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1.
Purification and characterization of a membrane-bound protein kinase from spinach thylakoids 总被引:3,自引:0,他引:3
A protein kinase was isolated from spinach thylakoid membranes by solubilization with octyl glucoside and cholate. The enzyme was purified to apparent homogeneity by ammonium sulfate precipitation, gel filtration, and sucrose density centrifugation, followed by affinity chromatography on either Affi-Gel blue (yielding denatured enzyme) or on histone cross-linked to Sepharose (yielding active enzyme). Electrophoresis on denaturing polyacrylamide gels, followed by staining with silver, revealed the kinase as a single band corresponding to an apparent molecular mass of 64 kDa. The active enzyme underwent autophosphorylation and could be detected by autoradiography following incubation with [gamma-32P]ATP and Mg2+ ion. The specific phosphotransferase activity of purified kinase was approximately 30 nmol of phosphate min-1 (mg protein)-1 with lysine-rich histone (III-S or V-S) as substrate; casein was phosphorylated at approximately 30% of this rate. The physiological substrate for the kinase is presumed to be light-harvesting chlorophyll a/b protein complex. In solubilized form, this was phosphorylated at approximately 10% of the rate observed with histone III-S as substrate, or 10-100 times slower than the estimated rate of phosphorylation of the light-harvesting complex in situ. Possible reasons for this shortfall are considered. The kinase is proposed as the principal effector of thylakoid protein phosphorylation and associated State transition phenomena. 相似文献
2.
Charles R.K. Hind Peter M. Collins Mark B. Pepys 《Biochimica et Biophysica Acta (BBA)/General Subjects》1984,802(1):148-150
Serum amyloid P component is a normal plasma glycoprotein which is the precursor of amyloid P component, a minor but universal constituent of amyloid deposits. When isolated human P component is exposed to free ionised Ca2+ it aggregates and precipitates. This phenomenon is completely inhibited by the presence of 10?4?10?2 M methyl 4,6-O-(1-car?yethylidene)-β-D-galactopyranoside, a recently synthesised specific ligand for amyloid P component. This observation suggests that the autoaggregation of human amyloid P component involves the Ca2+ dependent specific ligand binding property of P component, but does not distinguish between receptor-site-mediated and allosteric mechanisms. 相似文献
3.
Nicholas Hind 《Curtis's Botanical Magazine》2010,27(1):36-50
Adlumia fungosa (Aiton) Greene ex Britton, Sterns & Poggenb., (Papaveraceae: Fumarioideae: Corydaleae) is described and illustrated, and its cultivation and propagation discussed. The position of Adlumia within the family and tribe is discussed and a key to its separation from the only other climbing genus, Dactylicapnos, is provided. The true insertion point of the inflorescences is commented on. One hypothesis is put forward for the outgrowths on the underside of the upper rachis sections, petiolules, and midribs of the pinnules in assisting the cirrhose portions of the leaves to grip their support. The derivation of the generic name is commented on, and the correct etymology noted. A synopsis of Adlumia is provided. 相似文献
4.
5.
Nicholas Hind 《Curtis's Botanical Magazine》2019,36(3):202-225
Doronicum caucasicum M. Bieb. (Compositae: Senecioneae) is described and illustrated for the second time in the history of Curtis's Botanical Magazine. Critical examination and reading of the works in which the protologues were published has led to the conclusion that D. caucasicum was published earlier than D. orientale Hoffm., but it is highly likely that the two names were based on the same Adams collection that Adams had annotated as Doronicum orientale. The possibility of Doronicum L. being the sole genus in the recently proposed tribe Doroniceae Panero is discussed. The lack of any present infrageneric classification is commented upon, but this is probably due to insufficient characters in the multivariate analyses used, and where the limits are drawn in molecular analyses. Cultivation and propagation guidelines for the species are provided and currently availability of cultivars is outlined, as are likely pests and diseases. 相似文献
6.
Anthemis marschalliana Willd. (Compositae: Anthemideae: Anthemidinae) is described and illustrated, a full synonymy provided and its present taxonomic position discussed, especially the choice of this as the current name, and not Archanthemis marschalliana (Willd.) Lo Presti & Oberpr. The type collections of the accepted name and one synonym are compared as both honour the same person, Marschall von Bieberstein. Cultivation requirements are also outlined, with some commentary on the pests that are sometimes an issue. Cultivation and propagation requirements are outlined, and the availability of the species noted. 相似文献
7.
Abounouh Karima Kayesh Mohammad Enamul Hoque Altawalah Haya Kitab Bouchra Murakami Shuko Ogawa Shintaro Tanaka Yasuhito Dehbi Hind Pineau Pascal Kohara Michinori Benjelloun Soumaya Tsukiyama-Kohara Kyoko Ezzikouri Sayeh 《Molecular biology reports》2022,49(1):403-412
Molecular Biology Reports - Hepatitis B Virus (HBV) is the most common cause of chronic liver disease worldwide. The mechanisms that regulate HBV viral replication remain poorly defined. Here, we... 相似文献
8.
Deepak P. Edward Hind Alkatan Qundeel Rafiq Charles Eberhart Saleh Al Mesfer Nicola Ghazi Leen Al Safieh Altaf A. Kondkar Khaled K. Abu Amero 《PloS one》2015,10(3)
Purpose
To study the differential expression of microRNA (miRNA) profiles between intraocular medulloepithelioma (ME) and normal control tissue (CT).Material and Methods
Total RNA was extracted from formalin fixed paraffin embedded (FFPE) intraocular ME (n=7) and from age matched ciliary body controls (n=8). The clinical history and phenotype was recorded. MiRNA profiles were determined using the Affymetrix GeneChip miRNA Arrays analyzed using expression console 1.3 software. Validation of significantly dysregulated miRNA was confimed by quantitaive real-time PCR. The web-based DNA Intelligent Analysis (DIANA)-miRPath v2.0 was used to perform enrichment analysis of differentially expressed (DE) miRNA gene targets in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway.Results
The pathologic evaluation revealed one benign (benign non-teratoid, n=1) and six malignant tumors (malignant teratoid, n=2; malignant non-teratoid, n = 4). A total of 88 miRNAs were upregulated and 43 miRNAs were downregulated significantly (P<0.05) in the tumor specimens. Many of these significantly dysregulated miRNAs were known to play various roles in carcinogenesis and tumor behavior. RT-PCR validated three significantly upregulated miRNAs and three significantly downregulated miRNAs namely miR-217, miR-216a, miR-216b, miR-146a, miR-509-3p and miR-211. Many DE miRNAs that were significant in ME tumors showed dysregulation in retinoblastoma, glioblastoma, and precursor, normal and reactive human cartilage. Enriched pathway analysis suggested a significant association of upregulated miRNAs with 15 pathways involved in prion disease and several types of cancer. The pathways involving significantly downregulated miRNAs included the toll-like receptor (TLR) (p<4.36E-16) and Nuclear Factor kappa B (NF-κB) signaling pathways (p<9.00E-06).Conclusions
We report significantly dysregulated miRNAs in intraocular ME tumors, which exhibited abnormal profiles in other cancers as well such as retinoblastoma and glioblastoma. Pathway analysis of all dysregulated miRNAs shared commonalities with other cancer pathways. 相似文献9.
10.
Alastair G. Proudfoot Danny F. McAuley Mark J. D. Griffiths Matthew Hind 《Disease models & mechanisms》2011,4(2):145-153
Acute lung injury (ALI) is a syndrome that is characterised by acute inflammation and tissue injury that affects normal gas exchange in the lungs. Hallmarks of ALI include dysfunction of the alveolar-capillary membrane resulting in increased vascular permeability, an influx of inflammatory cells into the lung and a local pro-coagulant state. Patients with ALI present with severe hypoxaemia and radiological evidence of bilateral pulmonary oedema. The syndrome has a mortality rate of approximately 35% and usually requires invasive mechanical ventilation. ALI can follow direct pulmonary insults, such as pneumonia, or occur indirectly as a result of blood-borne insults, commonly severe bacterial sepsis. Although animal models of ALI have been developed, none of them fully recapitulate the human disease. The differences between the human syndrome and the phenotype observed in animal models might, in part, explain why interventions that are successful in models have failed to translate into novel therapies. Improved animal models and the development of human in vivo and ex vivo models are therefore required. In this article, we consider the clinical features of ALI, discuss the limitations of current animal models and highlight how emerging human models of ALI might help to answer outstanding questions about this syndrome. 相似文献