全文获取类型
收费全文 | 4689篇 |
免费 | 420篇 |
国内免费 | 6篇 |
出版年
2023年 | 33篇 |
2022年 | 21篇 |
2021年 | 162篇 |
2020年 | 83篇 |
2019年 | 95篇 |
2018年 | 104篇 |
2017年 | 80篇 |
2016年 | 163篇 |
2015年 | 255篇 |
2014年 | 272篇 |
2013年 | 289篇 |
2012年 | 381篇 |
2011年 | 351篇 |
2010年 | 221篇 |
2009年 | 187篇 |
2008年 | 248篇 |
2007年 | 221篇 |
2006年 | 220篇 |
2005年 | 207篇 |
2004年 | 193篇 |
2003年 | 153篇 |
2002年 | 140篇 |
2001年 | 101篇 |
2000年 | 90篇 |
1999年 | 69篇 |
1998年 | 42篇 |
1997年 | 31篇 |
1996年 | 26篇 |
1995年 | 18篇 |
1994年 | 28篇 |
1993年 | 14篇 |
1992年 | 40篇 |
1991年 | 51篇 |
1990年 | 29篇 |
1989年 | 45篇 |
1988年 | 38篇 |
1987年 | 52篇 |
1986年 | 34篇 |
1985年 | 40篇 |
1984年 | 30篇 |
1983年 | 21篇 |
1982年 | 19篇 |
1981年 | 14篇 |
1980年 | 12篇 |
1979年 | 24篇 |
1978年 | 18篇 |
1977年 | 18篇 |
1975年 | 12篇 |
1974年 | 16篇 |
1973年 | 11篇 |
排序方式: 共有5115条查询结果,搜索用时 15 毫秒
1.
Four myeloid cell lines (M1, WEHI-3B D+, FDC-P1, and 32D) were screened for the presence of J11d antigen. One of these cell lines, the myeloid leukemia M1, was found to express a high level of J11d antigen on the cell surface. Recombinant mouse leukemic inhibitory factor (rm-LIF), recombinant human LIF (rh-LIF), and steroids (hydrocortisone and dexamethasone) could induce M1 cells to undergo monocytic differentiation. The level of J11d antigen was greatly reduced after treatment of the cells with LIF or steroids. Western blotting revealed that the apparent molecular weight of the J11d antigen on M1 cells was 45-48 kDa. Furthermore, the level of J11d mRNA was also reduced during LIF-induced differentiation of M1 cells. 相似文献
2.
3.
Luke L. M. Heaton Eduardo López Philip K. Maini Mark D. Fricker Nick S. Jones 《Proceedings. Biological sciences / The Royal Society》2010,277(1698):3265-3274
Cord-forming fungi form extensive networks that continuously adapt to maintain an efficient transport system. As osmotically driven water uptake is often distal from the tips, and aqueous fluids are incompressible, we propose that growth induces mass flows across the mycelium, whether or not there are intrahyphal concentration gradients. We imaged the temporal evolution of networks formed by Phanerochaete velutina, and at each stage calculated the unique set of currents that account for the observed changes in cord volume, while minimizing the work required to overcome viscous drag. Predicted speeds were in reasonable agreement with experimental data, and the pressure gradients needed to produce these flows are small. Furthermore, cords that were predicted to carry fast-moving or large currents were significantly more likely to increase in size than cords with slow-moving or small currents. The incompressibility of the fluids within fungi means there is a rapid global response to local fluid movements. Hence velocity of fluid flow is a local signal that conveys quasi-global information about the role of a cord within the mycelium. We suggest that fluid incompressibility and the coupling of growth and mass flow are critical physical features that enable the development of efficient, adaptive biological transport networks. 相似文献
4.
Enterochelin synthetase activity is controlled by both repression and feed-back inhibition mechanisms. Inclusion of iron in growth media results in synthesis of all four (D, E, F and G) components of enterochelin synthetase being repressed. The specific inhibition of L-serine activation (partial reaction catalyzed by the F component) by the end products, ferric-enterochelin and 2,3-dihydroxybenzoylserine, is shown to inhibit overall enterochelin synthetase activity. 相似文献
5.
6.
7.
Amy Chapman Gonzalo Gonzales Wayne R. Burrowes Paul Assanah Bernadette Iannone Michael K. Leung George B. Stefano 《Cellular and molecular neurobiology》1984,4(2):143-155
In Mytilus and Leucophaea the high-affinity binding site density is significantly lower in old animals than in young animals, whereas the low-affinity site density remains unchanged. In Mytilus the estimated met-enkephalin and met-enkephalin-Arg6-Phe7 levels are significantly higher in old than in young animals. In Leucophaea only the met-enkephalin level can be determined, and it is also higher in old animals. The decrease in the high-affinity binding site density and the corresponding increase in endogenous enkephalin levels suggest the existence of an opioid compensatory mechanism associated with the aging process. In Mytilus there is a demonstrated decrease with age in intraganglionic dopamine levels in response to applied opiates. In addition, the inhibition of dopamine-stimulated adenylate cyclase activity by opiates also decreases in older animals. In Leucophaea the sex difference in opioid binding densities diminishes with age. 相似文献
8.
9.
G A Dunaway G L Leung J R Thrasher M D Cooper 《The Journal of biological chemistry》1978,253(20):7460-7463
Earlier work demonstrated that the activity of liver phosphofructokinase (PFK-L2) and immunoreactive PFK-L2 were decreased in diabetic rats and increased to normal or super-normal amounts following insulin treatment (Dunaway, G.A., and Weber, G., (1974) Arch. Biochem. Biophys. 162, 629-637). This report indicates that the decrease in levels of PFK-L2 in diabetic rats is a result of an accelerated degradation rate while the synthetic rate remains nearly normal. Following insulin treatment, the rate of PFK-L2 synthesis is enhanced 2-fold, whereas the rate of degradation appears to be greatly diminished. An inverse relationship is shown to exist between the PFK-L2 levels and the rates of PFK-L2 degradation, suggesting that the levels of PFK-L2 are primarily regulated by degradation rate. In addition, the levels of the PFK-L2 peptide stabilizing factor are inversely proportional to rates of PFK-L2 degradation. These results indicate that insulin mediates the rate of degradation of PFK-L2 by controlling the level of the peptide stabilizing factor. 相似文献
10.