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1.
Modeling of ethanol fermentation at high yeast concentrations 总被引:2,自引:0,他引:2
2.
The method of non-linear forecasting of time series was applied to different simulated signals and EEG in order to check its ability of distinguishing chaotic from noisy time series. The goodness of prediction was estimated, in terms of the correlation coefficient between forecasted and real time series, for non-linear and autoregressive (AR) methods. For the EEG signal both methods gave similar results. It seems that the EEG signal, in spite of its chaotic character, is well described by the AR model. 相似文献
3.
4.
Anonymous nuclear DNA markers in the American oyster and their implications for the heterozygote deficiency phenomenon in marine bivalves 总被引:4,自引:0,他引:4
A puzzling population-genetic phenomenon widely reported in allozyme
surveys of marine bivalves is the occurrence of heterozygote deficits
relative to Hardy-Weinberg expectations. Possible explanations for this
pattern are categorized with respect to whether the effects should be
confined to protein-level assays or are genomically pervasive and expected
to be registered in both protein- and DNA-level assays. Anonymous nuclear
DNA markers from the American oyster were employed to reexamine the
phenomenon. In assays based on the polymerase chain reaction (PCR), two
DNA-level processes were encountered that can lead to artifactual genotypic
scorings: (a) differential amplification of alleles at a target locus and
(b) amplification from multiple paralogous loci. We describe symptoms of
these complications and prescribe methods that should generally help to
ameliorate them. When artifactual scorings at two anonymous DNA loci in the
American oyster were corrected, Hardy-Weinberg deviations registered in
preliminary population assays decreased to nonsignificant values.
Implications of these findings for the heterozygote-deficit phenomenon in
marine bivalves, and for the general development and use of PCR-based
assays, are discussed.
相似文献
5.
Summary Aqueous feeds of 413 and 495 g/L glucose were fermented to ethanol at 90–95% conversion in a continuous flow extractive fermentation system with cell recycle. Compared to the continuous conventional fermentation of a 195 g/L glucose medium, the volumetric productivity was more than doubled in extractive mode, with no deleterious effects on cell viability, specific glucose consumption rate or ethanol yield. The use of an effective, biocompatible and stable in situ extractant with flash vaporization can also produce a concentrated ethanol vapour stream, reducing distillation costs of the product. 相似文献
6.
John J. Malinowski Bruce L. Grasberger Gary Trakshel Edward E. Huston Tracey M. Banks Patricia G. Brake Richard B. Ciccarelli Barry N. Jones James A. Koehn Diane Kratz Nicole Lundberg Panayiotis E. Stevis Carla T. Helaszek Mark A. Ator Angela M. Small Wood Travis Stams Byron Rubin Richard S. Alexander 《Protein science : a publication of the Protein Society》1995,4(10):2149-2155
7.
Basolateral plasma membrane localization of ouabain-sensitive sodium transport sites in the secretory epithelium of the avian salt gland 下载免费PDF全文
The distribution of Na+ pump sites (Na+-K+-ATPase) in the secretory epithelium of the avian salt gland was demonstrated by freeze-dry autoradiographic analysis of [(3)H] ouabain binding sites. Kinetic studies indicated that near saturation of tissue binding sites occurred when slices of salt glands from salt-stressed ducks were exposed to 2.2 μM ouabain (containing 5 μCi/ml [(3)H]ouabain) for 90 min. Washing with label-free Ringer's solution for 90 min extracted only 10% of the inhibitor, an amount which corresponded to ouabain present in the tissue spaces labeled by [(14)C]insulin. Increasing the KCl concentration of the incubation medium reduced the rate of ouabain binding but not the maximal amount bound. In contrast to the low level of ouabain binding to salt glands of ducks maintained on a freshwater regimen, exposure to a salt water diet led to a more than threefold increase in binding within 9-11 days. This increase paralleled the similar increment in Na+-K+-ATPase activity described previously. [(3)H]ouabain binding sites were localized autoradiographically to the folded basolateral plasma membrane of the principal secretory cells. The luminal surfaces of these cells were unlabeled. Mitotically active peripheral cells were also unlabeled. The cell-specific pattern of [(3)H]ouabain binding to principal secretory cells and the membrane-specific localization of binding sites to the nonluminal surfaces of these cells were identical to the distribution of Na+-K+-ATPase as reflected by the cytochemical localization of ouabain-sensitive and K+-dependent nitrophenyl phosphatase activity. The relationship between the nonluminal localization of Na+-K+-ATPase and the possible role of the enzyme n NaCl secretion is considered in the light of physiological data on electrolyte transport in salt glands and other secretory epithelia. 相似文献
8.
Douglas P. Malinowski Mary Gourley Susan Edelstein Robert E. Pearson 《Cell biochemistry and biophysics》1992,21(1-3):1-12
A single-chain antibody fragment has been constructed for an antibody that binds to theChlamydia specific carbohydrate structure of the lipopolysaccharide. Single-chain protein was expressed and secreted into the periplasmic
space ofE. coli as a fusion protein with the maltose binding protein. The fusion protein was purified in one step by virtue of its ability
to bind to maltose. In a sandwich ELISA, the eluted protein boundChlamydia lipopolysaccharide, which demonstrates that the single-chain protein domain will function as part of a fusion protein. The
expression of maltose binding fusion proteins into the periplasmic space could be used for production of other single-chain
antibodies or protein fragments requiring appropriate folding and disulfide bond formation. 相似文献
9.
A. B. Jarzębski J. J. Malinowski G. Goma P. Soucaille 《Bioprocess and biosystems engineering》1992,7(7):315-317
Simulations of continuous ethanol or acetonobutylic fermentations in aqueous two-phase systems show that at high substrate feed concentrations it is possible to obtain solvent productivities about 25–40% higher than in conventional systems with cell recycle if the biomass bleed rate is kept about one tenth of the value of D.List of Symbols
a
Volumetric fraction of dextran rich phase
-
B h–1
Bleed rate
-
D h–1
Dilution rate
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P kg m–3
Product concentration
-
PD kg m–3 h–1
Productivity
-
S kg m–3
Substrate
-
X kg m–3
Biomass
-
Partition coefficient 相似文献
10.
Edmund Malinowski 《Molecular & general genetics : MGG》1920,22(4):225-235
Ohne Zusammenfassung 相似文献