Induction of adventitious roots from leaf explants of Morinda coreia Buch. and ham. : an important dye yielding plant

Plant Cell, Tissue and Organ Culture (PCTOC) - The present communication reports, induction of adventitious roots (AR) from the in vitro raised leaves of Morinda coreia as affected by auxins and...     

<Emphasis Type="Italic">In vitro</Emphasis> propagation,micromorphological studies and <Emphasis Type="Italic">ex vitro</Emphasis> rooting of cannon ball tree (<Emphasis Type="Italic">Couroupita guianensis</Emphasis> aubl.): a multipurpose threatened species

In vitro propagation methods using seeds and nodal segments of a 21-year old Couroupita guianensis - a medicinally important but threatened tree have been developed. Hundred percent of the seeds germinated on half strength Murashige and Skoog (MS) medium with 2.0 mg l^?1 indole-3 butyric acid (IBA). Nodal segments were found most suitable for the establishment of cultures. About 90 % explants responded and 4.1 ± 0.23 shoots per node were induced after five weeks of inoculation on MS medium +4.0 mg l^?1 6-benzylaminopurine (BAP). Further shoot multiplication was achieved by repeated transfer of mother explants and subculturing of in vitro produced shoots on fresh medium. Maximum number (8.2 ± 0.17) of shoots were regenerated on MS medium with 1.0 mg l^?1 each of BAP and Kinetin (Kin) + 0.5 mg l^?1 α-naphthalene acetic acid (NAA) with additives (50 mg l^?1 of ascorbic acid and 25 mg l^?1 each of adenine sulphate, L-arginine and citric acid). The multiplied shoots rooted (4.3 ± 0.26 roots/shoot) on half strength MS medium with 2.5 mg l^?1 IBA. All the shoots were rooted ex vitro when pulse treated with 400 mg l^?1 of IBA for five min with an average of 7.3 ± 0.23 roots per shoot. Nearly 86 % of these plantlets were acclimatized within 7–8 weeks and successfully transferred in the field. Biologically significant developmental changes were observed during acclimation particularly in leaf micromorphology in terms of changes in stomata, veins and vein-islets, and trichomes. This study helps in understanding the response by the plants towards outer environmental conditions during acclimatization. This is the first report on micropropagation of C. guianensis, which could be used for the large-scale multiplication, restoration and conservation of germplasm of this threatened and medicinally important tree.     

Meta-topolin and liquid medium enhanced in vitro regeneration in Scaevola taccada (Gaertn.) Roxb

In Vitro Cellular & Developmental Biology - Plant - Scaevola taccada (Gaertn.) Roxb. is a hemi-sclerophyllous littoral shrub of the family Goodeniaceae. It is a salt-tolerant plant and used in...     

Mitigating in vitro induced macro- and micro-morphological anomalies using seismic stress in Vitex negundo L.

Plant Cell, Tissue and Organ Culture (PCTOC) - Present study explores the potential of seismo-mechanical stress in alleviating the macro- and micro-morphological aberrations observed in the in...     

Meta-topolin induced morphometric and structurally stable bulblets in Malabar River Lily (Amaryllidaceae)

The current investigation revealed a highly proficient and reproducible method for comprehensive in vitro regeneration and structural differentiation of bulblets in Crinum malabaricum Lekhak & Yadav using meta-topolin [mT; 6-(3-hydroxybenzylamino) purine] [6-benzylaminopurine] for the first time. Cultures were established from twin scales explants using optimized sterilization method and growth regulators for regeneration of in vitro plantlets. It was observed that mT at 2.0 mg L^?1 was the main factor involved in direct bulblets formation from the basal plate, and the highest mean number of bulblets per twin scale explant was 26 within 8 weeks. On maturation, the bulblets were rooted simultaneously on the mT containing medium. Regenerated plantlets were acclimatized with a 100% survival rate after 8 weeks. Light microscopic examination of bulblet scales/cataphylls was done to characterize the in vitro bulblets developed from mT and BAP containing media. The bulblet scales differed in quantitative traits of tissue systems, and the meta-topolin derived bulblets represented well differentiated dermal, ground and vascular tissues. The results obtained may provide a new tool to improve the production of pharmaceutically important compounds (viz. galanthamine) in bulblets and give the guarantee of survival of plants under natural conditions which can reaffirm the conservation measure of this critically endangered medicinal plant.

     

Correction to: Differential morphometric and micro-morpho-anatomical responses toward types of culture vessels used in micropropagation of Hemidesmus indicus (L.) R. Br.

Plant Cell, Tissue and Organ Culture (PCTOC) -     

Assessment of cell wall histochemistry of velamentous epiphytic roots in adaptive response of micropropagated plantlets of Vanda tessellata (Roxb.) Hook. ex G. Don

Plant Cell, Tissue and Organ Culture (PCTOC) - Velamentous roots of orchids are desiccation resistant and help in anchorage and absorption of water and nutrients. The specialized multi-layered,...     

Influence of meta-topolin on in vitro propagation and foliar micro-morpho-anatomical developments of Oxystelma esculentum (L.f.)Sm

Plant Cell, Tissue and Organ Culture (PCTOC) - Rooting is an important step of in vitro propagation that involves auxin signaling and the growth-promoting properties of phenolic compounds. We...     

Meta-topolin and liquid medium mediated enhanced micropropagation via ex vitro rooting in Vanilla planifolia Jacks. ex Andrews

Plant Cell, Tissue and Organ Culture (PCTOC) - An advanced micropropagation protocol has been developed for the global spice crop Vanilla planifolia using meta-topolin [mT, 6-(3-hydroxybenzylamino)...     

Morpho-anatomical and physiological changes of Indian sandalwood (Santalum album L.) plantlets in ex vitro conditions to support successful acclimatization for plant mass production

Santalum album L. (Indian sandalwood) is an economically important but vulnerable tropical tree species. Cultures were established via direct shoot regeneration from axillary buds on Murashige and Skoog (MS) medium supplemented with 2.5 mg L^?1 6-benzylaminopurine (BAP). The shoots were multiplied using MS medium containing 1.0 mg L^?1 BAP and 0.5 mg L^?1 indole-3 acetic acid and rooted on half strength MS medium containing 1.0 mg L^?1 indole-3 butyric acid. The rooted plantlets were hardened and acclimatized in greenhouse using soilrite® and cocopeat (1:1) mixture. The concentrations of photosynthetic pigments were analyzed and detected less under in vitro conditions (6.05 μg g^?1 FW) as compared to the 4 weeks old hardened (6.91 μg g^?1 FW) and 12 weeks old acclimatized plantlets (7.8 μg g^?1 FW) under greenhouse (ex vitro) environment. The anatomical evaluation of plantlets at subsequent stages of propagation suggested that the in vitro raised plantlets possessed structural abnormalities such as underdeveloped cuticle, unorganized tissue systems, reduced mesophyll tissues, fewer vascular elements and mechanical tissues, and loosely arranged thin walled paranchymatous ground tissues, which were slowly repaired during ex vitro hardening and acclimatization process to validate the developmental adaptation of micropropagated plantlets for maximum survival in the field (98.0% survival rate). The findings could help in the optimization of high-frequency commercial micropropagation of S. album for year-round production, and supply of this economically prominent vulnerable plant species to the farmers and the industries that rely on it.