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ILARIA ZOLLINO DIANA CAMPIONI MARIA GRAZIA SIBILLA MIRKO TESSARI ANNA MARIA MALAGONI PAOLO ZAMBONI 《Cytotherapy》2019,21(2):200-211
Background aims
Preclinical and observational reports indicate that adipose tissue (AT) is a safe and promising tool to treat non-healing venous leg ulcers (VLUs).Methods
From an initial cohort of 38 patients, 16 patients affected by non-healing VLUs were randomly allocated to the experimental arm (5 men and 3 women) and control arm (5 men and 3 women). In the experimental arm, wounds were treated by debridement, centrifuged adipose tissue (CAT), advanced dressings and compression. No experimental treatment (CAT) was administered to the control arm. We investigated the functional and the immunophenotypical features of the harvested CAT-derived stem cells. The primary outcome measures were healing time and safety of the cell treatment. Secondary outcomes were pain evaluated by numeric rating scale (NRS), complete wound healing at 24 weeks by Margolis Index and wound-healing process expressed in square centimeters per week. The various immunophenotypic and functional characteristics of CAT-derived stem cells were then correlated with the clinical outcomes.Results
No major adverse events were recorded. The healing time was significantly faster by applying CAT, 17.5 ± 7.0 weeks versus 24.5 ± 4.9 weeks recorded in the control arm (P < 0.036). NRS dropped after the first week to 2.7 ± 2.0 in the experimental arm versus 6.6 ± 3.0 in the control group (P < 0.01). The rate of healing at the 24th week was not significantly different between arms. Interestingly, we found a strong reverse correlation between the percent of CD34+/CD45– non-hematopoietic cells, respectively, with the healing time (r?=?–0.894, P < 0.041) and NRS (r?=?–0.934, P < 0.020).Conclusions
CAT is safe and may accelerate healing time in VLUs as well as reduce wound pain. The percentage of CD34+/CD45– cells in stromal vascular fraction (SVF) seems to be a predictive biomarker of successful CAT treatment in these patients. 相似文献2.
ALESSANDRO GRAPPUTO FEDERICA BARBISAN MIRKO DE GIROLAMO ANDREA PILASTRO LORENZO ZANE 《Molecular ecology resources》2006,6(4):1070-1072
Eleven polymorphic microsatellite loci were isolated in the passeriform Petronia petronia using the enrichment protocol FIASCO (fast isolation by AFLP of sequences containing repeats). We detected three to 13 alleles per locus in 25 specimens collected from an Italian population. The level of expected heterozygosity ranged from 0.439 to 0.856. One locus is sex linked to the Z chromosome. The total exclusionary probabilities using these loci for the first and the second parent were 0.978 and 0.999, respectively. These are the first microsatellite loci characterized from the rock sparrow that can be used for estimating population structure and for large‐scale parentage analysis. 相似文献
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ABSTRACT In this paper a new method for the automatic classification of bird sounds is presented. Our method is based on acoustic parameters (features) taken from the first harmonic component computed from the sound spectrogram. The features are based on a line segment approximation of the first harmonic component. The final feature vectors, consisting of 16 real numbers, are then classified using a self-organizing map (SOM) neural network. Flight calls of four crossbill species (Loxia spp.) are used as a test example. In the first phase, an unsupervised network was trained and tested using common crossbill L. curvirostra flight calls recorded mainly in the Netherlands. The network was tested using two-barred L. leucoptera, Scottish L. scotica and parrot L. pytyopsittacus crossbill flight calls in the second phase. Finally, the results were validated applying the same network to flight calls of common crossbills and parrot crossbills recorded in Finland. The method automatically separated common crossbill flight calls from those of parrot crossbills. The classification accuracy of the Dutch recordings was 58% in the first phase and 54% in the second phase. The Finnish recordings were classified with 54% accuracy. 相似文献
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