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1.
Lech Michalczuk Robert S. Bandurski 《Biochemical and biophysical research communications》1980,93(2):588-592
We have demonstrated the in vitro enzymatic synthesis of an ester of indole-3-acetic acid (IAA) and glucose and of IAA and myo-inositol by the following reaction sequence: lt]o| li]1) IAA + UDPG ? IAA-glucose +UDP li]2) IAA-glucose +myo-inositol → IAA-itmyo-inositol +glucose The enzymes were partially purified from extracts of immature kernels of Zea mays sweet corn and the two activities separated on a Sephadex G-150 column. Products were characterized, primarily, by comparison of their 70 eV mass spectra with those of authentic synthetic standards. To our knowledge this is the first example of enzymatically catalyzed acylation by a 1-O-acylsugar. 相似文献
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B?a?ej Po?niak Tomasz Grabowski Karolina Motykiewicz-Pers Kamila Bobrek Lech Rak Katarzyna Bobusia Andrzej Gawe? Marcin ?wita?a 《PloS one》2015,10(4)
Salicylates were the first non-steroid anti-inflammatory drugs (NSAIDs) to be used in any species and are still widely used in humans and livestock. However, the data on their pharmacokinetics in animals is limited, especially after repeated administration. Evidence exist that in chickens (Gallus gallus) salicylate (SA) may induce its own elimination. The aim of this study was to investigate salicylate pharmacokinetics and egg residues during repeated administration of sodium salicylate (SS) to laying hens. Pharmacokinetics of SA was assessed during 14 d oral administration of SS at daily doses of 50 mg/kg and 200 mg/kg body weight to laying hens. On the 1st, 7th and 14th d a 24 h-long pharmacokinetic study was carried out, whereas eggs were collected daily. Salicylate concentrations in plasma and eggs were determined using high-performance liquid chromatography with ultraviolet detection and pharmacokinetic variables were calculated using a non-compartmental model. Mean residence time (MRT), minimal plasma concentration (Cmin, C16h) and elimination half-life (T1/2el) of SA showed gradual decrease in layers administered with a lower dose. Total body clearance (ClB) increased. Layers administered with the higher dose showed a decrease only in the T1/2el. In the low dose group, SA was found only in the egg white and was low throughout the experiment. Egg whites from the higher dose group showed initially high SA levels which significantly decreased during the experiment. Yolk SA levels were lower and showed longer periods of accumulation and elimination. Repeated administration of SS induces SA elimination, although this effect may differ depending on the dose and production type of a chicken. Decreased plasma drug concentration may have clinical implications during prolonged SS treatment. 相似文献
4.
A new tortoise beetle species, Cyrtonota
abrili, is described from the Antioquia and Caldas departments in Colombia. New faunistic data are provided for 87 species, including 16 new additions to the country’s fauna. A checklist of the known 238 species of tortoise beetles recorded from Colombia is given. 相似文献
5.
Anna Sankiewicz Piotr Laudanski Lech Romanowicz Adam Hermanowicz Wiesława Roszkowska-Jakimiec Wojciech Debek Ewa Gorodkiewicz 《Analytical biochemistry》2015
We have developed a new method for highly selective determination of the ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) concentration using a surface plasmon resonance imaging (SPRI) technique and two different biosensors. UCH-L1 was captured from a solution by immobilized specific rabbit monoclonal antibody or specific LDN-57444 inhibitor due to formation of receptor–UCH-L1 complex on the biosensor surface. The analytically useful dynamic response range of both biosensors is between 0.1 and 2.5 ng/ml. The detection limit is 0.06 ng/ml for the biosensor with antibody and 0.08 ng/ml for the biosensor with inhibitor. Biosensors based on both antibody and inhibitor were found to be suitable for quantitative determination of the UCH-L1 and exhibit good tolerance to the potential interferents. Both biosensors gave comparable results in the range of 0 to 0.20 ng/ml for plasma samples and 0.30 to 0.49 ng/ml for cerebrospinal fluid samples. To validate the new methods, comparative determination of UCH-L1 by the commercial enzyme-linked immunosorbent assay (ELISA) kit was performed. In general, in terms of UCH-L1 concentration, a good correlation between SPRI and ELISA was found. The developed biosensors can be used successfully for the determination of UCH-L1 in body fluids. 相似文献
6.
Further studies of monoolein lipase from rat adipose tissue 总被引:1,自引:0,他引:1
7.
Krystyna Bogucka Lech Wojtczak 《Biochemical and biophysical research communications》1976,71(1):161-167
The intermembrane compartment of rat liver mitochondria contains high molecular weight compounds, most likely proteins, complexing magnesium ions. Compound/s/ of about 150 000 daltons has a low affinity /Kd 0.37 mM/ and a high binding capacity of 300 ng atoms Mg/mg protein; compound/s/ of about 100 000 daltons is characterized by a very high affinity towards magnesium /Kd of the order of magnitude of 0.001 mM/ and a low capacity of about 20 ng atoms Mg/mg protein. 相似文献
8.
Sławomir Borek Szymon Kubala Sylwia Kubala Lech Ratajczak 《Acta Physiologiae Plantarum》2011,33(5):1953-1968
Storage lipid and protein breakdown in germinating seeds of yellow (Lupinus luteus L.), white (L. albus L.), and Andean lupine (L. mutabilis Sweet) and regulatory function of sucrose were investigated. Less oil bodies were detected in organs of yellow lupine seeds,
whereas the highest content of oil bodies was noticed in the Andean lupine seeds. Mature, air-dried yellow, white and Andean
lupine seeds do not contain starch. Starch grains appear the earliest in white lupine seeds during imbibition. Sucrose deficiency
in tissues enhances breakdown of storage lipid, protein and temporary starch in cotyledons. In sucrose starved embryo axes
of all investigated lupine species, an increased level of vacuolization was noted. Interconnections between catabolism of
storage protein and storage lipid in germinating lupine seeds were identified by applying 14C-acetate. To assess the importance of key processes in storage lipid breakdown NaF (inhibitor of glycolysis and gluconeogenesis),
KCN, NaN3 and SHAM (inhibitors of mitochondrial electron transport chain) and MSO (inhibitor of glutamine synthetase) were used. Radioactivity
coming from 14C-acetate was released as 14CO2 but mostly was incorporated into ethanol-soluble fraction of embryo axes and cotyledons. Respiratory inhibitors caused a
significant decrease in 14CO2 and ethanol fractions in all three lupine species studied. MSO stimulated release of 14CO2 and radioactivity of ethanol fractions in yellow lupine organs fed with sucrose, but in Andean lupine MSO enhanced the production
of 14CO2 and radioactivity of ethanol fractions both in organs fed and not fed with sucrose. Different strategies of storage compound
breakdown are proposed, depending on relative proportion in storage protein and lipid content in lupine seeds. 相似文献
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10.
Bączyk Justyna Gogiel Tomasz Wolańska Małgorzata Bruczko Marta Guszczyn Tomasz Popko Janusz Romanowicz Lech 《International journal of peptide research and therapeutics》2020,26(1):271-280
International Journal of Peptide Research and Therapeutics - The progressive damage of human articular cartilage is associated with loss of integrity of its extracellular matrix components. Their... 相似文献