全文获取类型
收费全文 | 1913篇 |
免费 | 215篇 |
国内免费 | 1篇 |
出版年
2023年 | 7篇 |
2022年 | 7篇 |
2021年 | 48篇 |
2020年 | 36篇 |
2019年 | 46篇 |
2018年 | 49篇 |
2017年 | 38篇 |
2016年 | 63篇 |
2015年 | 100篇 |
2014年 | 97篇 |
2013年 | 104篇 |
2012年 | 132篇 |
2011年 | 130篇 |
2010年 | 76篇 |
2009年 | 103篇 |
2008年 | 110篇 |
2007年 | 91篇 |
2006年 | 97篇 |
2005年 | 87篇 |
2004年 | 79篇 |
2003年 | 77篇 |
2002年 | 63篇 |
2001年 | 40篇 |
2000年 | 21篇 |
1999年 | 32篇 |
1998年 | 24篇 |
1997年 | 10篇 |
1996年 | 16篇 |
1995年 | 14篇 |
1994年 | 18篇 |
1993年 | 9篇 |
1992年 | 23篇 |
1991年 | 25篇 |
1990年 | 27篇 |
1989年 | 22篇 |
1988年 | 11篇 |
1987年 | 10篇 |
1986年 | 17篇 |
1985年 | 10篇 |
1984年 | 13篇 |
1983年 | 12篇 |
1982年 | 8篇 |
1980年 | 7篇 |
1979年 | 11篇 |
1977年 | 8篇 |
1976年 | 8篇 |
1975年 | 7篇 |
1974年 | 8篇 |
1973年 | 11篇 |
1972年 | 15篇 |
排序方式: 共有2129条查询结果,搜索用时 15 毫秒
1.
Travis Park Felix G. Marx Erich M. G. Fitzgerald Alistair R. Evans 《Journal of morphology》2017,278(6):801-809
The pygmy right whale, Caperea marginata , is the least understood extant baleen whale (Cetacea, Mysticeti). Knowledge on its basic anatomy, ecology, and fossil record is limited, even though its singular position outside both balaenids (right whales) and balaenopteroids (rorquals + grey whales) gives Caperea a pivotal role in mysticete evolution. Recent investigations of the cetacean cochlea have provided new insights into sensory capabilities and phylogeny. Here, we extend this advance to Caperea by describing, for the first time, the inner ear of this enigmatic species. The cochlea is large and appears to be sensitive to low‐frequency sounds, but its hearing limit is relatively high. The presence of a well‐developed tympanal recess links Caperea with cetotheriids and balaenopteroids, rather than balaenids, contrary to the traditional morphological view of a close Caperea‐balaenid relationship. Nevertheless, a broader sample of the cetotheriid Herpetocetus demonstrates that the presence of a tympanal recess can be variable at the specific and possibly even the intraspecific level. 相似文献
2.
Maria Weinert Tharakeswari Selvakumar Travis S. Tierney Kambiz N. Alavian 《Journal of visualized experiments : JoVE》2015,(96)
Degeneration of mesencephalic dopaminergic (mesDA) neurons is the pathological hallmark of Parkinson’s diseae. Study of the biological processes involved in physiological functions and vulnerability and death of these neurons is imparative to understanding the underlying causes and unraveling the cure for this common neurodegenerative disorder. Primary cultures of mesDA neurons provide a tool for investigation of the molecular, biochemical and electrophysiological properties, in order to understand the development, long-term survival and degeneration of these neurons during the course of disease. Here we present a detailed method for the isolation, culturing and maintenance of midbrain dopaminergic neurons from E12.5 mouse (or E14.5 rat) embryos. Optimized cell culture conditions in this protocol result in presence of axonal and dendritic projections, synaptic connections and other neuronal morphological properties, which make the cultures suitable for study of the physiological, cell biological and molecular characteristics of this neuronal population. 相似文献
3.
4.
To determine how chromosome segregation is coordinated with nuclear envelope formation (NEF), we examined the dynamics of NEF in the presence of lagging acentric chromosomes in Drosophila neuroblasts. Acentric chromosomes often exhibit delayed but ultimately successful segregation and incorporation into daughter nuclei. However, it is unknown whether these late-segregating acentric fragments influence NEF to ensure their inclusion in daughter nuclei. Through live analysis, we show that acentric chromosomes induce highly localized delays in the reassembly of the nuclear envelope. These delays result in a gap in the nuclear envelope that facilitates the inclusion of lagging acentrics into telophase daughter nuclei. Localized delays of nuclear envelope reassembly require Aurora B kinase activity. In cells with reduced Aurora B activity, there is a decrease in the frequency of local nuclear envelope reassembly delays, resulting in an increase in the frequency of acentric-bearing, lamin-coated micronuclei. These studies reveal a novel role of Aurora B in maintaining genomic integrity by promoting the formation of a passageway in the nuclear envelope through which late-segregating acentric chromosomes enter the telophase daughter nucleus. 相似文献
5.
Tao Tan Jun Wu Chenyang Si Shaoxing Dai Youyue Zhang Nianqin Sun E Zhang Honglian Shao Wei Si Pengpeng Yang Hong Wang Zhenzhen Chen Ran Zhu Yu Kang Reyna Hernandez-Benitez Llanos Martinez Martinez Estrella Nuñez Delicado W. Travis Berggren Juan Carlos Izpisua Belmonte 《Cell》2021,184(8):2020-2032.e14
6.
While the life history traits of animals usually exhibit substantial phenotypic plasticity, such plasticity might reflect
either a simple alteration in the level of energy accrual and use or a genuine shift in energy allocation tactics between
environmental conditions. The latter would represent genuine plasticity in the life history itself, and thus it is important
to distinguish which of these two processes underlies the observed plasticity of life history traits. We investigated this
issue by examining the effects of temperature and salinity variation during ontogeny on the allocation of biomass and lipid
storage in male sailfin mollies, Poecilia latipinna. We raised males from four natural populations from birth to maturity in controlled laboratory conditions. Neither distinct
temperatures (23 or 29°C) nor different salinity regimes (2, 12, or 20 parts per thousand) affected body mass, although males
from different populations differed substantially in body mass. However, males raised at the higher temperature had a greater
allocation of biomass to testis and a lower allocation to viscera mass. The amount of stored lipid was altered by temperature
variation but the direction and magnitude of the effect varied substantially among males from the different populations. Salinity
variation affected neither biomass allocation nor the level of lipid storage. These results indicate that male mollies possess
a flexible developmental program with respect to temperature that canalizes body size and alters the allocation of biomass
among competing demands for reproductive readiness and capacity for energy storage.
Received: 25 November 1996 / Accepted: 1 December 1997 相似文献
7.
8.
Calvin A. Henard Travis J. Bourret Miryoung Song Andrés Vázquez-Torres 《The Journal of biological chemistry》2010,285(47):36785-36793
We report herein a critical role for the stringent response regulatory DnaK suppressor protein (DksA) in the coordination of antioxidant defenses. DksA helps fine-tune the expression of glutathione biosynthetic genes and discrete steps in the pentose phosphate pathway and tricarboxylic acid cycle that are associated with the generation of reducing power. Control of NAD(P)H/NAD(P)+ redox balance by DksA fuels downstream antioxidant enzymatic systems in nutritionally starving Salmonella. Conditional expression of the glucose-6-phosphate dehydrogenase-encoding gene zwf, shown here to be under DksA control, increases both the NADPH pool and antioxidant defenses of dksA mutant Salmonella. The DksA-mediated coordination of redox balance boosts the antioxidant defenses of stationary phase bacteria. Not only does DksA increase resistance of Salmonella against hydrogen peroxide (H2O2), but it also promotes fitness of this intracellular pathogen when exposed to oxyradicals produced by the NADPH phagocyte oxidase in an acute model of infection. Given the role of DksA in the adjustment of gene expression in most bacteria undergoing nutritional deprivation, our findings raise the possibility that the control of central metabolic pathways by this regulatory protein maintains redox homeostasis essential for antioxidant defenses in phylogenetically diverse bacterial species. 相似文献
9.
Myosin heavy chains are encoded by distinct members of a multigene family at different stages of muscle development. Study of the underlying regulatory mechanisms has been hindered because transitions in myosin expression have not been readily attained in tissue culture. Here we show a transition from early (fetal) to late (perinatal/adult) myosins defined by two monoclonal antibodies, F1.652 and N3.36, in the myotubes of mouse C2C12 cells. On day 1 of differentiation, essentially all myosin was early myosin. By day 8, early myosin dropped to 25% of its day 1 value and was replaced by late myosin. The transition occurred without neural contact, connective tissue components, or complex substrates, suggesting that its regulation may be intrinsic to the muscle cell. Our results demonstrate that a developmental progression in myosin gene expression, which occurs rapidly, with high frequency, and under relatively simple conditions, is now amenable to molecular analysis in cultured muscle cells. 相似文献
10.
Opportunities to investigate selection in free-living species during a naturally occurring epidemic are rare; however, we assessed innate immunocompetence in Florida scrub-jays before the population suffered the greatest over-winter mortality in 20 years of study. Propitiously, three months prior to the epidemic, we had sampled a number of male breeders to evaluate a suite of physiological measures that are commonly used to estimate the overall health-state of an individual. There was a significant, positive selection gradient for both Escherichia coli bacterial killing capability and body condition, suggesting that directional selection had occurred upon each of these traits during the disease epidemic. 相似文献