全文获取类型
收费全文 | 15359篇 |
免费 | 1241篇 |
国内免费 | 28篇 |
出版年
2023年 | 37篇 |
2022年 | 33篇 |
2021年 | 228篇 |
2020年 | 182篇 |
2019年 | 254篇 |
2018年 | 344篇 |
2017年 | 318篇 |
2016年 | 545篇 |
2015年 | 808篇 |
2014年 | 986篇 |
2013年 | 1028篇 |
2012年 | 1312篇 |
2011年 | 1119篇 |
2010年 | 716篇 |
2009年 | 655篇 |
2008年 | 943篇 |
2007年 | 862篇 |
2006年 | 825篇 |
2005年 | 721篇 |
2004年 | 711篇 |
2003年 | 597篇 |
2002年 | 539篇 |
2001年 | 331篇 |
2000年 | 307篇 |
1999年 | 254篇 |
1998年 | 129篇 |
1997年 | 105篇 |
1996年 | 83篇 |
1995年 | 96篇 |
1994年 | 82篇 |
1993年 | 58篇 |
1992年 | 154篇 |
1991年 | 112篇 |
1990年 | 105篇 |
1989年 | 101篇 |
1988年 | 82篇 |
1987年 | 76篇 |
1986年 | 68篇 |
1985年 | 79篇 |
1984年 | 64篇 |
1983年 | 42篇 |
1982年 | 47篇 |
1981年 | 39篇 |
1980年 | 33篇 |
1979年 | 49篇 |
1978年 | 39篇 |
1976年 | 31篇 |
1975年 | 36篇 |
1974年 | 39篇 |
1973年 | 40篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
Jung Mi Lim Kyung S. Lee Hyun Ae Woo Dongmin Kang Sue Goo Rhee 《The Journal of cell biology》2015,210(1):935-945
Proteins associated with the centrosome play key roles in mitotic progression in mammalian cells. The activity of Cdk1-opposing phosphatases at the centrosome must be inhibited during early mitosis to prevent premature dephosphorylation of Cdh1—an activator of the ubiquitin ligase anaphase-promoting complex/cyclosome—and the consequent premature degradation of mitotic activators. In this paper, we show that reversible oxidative inactivation of centrosome-bound protein phosphatases such as Cdc14B by H2O2 is likely responsible for this inhibition. The intracellular concentration of H2O2 increases as the cell cycle progresses. Whereas the centrosome is shielded from H2O2 through its association with the H2O2-eliminating enzyme peroxiredoxin I (PrxI) during interphase, the centrosome-associated PrxI is selectively inactivated through phosphorylation by Cdk1 during early mitosis, thereby exposing the centrosome to H2O2 and facilitating inactivation of centrosome-bound phosphatases. Dephosphorylation of PrxI by okadaic acid–sensitive phosphatases during late mitosis again shields the centrosome from H2O2 and thereby allows the reactivation of Cdk1-opposing phosphatases at the organelle. 相似文献
4.
5.
Eun Jin Park Joon Young Song Min Ju Choi Ji Ho Jeon Jah-yeon Choi Tae Un Yang Kyung Wook Hong Ji Yun Noh Hee Jin Cheong Woo Joo Kim 《The Korean journal of parasitology》2014,52(4):425-428
A 45-year-old-male who had underlying ulcerative colitis and presented with fever and dry cough. Initially, the patient was considered to have invasive aspergillosis due to a positive galactomannan assay. He was treated with amphotericin B followed by voriconazole. Nevertheless, the patient deteriorated clinically and radiographically. The lung biopsy revealed eosinophilic pneumonia, and ELISA for Toxocara antigen was positive, leading to a diagnosis of pulmonary toxocariasis. After a 10-day treatment course with albendazole and adjunctive steroids, the patient recovered completely without any sequelae. Pulmonary toxocariasis may be considered in patients with subacute or chronic pneumonia unresponsive to antibiotic agents, particularly in cases with eosinophilia. 相似文献
6.
Marjan M. Tajrishi Jonghyun Shin Michal Hetman Ashok Kumar 《The Journal of biological chemistry》2014,289(29):19985-19999
7.
8.
A continuous production of fructooligosaccharides from sucrose was investigated by fructosyltransferase immobilized on a high porous resin, Diaion HPA 25. The optimum pH (5.5) and temperature (55°C) of the enzyme for activity was unaltered by immobilization, and the immobilized enzyme became less sensitive to the pH change. The optimal operation conditions of the immobilized enzyme column for maximizing the productivity were as follows: 600 g/L of sucrose feed concentration, flow rate of superficial space velocity 2.7 h?1. When the enzyme column was run at 50°C, about 8% loss of the initial activity of immobilized enzyme was observed after 30 days of continuous operation, during which high productivity of 1174 g/L·h was achieved. The kinds of products obtained using the immobilized enzyme were almost the same as those using soluble enzymes or free cells. 相似文献
9.
10.
Theoretical calculation of the sugar concentrations during enzymatic production of fructooligosaccharides 总被引:1,自引:0,他引:1
Summary In a batch production of fructooligosaccharides from sucrose, the concentrations of residual sucrose, glucose and fructooligosaccharides at a given reaction time(t) and initial sucrose concentration(S0) were theoretically calculated by the following correlation equations: Glucose(t) = 0.0653 S0 × ln(t); Fructooligosaccharides(t) = 0.1636 S0 × ln(t); Sucrose(t)=S0 - Glucose(t) + FOS(t). 相似文献