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排序方式: 共有1117条查询结果,搜索用时 190 毫秒
1.
Changes in Targeting Efficiencies of Proteins to Plant Microbodies Caused by Amino Acid Substitutions in the Carboxy-terminal Tripeptide 总被引:7,自引:0,他引:7
Hayashi Makoto; Aoki Masahiro; Kondo Maki; Nishimura Mikio 《Plant & cell physiology》1997,38(6):759-768
It has been demonstrated that the carboxyl terminus of microbodyenzymes functions as a targeting signal to microbodies in higherplants. We have examined an ability of 24 carboxy-terminal aminoacid sequences to facilitate the transport of a cytosolic passengerprotein, ß-glucuroni-dase, into microbodies in greencotyledonary cells of trans-genic Arabidopsis. Immunoelectronmicroscopic analysis revealed that carboxy-terminal tripeptidesequences of the form [C/A/S/P]-[K/R]-[I/L/M] function as amicrobody-targeting signal, although tripeptides with prolineat the first amino acid position and isoleucine at the carboxylterminus show weak targeting efficiencies. All known micro-bodyenzymes that are synthesized in a form similar in size to themature molecule, except catalase, contain one of these tripeptidesequences at their carboxyl terminus. (Received April 14, 1997; Accepted April 8, 1997) 相似文献
2.
Yasuko Koumoto Ryuji Tsugeki Tomoo Shimada Hitoshi Mori Maki Kondo Ikuko Hara-Nishimura Mikio Nishimura 《The Plant journal : for cell and molecular biology》1996,10(6):1119-1125
Chaperonin (Cpn) is one of the molecular chaperones. Cpn10 is a co-factor of Cpn60, which regulates Cpn60-mediated protein folding. It is known that Cpn10 is located in mitochondria and chloroplasts in plant cells. The Escherichia coli homologue of Cpn10 is called GroES. A cDNA for the Cpn10 homologue was isolated from Arabidopsis thaliana by functional complementation of the E. coli groES mutant. The cDNA was 647 bp long and encoded a polypeptide of 98 amino acids. The deduced amino acid sequence showed approximately 50% identity to mammalian mitochondrial Cpn10s and 30% identity to GroES. A Northern blot analysis revealed that the mRNA for the Cpn10 homologue was expressed uniformly in various organs and was markedly induced by heat-shock treatment. The Cpn10 homologue was constitutively expressed in transgenic tobaccos. Immunogold and immunoblot analyses following the subcellular fractionation of leaves from transgenic tobaccos revealed that the Cpn10 homologue was localized in mitochondria and accumulated at a high level in transgenic tobaccos. 相似文献
3.
Mikio Sato Tadaatsu Nakahara Koichi Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(11):1969-1974
An investigation of succinic acid production from n-paraffin under various culture conditions was carried out with Candida brumptii IFO 0731. Ammonium nitrogen was required for both cell growth and succinic acid production. Favorable culture conditions for succinic acid production were ascertained. The productivity was markedly increased by the additions of CaCO3 and organic nutrients. Under the best condition, the largest quantity of succinic acid production, 23.6 mg/ml, was obtained in a 67% yield from super heavy n-paraffin after 8 days cultivation. 相似文献
4.
The densities of two benthic fishes, the Siberian stone loach (Noemacheilus barbatulus) and the wrinklehead sculpin (Cottus nozawae), and the biomass of their food resources (i.e., periphyton and benthic invertebrates) were compared between forest and grassland streams in northern Hokkaido, Japan, to examine whether riparian deforestation had positive effects on the benthic fishes via enhancement of food availability. The comparisons indicated that riparian vegetation had little influence on periphyton, invertebrates, or fishes. Regression analysis indicated that spatial variations in loach and sculpin densities were explained more by substrate heterogeneity, competitor abundance, or both, rather than by food abundance. However, when the two species were combined as benthic insectivores, a strong correlation was found between total benthic fish density and invertebrate biomass. Our results suggest that, although total benthic fish abundance was food limited, riparian vegetation had no positive effects via food availability on the benthic fishes in our streams. 相似文献
5.
Shinsaku Tokuda Young Hak Kim Hisako Matsumoto Shigeo Muro Toyohiro Hirai Michiaki Mishima Mikio Furuse 《PloS one》2015,10(12)
The relationship between chronic inflammation and cancer is well known. The inflammation increases the permeability of blood vessels and consequently elevates pressure in the interstitial tissues. However, there have been only a few reports on the effects of hydrostatic pressure on cultured cells, and the relationship between elevated hydrostatic pressure and cell properties related to malignant tumors is less well understood. Therefore, we investigated the effects of hydrostatic pressure on the cultured epithelial cells seeded on permeable filters. Surprisingly, hydrostatic pressure from basal to apical side induced epithelial stratification in Madin-Darby canine kidney (MDCK) I and Caco-2 cells, and cavities with microvilli and tight junctions around their surfaces were formed within the multi-layered epithelia. The hydrostatic pressure gradient also promoted cell proliferation, suppressed cell apoptosis, and increased transepithelial ion permeability. The inhibition of protein kinase A (PKA) promoted epithelial stratification by the hydrostatic pressure whereas the activation of PKA led to suppressed epithelial stratification. These results indicate the role of the hydrostatic pressure gradient in the regulation of various epithelial cell functions. The findings in this study may provide clues for the development of a novel strategy for the treatment of the carcinoma. 相似文献
6.
Shin-ya Miyagishima Ryuuichi Itoh Kyoko Toda Haruko Kuroiwa Mikio Nishimura Tsuneyoshi Kuroiwa 《Planta》1999,208(3):326-336
The proliferation cycle of the microbody was studied in the primitive red alga Cyanidioschyzon merolae, which contains one microbody per cell. Cells were synchronized with a dark/light cycle, and the morphology of the microbody
and its interaction with other organelles were observed three-dimensionally by fluorescence microscopy, transmission electron
microscopy, and computer-assisted three-dimensional reconstruction of serial thin sections. The microbody in interphase cells
is a sphere of 0.3 μm in diameter without a core. In M-phase, the microbody passes through a series of irregular shapes, in
the order rod, worm, branched, H-shaped and dumbbell, and symmetric fission occurs just before cytokinesis. The microbody
duplicates its volume in M-phase and three-dimensional quantitative analysis revealed that its surface area increases before
its volume does. The microbody touches the mitochondrion and the chloroplast throughout its proliferation cycle, except briefly
in interphase cells, winding around the divisional plane of the mitochondrion at one phase. Immunocytochemical labeling of
catalase as a marker of matrix proteins of the microbody revealed that the duplication of catalase occurs in tandem with the
volume increase. While no specific apparatus was identified in the microbody divisional areas, we identified an electron-dense
apparatus about 30–50 nm in diameter between the microbody and the mitochondrion that may play a role in segregating the daughter
microbodies. These results are the first characterization to show the morphological changes of one microbody in a one-microbody
alga without proliferation-inducing substrates, which have been used in many studies, and clearly show that two daughter microbodies
arise by binary fission of the pre-existing microbody.
Received: 11 November 1998 / Accepted: 22 December 1998 相似文献
7.
Takahiko Kojima Hironori Kitaguchi Mikio Yasutake Yoshihisa Matsuda 《Inorganica chimica acta》2005,358(13):3592-3600
Two kinds of Cu(II) complexes having 2,2′-bipyridine derivatives with two 1-naphthoylamide groups or two ethyl dimethylmalonylamide moieties at 6 and 6′ positions as ligands were prepared and characterized by X-ray crystallography and spectroscopic methods. Those ligands bound to the Cu(II) centers in a tetradentate fashion including two amide oxygen atoms in the equatorial planes. Those complexes were found to recognize carboxylic acids as guest molecules by coordination and additional non-covalent interactions, including intramolecular π-π interactions or hydrogen bonding. 相似文献
8.
9.
Mikio Shimizu Ryo Okachi Kazuo Kimura Takashi Nara 《Bioscience, biotechnology, and biochemistry》2013,77(8):1655-1661
Penicillin acylase (EC 3.5.1.11) of Kluyvera citrophila KY7844 was purified approximately 120-fold by DEAE-cellulose chromatography, hydroxyapatite chromatography and isoelectro-focusing fractionation. The purified enzyme, with an approximate molecular weight of 63,000, appeared to be homogeneous in disc electrophoretic analysis, and showed isoelectric point (Ip) 8.12 and 13.0 units/mg of specific activity for cephalexin hydrolysis. The Michaelis constant (Km) for cephalexin and for 7-[1-(1H)-tetrazolylacetamido]-desacetoxycephalosporanic acid ((1H) T-7ADCA) was 1.4 mM and 3.6 mM, respectively. This enzyme was capable of producing (1H) T-7ADCA in 80% yield from 1-(1H)-tetrazolylacetate methylester and 7-aminodesacetoxycephalosporanic acid. 相似文献
10.
Izumi Yajima Mikio Nakamura Hidemasa Sakakibara Junichi Ide Tetsuya Yanai Kazuo Hayashi 《Bioscience, biotechnology, and biochemistry》2013,77(8):1755-1760
The aqueous extract of dried bonito (Katsuobushi) was distilled under reduced pressure. The resulting distillate with diethyl ether and the extract was separated into acidic, phenolic, basic and neutral fractions. The neutral fraction was further fractionated into ten sub-fractions by silica gel column chromatography. All these sub-fractions were analyzed by gas chromatography and gas chromatography-mass spectrometry.One hundred and sixty-five compounds were identified and 12 compounds were tentatively identified from the neutral fraction. Among them, 111 compounds were newly identified as flavor components of Katsuobushi. 相似文献