全文获取类型
收费全文 | 48827篇 |
免费 | 4169篇 |
国内免费 | 3911篇 |
出版年
2024年 | 25篇 |
2023年 | 482篇 |
2022年 | 640篇 |
2021年 | 1972篇 |
2020年 | 1660篇 |
2019年 | 1996篇 |
2018年 | 1950篇 |
2017年 | 1481篇 |
2016年 | 2000篇 |
2015年 | 3095篇 |
2014年 | 3626篇 |
2013年 | 3911篇 |
2012年 | 4578篇 |
2011年 | 4132篇 |
2010年 | 2700篇 |
2009年 | 2347篇 |
2008年 | 2966篇 |
2007年 | 2563篇 |
2006年 | 2309篇 |
2005年 | 1938篇 |
2004年 | 1746篇 |
2003年 | 1568篇 |
2002年 | 1323篇 |
2001年 | 836篇 |
2000年 | 637篇 |
1999年 | 618篇 |
1998年 | 443篇 |
1997年 | 382篇 |
1996年 | 365篇 |
1995年 | 343篇 |
1994年 | 309篇 |
1993年 | 223篇 |
1992年 | 279篇 |
1991年 | 229篇 |
1990年 | 159篇 |
1989年 | 150篇 |
1988年 | 107篇 |
1987年 | 135篇 |
1986年 | 110篇 |
1985年 | 93篇 |
1984年 | 59篇 |
1983年 | 46篇 |
1982年 | 54篇 |
1981年 | 31篇 |
1980年 | 24篇 |
1979年 | 28篇 |
1977年 | 21篇 |
1975年 | 23篇 |
1974年 | 20篇 |
1972年 | 18篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
Single Cell Transcriptome Amplification with MALBAC 总被引:1,自引:0,他引:1
3.
4.
Splenic gene expression profiling in White Leghorn layer inoculated with the Salmonella enterica serovar Enteritidis 下载免费PDF全文
Salmonella enterica serovar Enteritidis (SE) is a foodborne pathogen that can threaten human health through contaminated poultry products. Live poultry, chicken eggs and meat are primary sources of human salmonellosis. To understand the genetic resistance of egg‐type chickens in response to SE inoculation, global gene expression in the spleen of 20‐week‐old White Leghorn was measured using the Agilent 4 × 44 K chicken microarray at 7 and 14 days following SE inoculation (dpi). Results showed that there were 1363 genes significantly differentially expressed between inoculated and non‐inoculated groups at 7 dpi (I7/N7), of which 682 were up‐regulated and 681 were down‐regulated genes. By contrast, 688 differentially expressed genes were observed at 14 dpi (I14/N14), of which 371 were up‐regulated genes and 317 were down‐regulated genes. There were 33 and 28 immune‐related genes significantly differentially expressed in the comparisons of I7/N7 and I14/N14 respectively. Functional annotation revealed that several Gene Ontology (GO) terms related to immunity were significantly enriched between the inoculated and non‐inoculated groups at 14 dpi but not at 7 dpi, despite a similar number of immune‐related genes identified between I7/N7 and I14/N14. The immune response to SE inoculation changes with different time points following SE inoculation. The complicated interaction between the immune system and metabolism contributes to the immune responses to SE inoculation of egg‐type chickens at 14 dpi at the onset of lay. GC, TNFSF8, CD86, CD274, BLB1 and BLB2 play important roles in response to SE inoculation. The results from this study will deepen the current understanding of the genetic response of the egg‐type chicken to SE inoculation at the onset of egg laying. 相似文献
5.
6.
7.
Abstract Clostridium perfringens alpha-toxin was produced in a protein-hyperproducing strain, Bacillus brevis 47, by cloning the gene into the constructed expression-secretion vector which has the multiple promoters and the signal peptide coding region of an outer cell wall protein gene. The amount of alpha-toxin produced by the B. brevis 47 transformant carrying the gene was approximately 10 times greater than that produced by a B. subtilis transformant carrying the toxin gene. Biological activities and the N-terminal amino acid sequence of the toxin secreted by the B. brevis 47 transformant were identical to those of wild-type alpha-toxin. 相似文献
8.
Three saponins from Oxytropis species. 总被引:2,自引:0,他引:2
Three flavonoids and three saponins have been isolated from Oxytropis species. Their structures were determined as isorhamnetin-3-O-beta-D-glucoside, rhamnetin-3-O-beta-D-galactoside, apigenin, 3-O-[alpha-L-rhamnopyranosyl (1----2)-beta-D-glucopyranosyl(1----4)-beta-D-glucuronopyranosyl]+ ++soyasapogenol B, 3-O-[beta-D-glucopyranosyl(1----2)-beta-D-glucuronopyranosyl] azukisapogenol and a new saponin 3-O-[beta-D-glucopyranosyl(1----2)-beta-D-glucopyranosyl]-25-O-alpha-L- rhamnopyranosyl-(20S,24S)-3 beta,16 beta, 20,24,25-pentahydroxy-9,19-cycloanostane. 相似文献
9.
W. D. Roof H. Q. Fang K. D. Young Jianling Sun & Ry Young 《Molecular microbiology》1997,25(6):1031-1046
slyD encodes a 196 amino acid polypeptide that is a member of the FKBP family of cis–trans peptidyl–prolyl isomerases (PPIases). slyD mutations affect plaque formation by the phage φX174 by blocking the action of the phage lysis protein E. Here we describe the selection of a set of spontaneous slyD mutations conferring resistance to the expression of gene E from a plasmid. These mutations occur disproportionately in residues of SlyD that, based on the structure of the prototype mammalian FKBP12, make ligand contacts with immunosuppressing drug molecules or are conserved in other FKBP proteins. A wide variation in the plating efficiency of φX174 on these E R strains is observed, relative to the parental, indicating that these alleles differ widely in residual SlyD activity. Moreover, it is found that slyD mutations cause significant growth rate defects in Escherichia coli B and C backgrounds. Finally, overexpression of slyD causes filamentation of the host. Thus, among the FKBP genes found in organisms across the evolutionary spectrum, slyD is unique in having three distinct drug-independent phenotypes. 相似文献
10.