Properties of monoclonal antibodies specific for determinants of a protein antigen, myoglobin

Monoclonal hybridoma antibodies specific for the protein antigen sperm whale myoglobin were produced using hyperimmune spleen cells from mice with the genetic trait of high responsiveness to myoglobin. Antibodies from the several clones tested were found to produce linear Scatchard plots, as predicted for homogeneous antibodies, and to possess high affinities for the immunogen (KA congruent to 10(9) M-1). None of the monoclonal antibodies tested reacted with either fragment (1-55) or fragment (132-153) of sperm whale myoglobin. Competitive binding assays using human and horse myoglobins suggested that several of these monoclonal antibodies, which can readily distinguish these myoglobins, recognize different antigenic determinants on the myoglobin molecule. Studies using additional myoglobin sequence variants as competitors should be able to more closely define these antigenic determinants.     

Entangled in the imagination: New middle-class apprehensions in an Indian theme park

By analysing a visit to Ramoji Film City, a theme park in Hyderabad, by a group of youths, this essay examines how transterritorial flows of imagery are socially contextualized by India's new middle classes. A theme park is a spatial arrangement thatincorporatesimaginary worlds and makes them available to experiencein a contextless ‘here-and-now’. As such, the theme parkis a stage where the fragileidentity of India's new middle classes is put on trial. Despite the appeal of the theme park for potential middle-class visitors, the visitors I followed appeared largely uninspired by the imagery of the far away and past in Film City, and they found it difficult to incorporate their visit to the theme park as a meaningful social practice. The case of Ramoji Film City shows how the social situatedness of the subject determines the significance of the imagination in the transnational world.     

Early growth delay in diabetic pregnancy: relation to psychomotor development at age 4

Ninety nine consecutive insulin dependent and 101 non-diabetic pregnant women were examined by ultrasonograph to assess early fetal growth. In 42 of the diabetic mothers and three of the non-diabetic mothers the scan showed early intrauterine growth delay. At 4-5 years of age all children available for study were evaluated by the Denver developmental screening test. Only 23 of the 34 children of diabetic mothers with early intrauterine growth delay had normal test scores compared with 46 of the 50 children of diabetic mothers with normal intrauterine growth. The children failed in personal-social development, gross motor development, and particularly in language and speech development. Children of diabetic mothers with normal early fetal growth had scores very similar to those of the children of non-diabetic mothers, of whom 76 of the 86 tested had normal scores.This study suggests that children with a history of growth delay in early diabetic pregnancy should be screened for possible developmental impairment.     

Use of aminopterin in selecting electrically active neuroblastoma cells

A marked increase in electrical excitability and process formation occurs in the N-18 clone of mouse neuroblastoma as these cells go from the logarithmic phase of growth to the stationary state in confluent cultures. Even more excitable cells can be selected by growth in culture medium containing 10⁻⁵ M aminopterin which kills about 90% of the cells. Clone 1A-103 does not develop significant processes or exhibit marked electrical excitability under any of the culture conditions studied. Thus, our results show that one or more of the steps required for generation of the action potential is sensitive to regulation in cultured cells. Methods are presented for obtaining populations of either electrically passive cells or electrically excitable cells which can easily be maintained for several weeks. Clones differ markedly in their capacity to extend processes and their ability to generate action potentials.