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Peng Wang Ronghua Luo Min Zhang Yaqing Wang Tianzhang Song Tingting Tao Zhongyu Li Lin Jin Hongyi Zheng Wenwen Chen Mengqian Zhao Yongtang Zheng Jianhua Qin 《Cell death & disease》2020,11(12)
COVID-19, caused by SARS-CoV-2, is an acute and rapidly developing pandemic, which leads to a global health crisis. SARS-CoV-2 primarily attacks human alveoli and causes severe lung infection and damage. To better understand the molecular basis of this disease, we sought to characterize the responses of alveolar epithelium and its adjacent microvascular endothelium to viral infection under a co-culture system. SARS-CoV-2 infection caused massive virus replication and dramatic organelles remodeling in alveolar epithelial cells, alone. While, viral infection affected endothelial cells in an indirect manner, which was mediated by infected alveolar epithelium. Proteomics analysis and TEM examinations showed viral infection caused global proteomic modulations and marked ultrastructural changes in both epithelial cells and endothelial cells under the co-culture system. In particular, viral infection elicited global protein changes and structural reorganizations across many sub-cellular compartments in epithelial cells. Among the affected organelles, mitochondrion seems to be a primary target organelle. Besides, according to EM and proteomic results, we identified Daurisoline, a potent autophagy inhibitor, could inhibit virus replication effectively in host cells. Collectively, our study revealed an unrecognized cross-talk between epithelium and endothelium, which contributed to alveolar–capillary injury during SARS-CoV-2 infection. These new findings will expand our understanding of COVID-19 and may also be helpful for targeted drug development.Subject terms: Mechanisms of disease, Viral infection 相似文献
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Haiyan Miao Jingjing Lu Yibing Guo Hongquan Qiu Yu Zhang Xihao Yao Xiaohong Li Yuhua Lu 《Journal of cellular and molecular medicine》2021,25(7):3654-3664
Pancreatic ductal adenocarcinoma (PDAC) is an invasive and aggressive cancer that remains a major threat to human health across the globe. Despite advances in cancer treatments and diagnosis, the prognosis of PDAC patients remains poor. New and more effective PDAC therapies are therefore urgently required. In this study, we identified a novel host factor, namely the LncRNA TP73-AS1, as overexpressed in PDAC tissues compared to adjacent healthy tissue samples. The overexpression of TP-73-AS1 was found to correlate with both PDAC stage and lymph node metastasis. To reveal its role in PDCA, we targeted TP73-AS1 using LnRNA inhibitors in a range of pancreatic cancer (PC) cell lines. We found that the inhibition of TP73-AS1 led to a loss of MMP14 expression in PC cells and significantly inhibited their migratory and invasive capacity. No effects of TP73-AS1 on cell survival or proliferation were observed. Mechanistically, we found that TP73-AS1 suppressed the expression of the known oncogenic miR-200a. Taken together, these data highlight the prognostic potential of TP73-AS1 for PC patients and highlight it as a potential anti-PDAC therapeutic target. 相似文献
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生物入侵是继栖息地破坏之后,全球生物多样性丧失的第二大驱动因素。近年来,原产于南美洲地区的斑纹小贻贝(Mytella strigata)在印度-西太平洋海区被陆续报道,而我国台湾、广东、海南、福建、广西等省份同样发现斑纹小贻贝,且其已经建立可自我维持的种群。但是,作为一种新型入侵生物,斑纹小贻贝尚未引起国内海洋管理部门和科研人员足够重视,亟待查明其在我国沿海的分布现状、扩散趋势和生态影响等,为斑纹小贻贝的检测、监测、防控和管理提供科学依据。综述了斑纹小贻贝的基础生物学特征和全球生物入侵现状,发现国内的斑纹小贻贝源于南美洲加勒比海地区,于2014年左右通过船舶压舱水或船体生物污损的形式侵入我国南方沿海并迅速扩散。此外,斑纹小贻贝在我国的生物入侵处于"引进-传播"阶段,即将大规模扩繁,因此亟需开展应急清除行动。 相似文献
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Lin Xiang Lin Shuang Liu Yuanlan Zhao Haiyan Wang Li Hasi Wuliji 《Plasmonics (Norwell, Mass.)》2018,13(5):1749-1758
Plasmonics - Large-scale ordered two-dimensional (2D) superlattices at oil/water interface were fabricated using single-crystal Au nanospheres (NSs) with different diameters as building blocks. A... 相似文献
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