees have a long juvenile phase. For example, the juvenile phase of apple (Malus × domestica) generally lasts for 5–12 years and is a serious constraint for genetic analysis and for creating new apple cultivars through cross‐breeding. If modification of the genes involved in the transition from the juvenile phase to the adult phase can enable apple to complete its life cycle within 1 year, as seen in herbaceous plants, a significant enhancement in apple breeding will be realized. Here, we report a novel technology that simultaneously promotes expression of Arabidopsis FLOWERING LOCUS T gene (AtFT) and silencing of apple TERMINAL FLOWER 1 gene (MdTFL1‐1) using an Apple latent spherical virus (ALSV) vector (ALSV‐AtFT/MdTFL1) to accelerate flowering time and life cycle in apple seedlings. When apple cotyledons were inoculated with ALSV‐AtFT/MdTFL1 immediately after germination, more than 90% of infected seedlings started flowering within 1.5–3 months, and almost all early‐flowering seedlings continuously produced flower buds on the lateral and axillary shoots. Cross‐pollination between early‐flowering apple plants produced fruits with seeds, indicating that ALSV‐AtFT/MdTFL1 inoculation successfully reduced the time required for completion of the apple life cycle to 1 year or less. Apple latent spherical virus was not transmitted via seeds to successive progenies in most cases, and thus, this method will serve as a new breeding technique that does not pass genetic modification to the next generation. 相似文献
9.
10.