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1.
Omelchenko V. T. Salmenkova E. A. Makhotkin M. A. Romanov N. S. Altukhov Yu. P. Dudkin S. I. Dekhta V. A. Rubtsova G. A. Kovalev M. Yu. 《Russian Journal of Genetics》2004,40(8):910-918
The introduction of Far Eastern mullet (pilengas) in the Azov Sea in the 1970s–1980s has resulted in the formation of a self-reproducing commercial population. We have carried out a comparative population-genetic analysis of the mullet from the native (Primorye, the Sea of Japan basin) and the new (The Azov Sea basin) ranges. Genetic characteristics of three Primorye and three Azov local samples were studied using electrophoretic analysis of 15 enzymes encoded by 21 gene loci. In the Azov mullet, the initial heterozygosity characteristic of the donor population was preserved while the genotype and the allele compositions changed; the changes included a 1.9-fold reduction in the percentage of polymorphic loci and 1.5-fold reduction in the mean number of alleles per locus. The genetic differences between the Azov and the Primorye sample groups were highly significant. In the native range, no genetic differentiation among the mullet samples from different areas was found (G
st = 0.42%), whereas in the Azov Sea basin, the samples from spatially isolated populations (ecological groups) exhibited genetic differences (G
st = 1.38%). The genetic divergence of the subpopulations and the excess of heterozygotes at some loci in the Azov mullet suggest selection processes that formed genetically divergent groups associated with the areas of different salinity in the new range. The salinity level is assumed to be the most probable factor of local differentiating selection during fast adaptation and naturalization of the introduced mullet. 相似文献
2.
Distribution and Cytogenetic Features of Triploid Males of Crucian Carp in Azov Basin 总被引:1,自引:0,他引:1
Abramenko M. I. Nadtoka E. V. Makhotkin M. A. Kravchenko O. V. Poltavtseva T. G. 《Russian Journal of Developmental Biology》2004,35(5):305-315
When studying uni-bisexual crucian carp (Carassius auratus gibelio) populations in the Azov basin in 1995–2000, we found triploid males, which constituted 2.5%, on average, of the total numbers of studied samples. The areas of nuclei of erythrocytes of triploid males were, on average, 1.35 times those in diploid males. At the same optical density of DNA, the sizes of mature spermatozoon heads in triploid males were, on average, 1.8 times smaller than in diploid males, as follows from the data obtained in summer 1996. The results of similar studies carried out during the period of natural spawning activity in 1997–1999 suggest that the sizes of spermatozoon heads in triploid males were, on the contrary, 1.5 those in diploid males. Triploid males were characterized by mosaicism of spermatozoon sizes and chromosome mosaicism in somatic cells. Electrophoretic analysis for the locus of transferrin confirmed the triploid status of this genetic group. The results of comparative crosses of crucian carps with different ploidy suggest a high fertilizing capacity of triploid males, as well as normal viability of their progenies. A distinct positive correlation (r = 0.73) was found between the numbers of triploid females and triploid males in mixed di-triploid populations. No significant correlation was found between males and females within di- and triploid forms. 相似文献
3.
V. A. Tarasov M. A. Makhotkin N. V. Boyko E. F. Shin M. G. Tyutyakina I. E. Chikunov A. V. Naboka A. N. Mashkarina A. A. Kirpiy D. G. Matishov 《Russian Journal of Genetics》2017,53(5):551-560
We studied microRNA gene expression in HeLa cells following exposure for 6 h and 8 days to Co60 gamma rays at a dose of 4 Gy using an approach of large-scale parallel DNA sequencing. We identified 12 microRNAs with aberrant expression which were maintained in cell generations. The analysis of radiation-induced aberrant expression of pre-microRNAs made it possible to assess the importance of nuclear and cytoplasmic stages of microRNA biogenesis for preservation of its aberrant expression. On cell treatment by 5-azacytidine, aberrant expression was maintained only in two microRNAs: miR-21-3p and miR-422a, which demonstrated an increase in expression. Radiation-induced decrease in expression in ten examined microRNAs was dependent on DNA demethylation. At the same time, expression in a microRNA set, which demonstrated inheritable alteration of the expression after gamma-radiation exposure in the untreated cells, was not dependent or was weakly dependent on DNA methylation. The obtained results suggest that ionizing radiation induces aberrant DNA methylation, which affects inherited expression changes in microRNAs in cell generations after exposure to the mutagen. 相似文献
4.
V. A. Tarasov M. A. Makhotkin E. F. Shin N. V. Boiko M. G. Tyutyakina I. E. Chikunov A. V. Naboka A. N. Mashkarina A. A. Kirpii D. G. Matishov 《Doklady. Biochemistry and biophysics》2016,467(1):99-101
It was first shown that DNA damage induction in mitomycin C-treated HeLa cells leads to a change in the selection of 5p and 3p microRNA duplex strands in the formation of the RNA-induced silencing complex (RISC). 相似文献
5.
A. V. Semina N. E. Polyakova M. A. Makhotkin V. A. Brykov 《Russian Journal of Marine Biology》2007,33(3):187-192
Three Mugilid species: Mugil cephalus (Linnaeus, 1758) and Liza haematocheila (Temminck et Schlegel, 1845; syn. Mugil soiuy, M. haematocheilus, L. soiuy, Chelon haematocheilus) from the Sea of Japan, as well as M. cephalus and Liza aurata (Risso, 1810) from the Sea of Azov were investigated on the basis of PCR-RFLP analysis of mitochondrial DNA (mtDNA) fragments, which included 12S/16S rRNA, and ND3/ND4L/ND4 genes. Among 61 individuals of three Mugilid species thirteen different haplotypes were detected. Eight and thirteen restriction endonucleases were found to be species-specific in 12S/16SrRNA and ND3/ND4L/ND4 respectively. This method may be useful for species identification. M. cephalus showed the largest genetic divergence while L. haematocheila and L. aurata were closely related and clustered together. The level of mtDNA differentiation between the two M. cephalus samples from the Sea of Japan and the Sea of Azov, i.e., nucleotide substitutions of approximately 3%, appeared to be relatively high. 相似文献
6.
Omel'chenko VT Salmenkova EA Makhotkin MA Romanov NS Altukhov IuP Dudkin SI Dekhta VA Rubtsova GA Kovalev MIu 《Genetika》2004,40(8):1113-1122
The introduction of Far Eastern mullet (pilengas) in the Azov Sea in the 1970s-1980s has resulted in the formation of a self-reproducing commercial population. We have carried out a comparative population-genetic analysis of the mullet from the native (Primorye, the Sea of Japan basin) and the new (The Azov Sea basin) ranges. Genetic characteristics of three Primorye and three Azov local samples were studied using electrophoretic analysis of 15 enzymes encoded by 21 gene loci. In the Azov mullet, the initial heterozygosity characteristic of the donor population was preserved while the genotype and the allele compositions changed; the changes included a 1.9-fold reduction in the percentage of polymorphic loci and 1.5-fold reduction in the mean number of alleles per locus. The genetic differences between the Azov and the Primorye sample groups were highly significant. In the native range, no genetic differentiation among the mullet samples from different areas was found (Gst = 0.42%), whereas in the Azov Sea basin, the samples from spatially isolated populations (ecological groups) exhibited genetic differences (Gst = 1.38). The genetic divergence of the subpopulations and the excess of heterozygotes at some loci in the Azov mullet suggest selection processes that formed genetically divergent groups associated with the areas of different salinity in the new range. The salinity level is assumed to be the most probable factor of local differentiating selection during fast adaptation and naturalization of the introduced mullet. 相似文献
7.
Tarasov V. A. Naboka A. V. Makhotkin M. A. Chikunov I. E. Tyutyakina M. G. Chebotarev D. A. Cherkasova E. N. Kogan M. I. Chibichyan M. B. Matishov D. G. 《Russian Journal of Genetics》2019,55(6):720-727
Russian Journal of Genetics - A spectrum of differentially expressed microRNAs was determined by the massively parallel sequencing method in normal healthy prostate tissues, in hormone-dependent... 相似文献
8.
V. A. Tarasov N. V. Boyko M. A. Makhotkin E. F. Shin M. G. Tyutyakina I. E. Chikunov A. V. Naboka A. N. Mashkarina A. A. Kirpiy D. G. Matishov 《Russian Journal of Genetics》2016,52(11):1117-1123
The dependence of expression of miRNAs and their precursors (pre-miRNAs) on the DNA methylation level in HeLa cells 8 days after mitomycin C treatment was studied. A massive parallel DNA sequencing method was applied to analyze miRNA expression. 5-Azacytidine (DNA methylation inhibitor) was added to the medium 6 days after mutagenic agent exposure. The results indicated that the change in expression for some mature miRNAs (39 of 61) was accompanied by the change in the expression of their pre-miRNAs, while there were no significant changes in the expression of pre-miRNA for other mature miRNAs (22 of 61). The aberrant expression was maintained by 8 of 61 mature miRNAs and 6 of 55 pre-miRNAs in the induced HeLa cells after 5-azacytidine treatment. In addition, the expression of more than 90% of miRNAs, which indicated a significant change in expression after mitomycin C treatment, does not depend or depends slightly on the DNA methylation level in HeLa cells without mitomycin C treatment. The results suggest that mitomycin C induces aberrant DNA methylation which affects maintenance of changes in the miRNA expression in cell generations after mutagen treatment. 相似文献
9.
V. A. Tarasov M. G. Tyutyakina M. A. Makhotkin E. F. Shin A. V. Naboka A. N. Mashkarina D. A. Chebotarev E. N. Cherkasova M. I. Kogan M. B. Chibichyan D. G. Matishov 《Doklady. Biochemistry and biophysics》2018,479(1):101-104
Using multiple parallel sequencing on Illumina platform, we identified eight microRNAs that showed significant opposite changes of gene expression in cells of the hormone-sensitive LNCaP prostate cancer cell line and in cells of the hormone-resistant DU-145 cell line, in comparison to the microRNA expression in the normal prostate tissue cells. We found that the insulin-like growth factor 1 receptor (IGF1R) gene is a target of five microRNAs whose expression is increased in LNCaP cells and reduced in DU-145 cells. 相似文献
10.
V. A. Tarasov D. G. Matishov E. F. Shin N. V. Boyko N. N. Timoshkina M. A. Makhotkin A. M. Lomonosov A. A. Kirpiy O. I. Kit A. Yu. Maximov 《Russian Journal of Genetics》2014,50(10):1090-1101
Applying the method of multiple parallel sequencing on the MiSeq platform (Illumina, United States), a comparative analysis of miRNA expression in tumor and normal colon tissue cells was performed. Forty miRNAs aberrantly expressed in cancer were detected. Among them, 15 and 25 miRNAs showed increased and decreased expression, respectively, for all or most of the cases. Sixteen miRNA clusters were identified, which showed a coordinated or incompletely coordinated aberrant expression in colorectal cancer cells. In two (miR-183/182 and miR-106b/25) and four (miR-143/145, miR-497/195, miR-30e/30c-1, and miR-30a/30c-2) miRNA clusters, respectively, a statistically significant coordinated increase or decrease in expression was registered for all miRNAs within the corresponding cluster. Three aberrantly expressed well-known miRNAs (miR-100-5p, miR-30d-5p, and miR-204-5p) were identified, which, however, had never before been associated with colorectal cancer. The obtained results demonstrate the potential and promising application of 6 miRNA clusters with coordinated aberrant expression as markers for colorectal cancer. 相似文献