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1.
2.
Ami Klein Sharon Ramcharitar Nevena Christeff Erik Nisbett-Brown Emmanuel Nunez Aaron Malkin 《In vitro cellular & developmental biology. Animal》1991,27(4):307-311
Summary These authors attempted to test the effect of anticoagulants on lymphocytes viability by reproducing the procedure used for
lymphocyte isolation for various immunologic tests in which blood specimens are allowed to stay at room temperature for 2
h before lymphocytes are isolated. Blood was obtained with three different anticoagulants i.e. heparin, citrate, and CPDA
(citrate, phosphate, dextrose, and adenine). Plasma was lyophilized and extracted with ethanol. Dried ethanol extracts were
suspended in medium (RPMI 1640+10% fetal bovine serum) and incubated with a lymphocyte cell line (MOLT-4). After 24 h of incubation
the viability of cells was examined. The following death rates of the cells were observed: heparin −63±4.6% (mean±SEM), citrate
−27±6.7%, and CPDA 6.2±0.6% (P<0.0005). A significant correlation was found between these results and changes in the concentrations of free fatty acids
in the extracts. These results emphasize the importance of choosing the right anticoagulant when the viability of lymphocytes
is obligatory. 相似文献
3.
Detection of interspecific competition between insects is often sensitive to scaling. We give an example of scale-dependent interference between the weevil Curculio elephas and the moth Cydia splendana, which both have larvae that develop in the fruits of chestnut Castanea sativa. Measures at three scales were considered: chestnut, husk (with one to three fertile fruits) and tree. Data come from observations in the field over 14 years, complemented by experiments done directly in trees. Data on individual chestnut fruits revealed a marked statistical interference between the two insects. Experiments demonstrated that presence of a moth larva in a fruit usually inhibits weevil egg-laying. Conversely, weevil presence does not strongly modify moth larval behavior. Cases of double infestation often correspond to fruits first attacked by the weevil. With measures on husks, interference between the two insects was observed only in some trees; its intensity was always weaker than in the chestnuts themselves. At the scale of entire trees, rates of infestation by each insect are not correlated. Interference in chestnut fruits is interpreted by assuming that the weevil female either is sensitive to a repellent molecule originating from a moth larva or its frass, or can detect moth larval sounds. Mechanisms governing infestation rates from data per tree are discussed in relation to those found at fruit scale and to plant-insect interactions. The need to estimate available resources both from quantitative and qualitative points of view is emphasized. 相似文献
4.
Amel Adel Emmanuel Abatih Niko Speybroeck Abdelkrim Soukehal Rachid Bouguedour Karim Boughalem Abdelmalek Bouhbal Mouloud Djerbal Claude Saegerman Dirk Berkvens 《PloS one》2015,10(3)
A large-scale study on canine Leishmania infection (CanL) was conducted in six localities along a west-east transect in the Algerian littoral zone (Tlemcen, Mostaganem, Tipaza, Boumerdes, Bejaia, Jijel) and covering two sampling periods. In total 2,184 dogs were tested with an indirect fluorescent antibody test (IFAT) and a direct agglutination test (DAT). Combined multiple-testing and several statistical methods were compared to estimate the CanL true prevalence and tests characteristics (sensitivity and specificity). The Bayesian full model showed the best fit and yielded prevalence estimates between 11% (Mostaganem, first period) and 38% (Bejaia, second period). Sensitivity of IFAT varied (in function of locality) between 86% and 88% while its specificity varied between 65% and 87%. DAT was less sensitive than IFAT but showed a higher specificity (between 80% and 95% in function of locality or/and season). A general increasing trend of the CanL prevalence was noted from west to east. A concordance between the present results and the incidence of human cases of visceral leishmaniasis was observed, where also a maximum was recorded for Bejaia. The results of the present study highlight the dangers when using IFAT as a gold standard. 相似文献
5.
Rudo F. Mapanga Uthra Rajamani Nonkululeko Dlamini Makhosazane Zungu-Edmondson Roisin Kelly-Laubscher Mohammed Shafiullah Athiq Wahab Mohamed Y. Hasan Mohamed A. Fahim Philippe Rondeau Emmanuel Bourdon M. Faadiel Essop 《PloS one》2012,7(10)
Diabetes constitutes a major health challenge. Since cardiovascular complications are common in diabetic patients this will further increase the overall burden of disease. Furthermore, stress-induced hyperglycemia in non-diabetic patients with acute myocardial infarction is associated with higher in-hospital mortality. Previous studies implicate oxidative stress, excessive flux through the hexosamine biosynthetic pathway (HBP) and a dysfunctional ubiquitin-proteasome system (UPS) as potential mediators of this process. Since oleanolic acid (OA; a clove extract) possesses antioxidant properties, we hypothesized that it attenuates acute and chronic hyperglycemia-mediated pathophysiologic molecular events (oxidative stress, apoptosis, HBP, UPS) and thereby improves contractile function in response to ischemia-reperfusion. We employed several experimental systems: 1) H9c2 cardiac myoblasts were exposed to 33 mM glucose for 48 hr vs. controls (5 mM glucose); and subsequently treated with two OA doses (20 and 50 µM) for 6 and 24 hr, respectively; 2) Isolated rat hearts were perfused ex vivo with Krebs-Henseleit buffer containing 33 mM glucose vs. controls (11 mM glucose) for 60 min, followed by 20 min global ischemia and 60 min reperfusion ± OA treatment; 3) In vivo coronary ligations were performed on streptozotocin treated rats ± OA administration during reperfusion; and 4) Effects of long-term OA treatment (2 weeks) on heart function was assessed in streptozotocin-treated rats. Our data demonstrate that OA treatment blunted high glucose-induced oxidative stress and apoptosis in heart cells. OA therapy also resulted in cardioprotection, i.e. for ex vivo and in vivo rat hearts exposed to ischemia-reperfusion under hyperglycemic conditions. In parallel, we found decreased oxidative stress, apoptosis, HBP flux and proteasomal activity following ischemia-reperfusion. Long-term OA treatment also improved heart function in streptozotocin-diabetic rats. These findings are promising since it may eventually result in novel therapeutic interventions to treat acute hyperglycemia (in non-diabetic patients) and diabetic patients with associated cardiovascular complications. 相似文献
6.
This paper explains the biophysical principles which, according to us, govern the Proteus mirabilis swarm phenomenon. Then, this explanation is translated into a mathematical model, essentially based on partial differential
equations. This model is then implemented using numerical methods of the finite volume type in order to make simulations.
The simulations show most of the characteristics which are observed in situ and in particular the terrace generation.
相似文献
7.
Brand Eva Junne Stefan Anane Emmanuel Cruz-Bournazou Mariano Nicolas Neubauer Peter 《Bioprocess and biosystems engineering》2018,41(9):1305-1313
Bioprocess and Biosystems Engineering - Large-scale bioreactors are inhomogeneous systems, in which the fluid phase expresses concentration gradients. They depend on the mass transfer and fluid... 相似文献
8.
Margot Tertrais Claire Bigot Emmanuel Martin Renaud Poincloux Arnaud Labrousse Isabelle Maridonneau-Parini 《European journal of cell biology》2021,100(4):151161
Phagocytosis consists in ingestion and digestion of large particles, a process strictly dependent on actin re-organization. Using synchronized phagocytosis of IgG-coated latex beads (IgG-LB), zymosan or serum opsonized-zymosan, we report the formation of actin structures on both phagocytic cups and closed phagosomes in human macrophages. Their lifespan, size, protein composition and organization are similar to podosomes. Thus, we called these actin structures phagosome-associated podosomes (PAPs). Concomitantly to the formation of PAPs, a transient disruption of podosomes occurred at the ventral face of macrophages. Similarly to podosomes, which are targeted by vesicles containing proteases, the presence of PAPs correlated with the maturation of phagosomes into phagolysosomes. The ingestion of LB without IgG did not trigger PAPs formation, did not lead to podosome disruption and maturation to phagolysosomes, suggesting that these events are linked together. Although similar to podosomes, we found that PAPs differed by being resistant to the Arp2/3 inhibitor CK666. Thus, we describe a podosome subtype which forms on phagosomes where it probably serves several tasks of this multifunctional structure. 相似文献
9.
Alayna E. Loiselle Shane A. J. Lloyd Emmanuel M. Paul Gregory S. Lewis Henry J. Donahue 《PloS one》2013,8(11)
Connexin 43 (Cx43) is the most abundant gap junction protein in bone and is required for osteoblastic differentiation and bone homeostasis. During fracture healing, Cx43 is abundantly expressed in osteoblasts and osteocytes, while Cx43 deficiency impairs bone formation and healing. In the present study we selectively deleted Cx43 in the osteoblastic lineage from immature osteoblasts through osteocytes and tested the hypothesis that Cx43 deficiency results in delayed osteoblastic differentiation and impaired restoration of biomechanical properties due to attenuated β-catenin expression relative to wild type littermates. Here we show that Cx43 deficiency results in alterations in the mineralization and remodeling phases of healing. In Cx43 deficient fractures the mineralization phase is marked by delayed expression of osteogenic genes. Additionally, the decrease in the RankL/ Opg ratio, osteoclast number and osteoclast size suggest decreased osteoclast bone resorption and remodeling. These changes in healing result in functional deficits as shown by a decrease in ultimate torque at failure. Consistent with these impairments in healing, β-catenin expression is attenuated in Cx43 deficient fractures at 14 and 21 days, while Sclerostin (Sost) expression, a negative regulator of bone formation is increased in Cx43cKO fractures at 21 days, as is GSK-3β, a key component of the β-catenin proteasomal degradation complex. Furthermore, we show that alterations in healing in Cx43 deficient fractures can be rescued by inhibiting GSK-3β activity using Lithium Chloride (LiCl). Treatment of Cx43 deficient mice with LiCl restores both normal bone formation and mechanical properties relative to LiCl treated WT fractures. This study suggests that Cx43 is a potential therapeutic target to enhance fracture healing and identifies a previously unknown role for Cx43 in regulating β-catenin expression and thus bone formation during fracture repair. 相似文献
10.
A number of d-galactopyranosides bearing aromatic substituents have been prepared, and their binding to immunoglobulin J539 (Fab') has been studied. It appears that the main contribution of the 6-O-aromatic moiety to binding arises from the fact that it imparts an increased hydrophobicity to the ligand, causing a decrease in its hydration (solubility) that results in a greater free-energy of binding. In the d-galactosides having an aromatic aglycon, the phenyl group appears to partake in actual interactions with the protein. 相似文献