全文获取类型
收费全文 | 3774篇 |
免费 | 262篇 |
国内免费 | 1篇 |
出版年
2023年 | 24篇 |
2022年 | 14篇 |
2021年 | 78篇 |
2020年 | 46篇 |
2019年 | 62篇 |
2018年 | 104篇 |
2017年 | 62篇 |
2016年 | 115篇 |
2015年 | 180篇 |
2014年 | 205篇 |
2013年 | 284篇 |
2012年 | 334篇 |
2011年 | 282篇 |
2010年 | 205篇 |
2009年 | 167篇 |
2008年 | 245篇 |
2007年 | 215篇 |
2006年 | 203篇 |
2005年 | 184篇 |
2004年 | 158篇 |
2003年 | 169篇 |
2002年 | 180篇 |
2001年 | 48篇 |
2000年 | 31篇 |
1999年 | 31篇 |
1998年 | 33篇 |
1997年 | 21篇 |
1996年 | 33篇 |
1995年 | 23篇 |
1994年 | 36篇 |
1993年 | 24篇 |
1992年 | 22篇 |
1991年 | 26篇 |
1990年 | 16篇 |
1989年 | 12篇 |
1988年 | 12篇 |
1987年 | 17篇 |
1986年 | 10篇 |
1985年 | 18篇 |
1984年 | 19篇 |
1983年 | 15篇 |
1982年 | 7篇 |
1980年 | 7篇 |
1979年 | 8篇 |
1978年 | 9篇 |
1977年 | 4篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1971年 | 4篇 |
1967年 | 4篇 |
排序方式: 共有4037条查询结果,搜索用时 180 毫秒
1.
Using filtration enrichment techniques, an Aspergillus terreus arginine auxotrophic strain which contains a mutation that abolishes ornithine transcarbamylase (OTCase) activity has been isolated. This mutant has been genetically transformed with the cloned Aspergillus nidulans OTCase gene. Prototrophic transformants arose at a frequency of about 50 transformants per microgram of plasmid DNA. Southern blot analysis of DNA from the transformants showed that the transforming DNA was ectopically integrated at different locations in the A. terreus genome, often in multiple tandem copies. The transformants were phenotypically stable for several mitotic divisions and retained their capacity to produce extracellular enzymes. 相似文献
2.
3.
4.
Legionella pneumophila in Cooling Towers: Fluctuations in Counts, Determination of Genetic Variability by Pulsed-Field Gel Electrophoresis (PFGE), and Persistence of PFGE Patterns 下载免费PDF全文
Sonia Ragull Marian Garcia-Nuez Maria Luisa Pedro-Botet Nieves Sopena Maria Esteve Rafael Montenegro Miquel Sabri 《Applied microbiology》2007,73(16):5382-5384
The concentrations of Legionella pneumophila in cooling towers may vary considerably over short periods of time, producing significant fluctuations throughout the year. Despite genetic variability, in small geographical areas the same indistinguishable pulsed-field gel electrophoresis patterns may be shared among different cooling towers and persist over time. 相似文献
5.
6.
The goal of this study was to develop a new implantable transducer for measuring anterior cruciate ligament (ACL) graft tension postoperatively in patients who have undergone ACL reconstructive surgery. A unique approach was taken of integrating the transducer into a femoral fixation device. To devise a practical in vivo calibration protocol for the fixation device transducer (FDT), several hypotheses were investigated: (1) The use of a cable versus the actual graft as the means for applying load to the FDT during calibration has no significant effect on the accuracy of the FDT tension measurements; (2) the number of flexion angles at which the device is calibrated has no significant effect on the accuracy of the FDT measurements; (3) the friction between the graft and femoral tunnel has no significant effect on measurement accuracy. To provide data for testing these hypotheses, the FDT was first calibrated with both a cable and a graft over the full range of flexion. Then graft tension was measured simultaneously with both the FDT on the femoral side and load cells, which were connected to the graft on the tibial side, as five cadaver knees were loaded externally. Measurements were made with both standard and overdrilled tunnels. The error in the FDT tension measurements was the difference between the graft tension measured by the FDT and the load cells. Results of the statistical analyses showed that neither the means of applying the calibration load, the number of flexion angles used for calibration, nor the tunnel size had a significant effect on the accuracy of the FDT. Thus a cable may be used instead of the graft to transmit loads to the FDT during calibration, thus simplifying the procedure. Accurate calibration requires data from just three flexion angles of 0, 45, and 90 deg and a curve fit to obtain a calibration curve over a continuous range of flexion within the limits of this angle group. Since friction did not adversely affect the measurement accuracy of the FDT, the femoral tunnel can be drilled to match the diameter of the graft and does not need to be overdrilled. Following these procedures, the error in measuring graft tension with the FDT averages less than 10 percent relative to a full-scale load of 257 N. 相似文献
7.
Marcello Ventura Barbara Rieck Francesca Boldrin Giulia Degiacomi Marco Bellinzoni Nathalie Barilone Faisal Alzaidi Pedro M. Alzari Riccardo Manganelli Helen M. O'Hare 《Molecular microbiology》2013,90(2):356-366
Alpha‐ketoglutarate is a key metabolic intermediate at the crossroads of carbon and nitrogen metabolism, whose fate is tightly regulated. In mycobacteria the protein GarA regulates the tricarboxylic acid cycle and glutamate synthesis by direct binding and regulation of three enzymes that use α‐ketoglutarate. GarA, in turn, is thought to be regulated via phosphorylation by protein kinase G and other kinases. We have investigated the requirement for GarA for metabolic regulation during growth in vitro and in macrophages. GarA was found to be essential to Mycobacterium tuberculosis, but dispensable in non‐pathogenic Mycobacterium smegmatis. Disruption of garA caused a distinctive, nutrient‐dependent phenotype, fitting with its proposed role in regulating glutamate metabolism. The data underline the importance of the TCA cycle and the balance with glutamate synthesis in M. tuberculosis and reveal vulnerability to disruption of these pathways. 相似文献
8.
9.
10.
M L Zeneroli M Baraldi E Ventura C Vezzelli O Tofanetti M Germini I Casciarri 《Life sciences》1991,48(1):37-50
The binding characteristics of gamma-aminobutyric acid-A (GABA-A) receptors and the kinetic characteristics of the target enzyme of GABA synthesis in nerve terminals, glutamic acid decarboxylase (GAD), were studied in a dog model of portal-systemic encephalopathy obtained by porta-caval shunt performed in dimethylnitrosamine pretreated animals. Furthermore the properties of dopamine receptors and the levels of catecholamines of encephalopathic dogs were investigated. The mild stage of encephalopathy was characterized by an up-regulation of the inhibitory GABA-A receptors probably related to a decrese of GABA in nerve terminals since GAD was decreased and by a slight decrease of catecholamines and by an increased synthesis of octopamine associated with a decreased affinity of dopamine receptors. In the severe stage there was a selection of high affinity GABA-A receptors with an increased number of benzodiazepine recognition sites which were supersensitive to GABA stimulation, a decreased number of Dopamine D-2 receptors and a marked reduction of catecholamines. These data seem to suggest that the neurological disturbances of experimental portal-systemic encephalopathy might be the result of an imbalance between inhibitory and excitatory systems leading to a prevalence of the first one. 相似文献