全文获取类型
收费全文 | 1671篇 |
免费 | 147篇 |
出版年
2021年 | 13篇 |
2020年 | 14篇 |
2019年 | 12篇 |
2018年 | 15篇 |
2017年 | 20篇 |
2016年 | 25篇 |
2015年 | 35篇 |
2014年 | 34篇 |
2013年 | 71篇 |
2012年 | 80篇 |
2011年 | 78篇 |
2010年 | 51篇 |
2009年 | 41篇 |
2008年 | 78篇 |
2007年 | 76篇 |
2006年 | 68篇 |
2005年 | 57篇 |
2004年 | 77篇 |
2003年 | 74篇 |
2002年 | 76篇 |
2001年 | 72篇 |
2000年 | 73篇 |
1999年 | 51篇 |
1998年 | 33篇 |
1997年 | 26篇 |
1996年 | 22篇 |
1995年 | 32篇 |
1994年 | 26篇 |
1993年 | 21篇 |
1992年 | 45篇 |
1991年 | 54篇 |
1990年 | 33篇 |
1989年 | 41篇 |
1988年 | 26篇 |
1987年 | 25篇 |
1986年 | 16篇 |
1985年 | 22篇 |
1984年 | 23篇 |
1983年 | 18篇 |
1982年 | 13篇 |
1981年 | 13篇 |
1979年 | 16篇 |
1978年 | 15篇 |
1977年 | 13篇 |
1976年 | 15篇 |
1975年 | 9篇 |
1973年 | 12篇 |
1972年 | 8篇 |
1971年 | 13篇 |
1969年 | 7篇 |
排序方式: 共有1818条查询结果,搜索用时 41 毫秒
1.
Kazuma Nakano Akino Shiroma Makiko Shimoji Hinako Tamotsu Noriko Ashimine Shun Ohki Misuzu Shinzato Maiko Minami Tetsuhiro Nakanishi Kuniko Teruya Kazuhito Satou Takashi Hirano 《Human cell》2017,30(3):149-161
PacBio RS II is the first commercialized third-generation DNA sequencer able to sequence a single molecule DNA in real-time without amplification. PacBio RS II’s sequencing technology is novel and unique, enabling the direct observation of DNA synthesis by DNA polymerase. PacBio RS II confers four major advantages compared to other sequencing technologies: long read lengths, high consensus accuracy, a low degree of bias, and simultaneous capability of epigenetic characterization. These advantages surmount the obstacle of sequencing genomic regions such as high/low G+C, tandem repeat, and interspersed repeat regions. Moreover, PacBio RS II is ideal for whole genome sequencing, targeted sequencing, complex population analysis, RNA sequencing, and epigenetics characterization. With PacBio RS II, we have sequenced and analyzed the genomes of many species, from viruses to humans. Herein, we summarize and review some of our key genome sequencing projects, including full-length viral sequencing, complete bacterial genome and almost-complete plant genome assemblies, and long amplicon sequencing of a disease-associated gene region. We believe that PacBio RS II is not only an effective tool for use in the basic biological sciences but also in the medical/clinical setting. 相似文献
2.
3.
Surface antigens of Saccharomyces cerevisiae protoplast were visualized immunocytochemically with protein A-gold or ferritin conjugated antibodies. Ferritin particles were densely distributed at the surface of yeast protoplast after treatment with rabbit anti-protoplast serum and ferritin conjugated antibody against rabbit IgM. Control experiments demonstrated that the binding of these ferritin markers was specific for surface antigens. The composition of these surface antigens, however, remain to be investigated. 相似文献
4.
K Hirano M Fukuta T Adachi K Hayashi M Sugiura Y Mori K Toyoshi 《Biochemical and biophysical research communications》1985,129(1):89-94
The syntheses of copper, zinc-superoxide dismutase (Cu,Zn-SOD) and manganese-superoxide dismutase (Mn-SOD) in vitro were studied. Both Cu,Zn-SOD and Mn-SOD were preferentially synthesized by free polysomes. Mn-SOD was synthesized as a large precursor (26,000 daltons), which was processed to the mature size (22,500 daltons) by in vitro incubation with a rat liver mitochondrial fraction. On the other hand, Cu,Zn-SOD was synthesized as the mature size product. It was shown that Cu,Zn-SOD and Mn-SOD synthesized in vitro represented 0.018% and 0.016% of the total translation products of free polysomes, respectively. 相似文献
5.
6.
Large numbers of growth cones were present in 6-day-old primary cultures of cerebral hemispheres from fetal rats. The average size of the growth cones was 24 by 28 microns. Many of these growth cones had both veil-like lamellipodia and filopodia. A few cones remained in 21-day-old cultures. These also had lamellipodia and filopodia. Ganglioside GM1 was present in both 6-day-old and 21-day-old cultured growth cones. 相似文献
7.
A comparative study was made of the effects of 15 synthetic and naturally occurring flavonoids on the hydrolytic activity of Na+, K+-adenosine triphosphatase (ATPase). Twelve of the flavonoids examined were mono-hydroxy or mono-methoxy derivatives. All inhibited Na+, K+-ATPase from dog kidney cortex when present at concentrations from 40-1000 microM. Flavones possessing cyclohexyl instead of the phenyl group (i.e., 2-cyclohexyl-benzopyran-4-one derivatives), were the most potent with IC50 at 257-320 microM. Structure-activity relationships were observed among the following mono-substituted flavones as: i) 2-cyclohexyl-benzopyran-4-one much greater than 2-phenyl-benzopyran-4-one; ii) 2-cyclohexyl-7-hydroxybenzopyran-4-one greater than 2-cyclohexyl-6-hydroxybenzopyran-4-one greater than 2-cyclohexyl-5-hydroxybenzopyran-4-one. Some flavonoids showing potent inhibitory activity were also examined for ouabain-displacement activity on human erythrocytes. Hardly any of the flavonoids were able to block [3H]ouabain binding to erythrocytes. These results suggest that the mechanism by which flavonoid block Na+, K+-ATPase is not related to the cardiac glycoside-specific binding site(s) of this enzyme. 相似文献
8.
9.