全文获取类型
收费全文 | 745786篇 |
免费 | 77750篇 |
国内免费 | 481篇 |
出版年
2018年 | 6681篇 |
2016年 | 8934篇 |
2015年 | 12095篇 |
2014年 | 14168篇 |
2013年 | 20498篇 |
2012年 | 23200篇 |
2011年 | 23493篇 |
2010年 | 15702篇 |
2009年 | 14077篇 |
2008年 | 20841篇 |
2007年 | 21875篇 |
2006年 | 20487篇 |
2005年 | 19651篇 |
2004年 | 19320篇 |
2003年 | 18701篇 |
2002年 | 18408篇 |
2001年 | 33408篇 |
2000年 | 33718篇 |
1999年 | 26349篇 |
1998年 | 8725篇 |
1997年 | 9134篇 |
1996年 | 8656篇 |
1995年 | 8394篇 |
1994年 | 8255篇 |
1993年 | 8086篇 |
1992年 | 22285篇 |
1991年 | 21812篇 |
1990年 | 21381篇 |
1989年 | 20771篇 |
1988年 | 19465篇 |
1987年 | 18246篇 |
1986年 | 16990篇 |
1985年 | 17253篇 |
1984年 | 14172篇 |
1983年 | 12275篇 |
1982年 | 9380篇 |
1981年 | 8410篇 |
1980年 | 7781篇 |
1979年 | 13587篇 |
1978年 | 10482篇 |
1977年 | 9654篇 |
1976年 | 9021篇 |
1975年 | 10076篇 |
1974年 | 10796篇 |
1973年 | 10598篇 |
1972年 | 9805篇 |
1971年 | 8758篇 |
1970年 | 7703篇 |
1969年 | 7540篇 |
1968年 | 6966篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Cortney C. Winkle Leslie M. McClain Juli G. Valtschanoff Charles S. Park Christopher Maglione Stephanie L. Gupton 《The Journal of cell biology》2014,205(2):217-232
Developmental axon branching dramatically increases synaptic capacity and neuronal surface area. Netrin-1 promotes branching and synaptogenesis, but the mechanism by which Netrin-1 stimulates plasma membrane expansion is unknown. We demonstrate that SNARE-mediated exocytosis is a prerequisite for axon branching and identify the E3 ubiquitin ligase TRIM9 as a critical catalytic link between Netrin-1 and exocytic SNARE machinery in murine cortical neurons. TRIM9 ligase activity promotes SNARE-mediated vesicle fusion and axon branching in a Netrin-dependent manner. We identified a direct interaction between TRIM9 and the Netrin-1 receptor DCC as well as a Netrin-1–sensitive interaction between TRIM9 and the SNARE component SNAP25. The interaction with SNAP25 negatively regulates SNARE-mediated exocytosis and axon branching in the absence of Netrin-1. Deletion of TRIM9 elevated exocytosis in vitro and increased axon branching in vitro and in vivo. Our data provide a novel model for the spatial regulation of axon branching by Netrin-1, in which localized plasma membrane expansion occurs via TRIM9-dependent regulation of SNARE-mediated vesicle fusion. 相似文献
2.
3.
Theofilos Papadopoulos Rudolf Schemm Helmut Grubmüller Nils Brose 《The Journal of biological chemistry》2015,290(13):8256-8270
Signaling at nerve cell synapses is a key determinant of proper brain function, and synaptic defects—or synaptopathies—are at the basis of many neurological and psychiatric disorders. In key areas of the mammalian brain, such as the hippocampus or the basolateral amygdala, the clustering of the scaffolding protein Gephyrin and of γ-aminobutyric acid type A receptors at inhibitory neuronal synapses is critically dependent upon the brain-specific guanine nucleotide exchange factor Collybistin (Cb). Accordingly, it was discovered recently that an R290H missense mutation in the diffuse B-cell lymphoma homology domain of Cb, which carries the guanine nucleotide exchange factor activity, leads to epilepsy and intellectual disability in human patients. In the present study, we determined the mechanism by which the CbR290H mutation perturbs inhibitory synapse formation and causes brain dysfunction. Based on a combination of biochemical, cell biological, and molecular dynamics simulation approaches, we demonstrate that the R290H mutation alters the strength of intramolecular interactions between the diffuse B-cell lymphoma homology domain and the pleckstrin homology domain of Cb. This defect reduces the phosphatidylinositol 3-phosphate binding affinity of Cb, which limits its normal synaptogenic activity. Our data indicate that impairment of the membrane lipid binding activity of Cb and a consequent defect in inhibitory synapse maturation represent a likely molecular pathomechanism of epilepsy and mental retardation in humans. 相似文献
4.
Multistep Photoluminescence Decay Reveals Dissociation of Geminate Charge Pairs in Organolead Trihalide Perovskites 下载免费PDF全文
Ramūnas Augulis Marius Franckevičius Vytautas Abramavičius Darius Abramavičius Shaik Mohammed Zakeeruddin Michael Grätzel Vidmantas Gulbinas 《Liver Transplantation》2017,7(17)
Charge carrier dynamics in organolead iodide perovskites is analyzed by employing time‐resolved photoluminescence spectroscopy with several ps time resolution. The measurements performed by varying photoexcitation intensity over five orders of magnitude enable separation of photoluminescence components related to geminate and nongeminate charge carrier recombination and to address the dynamics of an isolated geminate electron–hole pair. Geminate recombination dominates at low excitation fluence and determines the initial photoluminescence decay. This decay component is remarkably independent of the material structure and experimental conditions. It is demonstrated that dependences of the geminate and nongeminate radiative recombination components on excitation intensity, repetition rate, and temperature, are hardly compatible with carrier trapping and exciton dissociation models. On the basis of semiclassical and quantum mechanical numerical calculation results, it is argued that the fast photoluminescence decay originates from gradual spatial separation of photogenerated weakly bound geminate charge pairs. 相似文献
5.
More than 50 hereditary lysosomal storage disorders (LSDs) are currently described. Most of these disorders are due to a deficiency of certain hydrolases/glycosidases and subsequent accumulation of nonhydrolyzable carbohydrate-containing compounds in lysosomes. Such accumulation causing hypertrophy of the lysosomal compartment is a characteristic feature of affected cells in LSDs. The investigation of biochemical and cellular parameters is of particular interest for understanding “life” of lysosomes in the normal state and in LSDs. This review highlights the wide spectrum of biochemical and morphological changes during developing LSDs that are extremely critical for many metabolic processes inside the various cells and tissues of affected persons. The data presented will help establish new complex strategies for metabolic correction of LSDs. 相似文献
6.
7.
8.
9.