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Mouaad Amine Mazri Reda Meziani 《Journal of plant biochemistry and biotechnology.》2013,22(2):176-184
We developed a novel large-scale micropropagation pathway for date palm (Phoenix dactylifera L.) based on organogenesis. We obtained organogenic stems from shoot tip explants of the Moroccan date palm cultivar Najda, and investigated shoot proliferation from these organogenic stems in vitro on various media; Beauchesne medium (BM) and Murashige and Skoog medium (MS) at full-strength, half-strength, and one-third-strength, containing various concentrations (0, 0.25, 0.5, and 1 mg/L) of 2-naphthoxyacetic acid (NOAA) and kinetin. The optimal medium during the multiplication phase was half-strength Murashige and Skoog medium (MS/2) supplemented with 0.5 mg/L NOAA and 0.5 mg/L kinetin (23.5 morphologically superior shoots per explant, with low vitrification rates). For the shoot elongation phase, shoots were transferred to the same proliferation medium, or to MS or MS/2 media without plant growth regulators (PGRs). Shoots elongated rapidly and showed a high rate of root formation on media supplemented with PGRs. For example, on MS/2 medium containing 1 mg/L NOAA and 1 mg/L kinetin, the average shoot length was 15.1 cm, the average number of roots per shoot was 6.2, and their average length was 3.4 cm. On PGR-free media, shoots were shorter with wider and greener leaves, and had fewer roots. The plantlets were transferred to a greenhouse for acclimation. The survival rate after 2 months was related to the medium used during the elongation phase; >90 % of shoots that were cultured on PGR-free media survived, while there was a poor survival rate of shoots that had been cultured on media containing PGRs. 相似文献
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Koufan Meriyem Mazri Mouaad Amine Essatte Amine Moussafir Sanae Belkoura Ilham El Rhaffari Lhoussaine Toufik Ibrahim 《Plant Cell, Tissue and Organ Culture》2020,140(2):369-378
Plant Cell, Tissue and Organ Culture (PCTOC) - European mistletoe (Viscum album) is a medicinal plant with significant anticancer properties. In vitro callus production provides an essential... 相似文献
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Koufan Meriyem Belkoura Ilham Mazri Mouaad Amine Amarraque Abderrahim Essatte Amine Elhorri Hafsa Zaddoug Fatima Alaoui Tajlmolk 《Plant Cell, Tissue and Organ Culture》2020,141(1):217-227
Plant Cell, Tissue and Organ Culture (PCTOC) - Argan (Argania spinosa (L.) Skeels) is an endangered and endemic agroforestry species of Morocco highly appreciated for its nutraceutical properties.... 相似文献
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Mazri Mouaad Amine Meziani Reda El Bakouri Zineb 《Plant Cell, Tissue and Organ Culture》2021,144(2):409-420
Plant Cell, Tissue and Organ Culture (PCTOC) - Dendrobium cariniferum Rchb. f. is an important crossing parent that is primarily used to produce aromatic Dendrobium orchid cultivars due to its... 相似文献
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Meziani Reda Mazri Mouaad Amine Essarioui Adil Alem Chakib Diria Ghizlane Gaboun Fatima El Idrissy Hicham Laaguidi Mohamed Jaiti Fatima 《Plant Cell, Tissue and Organ Culture》2019,137(2):285-295
Plant Cell, Tissue and Organ Culture (PCTOC) - We identified two strains of endophytic bacteria associated with date palm explants by 16S rRNA gene amplification and sequencing, and we explored... 相似文献
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