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Production of vitamin B12 in an upflow anaerobic filter continuous reactor using Acetobacterium sp. 总被引:2,自引:0,他引:2
Alberto Emilio Bainotti Belén Estebanez Hisashi Nagadomi Naomichi Nishio 《Biotechnology letters》2000,22(6):503-508
The accumulation of biofilm by Acetobacterium sp. during continuous culture in an upflow anaerobic filter (UAF) growing on methanol-formate was the result of space velocity and inlet concentrations of substrate and Co+2. To achieve good development of biofilm, a space velocity of 0.38 h–1, inlet substrate concentrations of 125 mM of both methanol and formate, and Co+2 at 0.16 mM were required. Cell productivities in the effluent of the UAF-reactor were about 6-fold higher than in chemostat cultures (0.20 g l–1 h–1 for UAF and 0.035 g l–1 h–1 for chemostat) (previous studies), and the maximum vitamin B12 specific concentration was 5.1 mg g cell–1. 相似文献
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Nagadomi H. Takahasi T. Sasaki K. Yang H.C. 《World journal of microbiology & biotechnology》2000,16(1):57-62
A denitrifying photosynthetic bacterium (PSB), Rhodobacter sphaeroides S was immobilized on a porous ceramic plate (10×10×0.5cm) using a small amount of agar. In the bioreactor (3.5 l liquid) using this plate, repeated batch treatments of sewage wastewater (acetic acid as a carbon source) were carried out under aerobic conditions (DO 5mg/l) for two weeks. The simultaneous and effective removal of chemical oxygen demand (COD), ammonium, and nitrate was observed. In continuous treatment at a relatively high dilution rate of 0.20 h–1 (retention time, five hours), the same simultaneous treatments were observed for about one month. 相似文献
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Hisashi Nagadomi Tomohiro Kitamura Masanori Watanabe Ken Sasaki 《Biotechnology letters》2000,22(17):1369-1374
Simultaneous aerobic treatment of COD, phosphate, nitrate and H2S in a synthetic sewage wastewater was carried out using porous ceramic immobilized photosynthetic bacteria, Rhodobacter sphaeroidesS, Rb. sphaeroidesNR-3 and Rhodopseudomonas palustris. In the batch treatment, effective simultaneous removal of COD (89%), phosphate (77%), nitrate (99%) and H2S (99.8%) was observed after 48 h. In semi-continuous treatments with dilution rates of 0.17 to 0.75 day–1under aerobic conditions, simultaneous removal of these four components was also observed after about one month. 相似文献
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