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B J McLeod B R Brinklow J D Curlewis A S Loudon 《Journal of reproduction and fertility》1991,91(1):229-238
Père David's deer hinds were treated with GnRH, administered as intermittent i.v. injections (2.0 micrograms/injection at 2-h intervals) for 4 days, or as a continuous s.c. infusion (1.0 micrograms/h) for 14 days. These treatments were given early (February-March) and late (May-June) in the period of seasonal anoestrus. The administration of repeated injections of GnRH increased mean LH concentrations from pretreatment values of 0.54 +/- 0.09 to 2.10 +/- 0.25 ng/ml over the first 8 h of treatment in early anoestrus, and from 0.62 +/- 0.11 to 2.73 +/- 0.49 ng/ml in late anoestrus. The mean amplitude of GnRH-induced LH episodes was greater (P less than 0.01) in late (4.03 +/- 0.28 ng/ml) than in early (3.12 +/- 0.26 ng/ml) anoestrus, but within each replicate (early or late anoestrus), neither mean LH episode amplitude nor mean plasma LH concentrations differed significantly between the four periods of intensive blood sampling. On the basis of their progesterone profiles, 6/12 hinds had ovulated in response to treatment with injections of GnRH (1/6 in early anoestrus and 5/6 in late anoestrus), and oestrus and a preovulatory LH surge were recorded in all of these animals. Oestrus and a preovulatory LH surge were also recorded in one other animal treated in early anoestrus in which progesterone concentrations remained low. The mean times of onset of oestrus (91.0 +/- 1.00 and 62.4 +/- 0.98 h) and of the preovulatory LH surge (85.8 +/- 3.76 and 59.4 +/- 0.25 h) both occurred significantly earlier (P less than 0.001) in animals treated in late anoestrus. Continuous infusion of GnRH to seasonally anoestrous hinds resulted in an increase in mean plasma LH concentrations, but this response did not differ significantly between early (2.15 +/- 0.28 ng/ml) and late (2.48 +/- 0.26 ng/ml) anoestrus. Ovulation, based on progesterone profiles, occurred in 2/7 hinds in early anoestrus and in 4/6 hinds in late anoestrus. Oestrus was detected in all except one of these hinds. The mean time of onset of oestrus occurred earlier in animals treated in late anoestrus (66.2 +/- 0.32 h and 46.7 +/- 0.67 h, P less than 0.01). The administration of GnRH, given either intermittently or continuously, will induce ovulation in a proportion of seasonally anoestrous Père David's deer. However, more animals ovulate in response to this treatment in late than in early anoestrus (75% compared with 23%). 相似文献
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A study was made of the accuracy of predicting dry matter digestibility (DMD) in vivo of 32 legume samples and 50 grass samples from the dry matter disappearance (DMS) of the samples in pepsin (48 h) followed by Onozuka cellulase (48 h). The effects of grinder screen size, incubation temperature and sample size on the accuracy of predicting dry matter digestibility were examined.The DMD in vivo of legumes and grasses was significantly correlated with DMS at 39° C (r = 0.91 and 0.94) and increasing the incubation temperature to 50° C had no effect on the correlations, although DMS was greater. There was no significant difference between the regression equations for grasses and legumes. The regression equation for the combined 82 samples had an RSD of ± 3.0 digestibility units. The corresponding RSD for regressions based on fermentation with rumen fluid in vitro was ± 2.6 digestibility units. Grinding the grass samples through a 0.4 mm screen prior to analysis resulted in regressions with a larger RSD (± 3.3) than when a 1.0 mm screen was used to prepare the samples (P < 0.001).It was concluded that the digestibility in vivo of both legumes and grasses could be predicted by the pepsin-cellulase method by using the same equation, but to eliminate biased results, samples of known digestibility in vivo similar to those being tested should be included as standards in each study. The analytical time could be reduced by 48 h if shorter incubation periods (24 h) were used, but only at the expense of slightly larger errors in predicting DMD in vivo (P < 0.01). 相似文献
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P J McLeod 《CMAJ》1987,136(7):709-712
Twelve Canadian medical schools that had an organized faculty development program were surveyed to evaluate the extent to which such programs were used and to estimate their effectiveness. Common practices included sabbaticals and programs designed to improve instructional skills. The main problems included underfunding, poor participation and inadequate instructor evaluation. 相似文献
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The presentation involves students in a hands-on analysis of artifacts to create a time line of their local area's past societies. This exercise is more of a unit rather than a one-time activity. Procedures, ideas, and background for developing your own unit are presented in this article. The purpose of this unit is to engage students in applying the scientific method to archaeological research. Students will gain experience in designing and conducting their own investigations. 相似文献
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