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1.
Esposito Francesco Memoli Valeria Panico Speranza Claudia Trifuoggi Marco Di Natale Gabriella Maisto Giulia 《Plant and Soil》2019,436(1-2):517-526
Plant and Soil - The aims of this research were: i) to compare Cr, Cu, Ni and Pb concentrations in Quercus ilex L. leaves collected at urban/industrial and urban areas; ii) to investigate the main... 相似文献
2.
3,4-dihydroxyphenylethanol (hydroxytyrosol; DPE) is the major phenolic antioxidant present in extra virgin olive oil, either in a free or esterified form. Despite its relevant biological effects, no data are available on its bioavailability and metabolism. The aim of the present study is to examine the molecular mechanism of DPE intestinal transport, using differentiated Caco-2 cell monolayers as the model system. The kinetic data demonstrate that [(14)C]DPE transport occurs via a passive diffusion mechanism and is bidirectional; the calculated apparent permeability coefficient indicates that the molecule is quantitatively absorbed at the intestinal level. The only labelled DPE metabolite detectable in the culture medium by HPLC (10% conversion) is 3-hydroxy-4-methoxyphenylethanol, the product of catechol-O-methyltransferase; when DPE is assayed in vitro with the purified enzyme a K(m) value of 40 microM has been calculated. 相似文献
3.
Flow cytometry is a widely recognized method of rapidly assessing the ploidy and proliferation status of experimental and solid tumors. In the present work, a variety of human cancers from various sites (lung, head and neck, etc.), of traditional interest in our laboratory, have been analyzed. In agreement with a number of recent reports, a general heterogeneity of human solid tumors can be evidenced. In particular: (a) solid tumors are characterized by a variable degree of aneuploidy; (b) the internal structure of solid tumors is highly heterogeneous especially with respect to the fraction of aneuploid malignant cells and their distribution through the cycle phases; and (c) some solid tumors are also characterized by the presence, to a variable extent, in the tumor of mass of multiple cell clones. Static fluorimetry of Feulgen-stained (mitotic) single cells offers a way to confirm this kind of observations. 相似文献
4.
A. Alfani G. Bartoli F. A. Rutigliano G. Maisto A. Virzo De Santo 《Biological trace element research》1996,51(1):117-131
The concentrations of Pb, Cu, Fe, and Mn were analyzed in surface deposit and tissue ofQuercus ilex leaves from several sites of the urban area of Naples, exposed to different degrees of air pollution. These included some
major roads with heavy traffic loads, squares, and three urban parks. The soil from the trunk base area ofQ. ilex trees in the same sites was also analyzed for total and available metal contents. Pb, Cu, and Fe contents in the surface
deposit and leaf tissue were significantly higher (p<0.01) in leaves from roadside sites than in leaves from parks; significant correlations were found between deposit- and tissue-contents
of Pb, Cu, and Fe. Mn content in leaves from roadside sites and in leaves from parks were similar and Mn content in the leaf
deposit was irrelevant. Significant differences (p<0.001) in both total and available Pb and Cu soil content were found between sampling sites. Also for available Fe and Mn
soil content differences among sites were relevant, although the highest values were measured in soil from urban parks. A
positive correlation between leaf and soil metal content was found only for Pb, thus suggesting that trace metal contents
of leaves directly depend on atmospheric depositions. Seasonal variations of Pb, Cu, and Fe were pronounced at a polluted
site, whereas no relevant seasonal variation was observed at a control site; moreover, metal accumulation was high at the
polluted site. Mn content and seasonal dynamics were comparable at control and polluted sites. 相似文献
5.
Anna De Marco Angela Meola Giulia Maisto Maria Giordano Amalia Virzo De Santo 《Plant and Soil》2011,344(1-2):305-317
Many studies across a range of ecosystems have shown that decomposition in mixed litter is not predictable from single-species results due to synergistic or antagonistic interactions. Some studies also reveal that species composition and relative abundance may be more important than just richness in driving non-additive effects. Most studies on litter decomposition in Mediterranean maquis, an high-diversity shrubby ecosystem, have dealt exclusively with single species. In this study we investigated, at the individual-litter level, as well as at the litter-mixture level, the effect of litter mixing on decomposition of 3-species litter assemblages with different relative abundance of the component litters; we set up two types of litter assemblages that reflected the heterogeneity of bush cover in the inner maquis and at the edge maquis/gaps, as related to the leaf traits, i.e. sclerophylly vs mesophylly. We measured mass loss, decay of lignin, cellulose and ADSS (acid detergent soluble substances) and fungal mycelium ingrowth. The results show that over a 403-day incubation period, the decomposition of individual litters in mixtures deviated from that of monospecific litters and had different directions. In litter mixtures of the sclerophylls Phillyrea angustifolia and Pistacea lentiscus with the mesophyll Cistus, decomposition was lower than expected (antagonistic effect); in the mixtures of litters with similar physical structure (Ph. angustifolia and P. lentiscus with Quercus ilex) decomposition was faster than expected (synergistic effect). When considering the different decomposition phases, both negative and positive effects occurred in Quercus mixtures depending on the phase of decomposition. In both types of 3-species litter assemblages the greatest effect occurred in uneven mixtures rather than in even mixtures. Our results show that species composition drives the direction whilst the decomposability and the relative abundance drive the magnitude of non-additive effects of litter mixing on decomposition. 相似文献
6.
Maria Consiglia Trotta Gorizio Pieretti Francesco Petrillo Nicola Alessio Anca Hermenean Rosa Maisto Michele D'Amico 《Journal of cellular physiology》2020,235(5):4256-4267
No study has investigated the interaction of Resolvin D1 (RvD1) with mitochondrial damage of retinal cells caused by diabetes. This study aims to investigate the effects of RvD1 (50 nM) on morphological and biochemical indicators of mitochondrial damage in primary retinal cells exposed to 30 mM d -glucose high glucose (HG). HG-cells exhibited photoreceptor damage characterized by short and small mitochondria with prevalent mitochondrial disruption, fragmentation, and aggregation. The cells had low mitochondrial transporters TIMM44 and TOMM40, Connexin 43, NAD/NADH ratio, and ATP levels, whereas increased cytosolic cytochrome c. Moreover, they expressed high cytosolic metalloproteinase matrix metallopeptidase 9 (MMP-9) and MMP-2 activity. HG-cells treated with RvD1 (50 nM) showed reduced reactive oxygen species levels, improved mitochondrial morphology and function, promoted mitochondrial DNA repair by OGG1, and reduced cell apoptosis and metalloproteinase activity. Therefore, RvD1 induces protection from high glucose-load to the retinal cell and promotes their survival by decreasing cytosolic MMP and mitochondrial damage. 相似文献
7.
Claudio Bucolo Filippo Drago Rosa Maisto Giovanni Luca Romano Velia D'Agata Grazia Maugeri Salvatore Giunta 《Journal of cellular physiology》2019,234(10):17295-17304
To study the effects of curcumin on human retinal pigment epithelial (RPE) cells exposed to high glucose (HG) insult, we performed in vitro studies on RPE cells cultured both in normal and HG conditions to assess the effects of curcumin on the cell viability, nuclear factor erythroid 2-related factor 2 (Nrf2) expression, HO-1 activity, and ERK1/2 expression. RPE cells exposed to HG insult were treated with curcumin. The cell viability, apoptosis, HO-1 activity, ERK, and Nrf2 expression were evaluated. The data indicated that treatment with curcumin caused a significant decrease in terms of apoptosis. Further, curcumin was able to induce HO-1 expression via Nrf2 activation and counteracts the damage elicited by HG. The present study demonstrated that curcumin provides protection against HG-induced damage in RPE cells through the activation of Nrf2/HO-1 signaling that involves the ERK pathway, suggesting that curcumin may have therapeutic value in the treatment of diabetic retinopathy. 相似文献
8.
Ghorbel Sofiane Bkhairia Intidhar Dhibi Sabah Maisto Maria Alghamdi Othman A. Nasri Moncef Tenore Gian Carlo 《International journal of peptide research and therapeutics》2021,27(3):2069-2082
International Journal of Peptide Research and Therapeutics - The present study investigates the nephroprotective and the molecular mechanisms effects of Liza aurata protein hydrolysates (LAPHs)... 相似文献
9.
Causal mediation analyses with rank preserving models 总被引:2,自引:0,他引:2
We present a linear rank preserving model (RPM) approach for analyzing mediation of a randomized baseline intervention's effect on a univariate follow-up outcome. Unlike standard mediation analyses, our approach does not assume that the mediating factor is also randomly assigned to individuals in addition to the randomized baseline intervention (i.e., sequential ignorability), but does make several structural interaction assumptions that currently are untestable. The G-estimation procedure for the proposed RPM represents an extension of the work on direct effects of randomized intervention effects for survival outcomes by Robins and Greenland (1994, Journal of the American Statistical Association 89, 737-749) and on intervention non-adherence by Ten Have et al. (2004, Journal of the American Statistical Association 99, 8-16). Simulations show good estimation and confidence interval performance by the proposed RPM approach under unmeasured confounding relative to the standard mediation approach, but poor performance under departures from the structural interaction assumptions. The trade-off between these assumptions is evaluated in the context of two suicide/depression intervention studies. 相似文献