Quantitative estimation of islet tissue of pancreas in Spinifex hopping mouse (Notomys alexis)

Pancreases from three male and three female adult Spinifex hopping mice ( Notomys alexis ) were studied. No correlation was found between pancreas weight and body weight. Estimations of islet tissue mass and of individual cell types were made on paraffin sections of Bouin-fixed tissue taken from head, neck, body and tail regions of pancreas of each animal. Islet tissue mass was assessed using a linear scanning technique on sections stained with haematoxylin and eosin, and was compared with body weight. Specific cell types were assessed using a point-intercept method, on aldehyde-fuchsin-stained sections for beta (β) cells, and on immunoperoxidase labelled sections for alpha (α) cells (glucagon) and delta (δ) cells (somatostatin). Positive regional differences noted were a greater proportion of islet tissue in the tail region, and a lower proportion of α cells in the head region. Alpha cells were peripherally situated in the islets.
These results show some elements of agreement with a previously proposed hypothesis regarding the general patterns of arrangement of the mammalian endocrine pancreas.     

Water Balance in the Land Crab, Gecarcinus lateralis, During the Intermolt Cycle

Gecarcinus lateralis can take moisture from a clamp substratumin amounts adequate for the needs of the entire intermolt cycle.It can also rehydrate in this way, even after severe dehydration. This crab is able to survive for many months when free waterof a wide range of salinities (0.30; = parts per thousand)is made available in a shallow dish. The crab dies within sevenweeks when the salinity of this water is 35. During proecdysisthe pericardial sacs of eyestalkless crabs become most swollenwhen the salinity of the available water is 15 or 23, and survivalduring and after ecdysis is greatest with water of 15. A crab in proecdysis shows no increase in the rate at whichwater enters following dehydration. Yet large amounts of waterare retained, particularly at the intermediate salinities. Maximalswelling of the pericardial sacs just prior to ecdysis is essentiallyequivalent in crabs with eyestalks, in eyestalkless crabs, andin eyestalkless crabs that have received an implant of centralnervous tissue. Hence, we conclude that a hormone causing theretention of water exists, but not in the eyestalks, in thebrain, or in the thoracic ganglionic mass. At ecdysis eyestalkless crabs show large increases in the dimensionsof the carapace, while crabs with eyestalks and eyestalklesscrabs that have received an implant of certain central nervoustissues show much less increase and may even show a decrease.Thus, we conclude that a hormone causing a release of waterat ecdysis is produced in the central nervous system. The advantages to the crab of a dual hormonal control of itswater balance are discussed.     

Genus Pleistophora Gurley, 1893: An Assemblage of at Least Three Genera1

Until recently, pansporoblastic microsporidia that produce a variable and large number of sporoblasts from a sporont have been included in a single genus, namely Pleistophora Gurley, 1893. Ultrastructural studies have been used to determine whether the resemblance of these species is fundamental or superficial. The results indicated that the multisporous pansporoblastic forms belong to at least three genera and, thus, that Pleistophora is a “composite genus.” The term pansporoblast was originally used for stages in myxosporidian development. The term sporophorous vesicle adopted from Gurley is suggested for the spore-containing vesicle in the Microspora. Three species were studied: Pleistophora typicalis, the type-species; Pleistophora culicis, for which a new genus Vavraia has already been proposed; and Pleistophora simulii. P. typicalis and V. culicis have isolated nuclei throughout their development, and the sporophorous vesicle wall enveloping the sporoblasts is derived from amorphous secretions laid down during merogony external to the plasmalemma. Pleistophora and Vavraia are differentiated principally in terms of the structure of the sporophorous vesicle wall and mode of division of the sporogonial plasmodium. The nuclei of young sporonts of P. simulii are in diplokaryon arrangement and undergo meiosis to give haploid nuclei in the sporoblasts. The sporophorous vesicle wall is membranoid and is laid down external to the plasmalemma at the onset of sporogony. A new genus, Polydispyrenia n. g., is suggested for this species, the affinities of which are closer to the dimorphic species of microsporidia than to Pleistophora or Vavraia. The terms “merontogenetic sporophorous vesicle” and “sporontogenetic sporophorous vesicle” are used to distinguish between the two groups.     

The scales of salticid spiders

The form and location of the scales of salticid spiders, as revealed by light and scanning electron microscopy, provide useful diagnostic characters for the separation of species, the assignment of species to genera, and a further understanding of the relationships between salticid genera. Partly on the basis of distinctions provided by scale structure, the new genus Platycryptus , type-species Aranea undata De Geer, 1778, is defined. Metaphidippus vitis Cockerell, 1894, is placed in the genus Sassacus Peckham, 1895, and Paromaevia michelsoni (Barnes), 1955, is returned to the genus Maevia . The placement of Eris, Hentzia, Icius, Metaphidippus, Phidippus , and Sassacus in the subfamily Dendryphantinae is substantiated; Tutelina and Zygoballus are added to this group. On the basis of common scale structure, Evarcha, Habrocestum, Menememerus, Phlegra, Platycryptus , and Sitticus are tentatively assigned to the subfamily Habrocestinae. Scales of Anasntis, Corythalia, Cosmophasis, Hyllus, Marpissa, Metacyrba, Pellenes, Plexippus, Salticus, Sarinda, and Thiodina are also described.     

Morphometric Studies of Mitochondria in Tetrahymena pyriformis Exposed to Chloramphenicol or Ethidium Bromide*

Cultures of Tetrahymena pyriformis strain ST were exposed to 300 μg chloramphenicol/ml or 15 μg ethidium bromide/ml for 48 hr. Qualitative assessments of electron micrographs reveal that the abundance of mitochondrial cristae decreases greatly. By equating the spatial characteristics of the organism with those of a prolate spheroid, the distribution and abundance of mitochondria were quantified. Such characterizations reveal that the size of individual mitochondria decreases by 35–60% and that the number of mitochondria/cell increases ～8 fold. The observations are discussed in terms of coordinated mitochondrial and nuclear genetic activities.     

Climate‐growth analysis for a Mexican dry forest tree shows strong impact of sea surface temperatures and predicts future growth declines

Tropical forests will experience relatively large changes in temperature and rainfall towards the end of this century. Little is known about how tropical trees will respond to these changes. We used tree rings to establish climate‐growth relations of a pioneer tree, Mimosa acantholoba, occurring in tropical dry secondary forests in southern Mexico. The role of large‐scale climatic drivers in determining interannual growth variation was studied by correlating growth to sea surface temperature anomalies (SSTA) of the Atlantic and Pacific Oceans, including the El Niño‐Southern Oscillation (ENSO). Annual growth varied eightfold over 1970–2007, and was correlated with wet season rainfall (r=0.75). Temperature, cloud cover and solar variation did not affect growth, although these climate variables correlated with growth due to their relations with rainfall. Strong positive correlations between growth and SSTA occurred in the North tropical Atlantic during the first half of the year, and in the Pacific during the second half of the year. The Pacific influence corresponded closely to ENSO‐like influences with negative effects of high SSTA in the eastern Pacific Niño3.4 region on growth due to decreases in rainfall. During El Niño years growth was reduced by 37%. We estimated how growth would be affected by the predicted trend of decreasing rainfall in Central America towards the end of this century. Using rainfall predictions of two sets of climate models, we estimated that growth at the end of this century will be reduced by 12% under a medium (A1B) and 21% under a high (A2) emission scenario. These results suggest that climate change may have repercussions for the carbon sequestration capacity of tropical dry forests in the region.     

Natural enemies of natural enemies: the potential top‐down impact of predators on entomopathogenic nematode populations

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Short-term Effects of Ferulic Acid on Ion Uptake and Water Relations in Cucumber Seedlings

Ferulic acid (FA) is commonly found in soils and is consideredan allelochemical. Studies have suggested that FA and otherphenolic acids decrease plant growth in part by decreasing theabsorption of mineral nutrients and water. However, no studieshave examined these parameters in a single experimental systemto investigate how FA affected both ion uptake and plant-waterrelations in whole plants. Using intact cucumber (Cucumis sativusL. cv. Early Green Cluster) seedlings, we examined short-termeffects of FA on ion uptake kinetics, transport promoters andinhibitors, and water relations as indicated by a pressure-volumeanalysis. We found that after 3 h of treatment, 200 µMFA inhibited net ion uptake, particularly NO₃, and promotednet K⁺ efflux from seedling roots. The addition of fusicoccin,a K⁺ transport promoter, counteracted the inhibitory effectof FA on net K⁺ uptake. Concurrent treatment of seedlings withFA and tetraethylammonium, a channel-blocking salt, reducedaverage K⁺ efflux by 66%. Treatment of seedlings with FA alsodecreased leaf water potential (₁ and turgor pressure (P_T).However, decreased ₁ and P_T were not caused by changes in theosmotic properties of the symplast or stomatal conductance.A decrease in water absorption is a likely explanation for theloss of P_T observed. The results of our experiments indicatethat both ion uptake and plant-water relations can be adverselyaffected by FA. Key words: Cucumis sativus, ferulic acid, allelopathy, ion uptake, water relations     

Global Invasions of Marine and Estuarine Habitats by Non-Indigenous Species: Mechanisms, Extent, and Consequences

Non-indigenous species (NIS) are increasingly conspicuous inmarine and estuarine habitats throughout the world, as the number,variety, and effects of these species continue to accrue. Mostof these NIS invasions result from anthropogenic dispersal.Although the relative importance of different dispersal mechanismsvaries both spatially and temporally, the global movement ofballast water by ships appears to be the largest single vectorfor NIS transfer today, andmany recent invasions have resultedfrom this transfer. The rate of new invasions may have increasedin recent decades, perhaps due to changes in ballast water transport.Estuaries have been especially common sites of invasions, accumulatingfrom tens to hundreds of NIS per estuary that include most majortaxonomic and trophic groups. We now know of approximately 400NIS along the Pacific, Atlantic and Gulf coasts of the U.S.,and hundreds of marine and estuarine NIS are reported from otherregions of the world. Although available information about invasionsis limited to a few regions and underestimates the actual numberof NIS invasions, there are apparent differences in the frequencyof NIS among sites. Mechanisms responsible for observed patternsamong sites likely include variation in supply of NIS, and perhapsvariation in properties of recipient or donor communities,butthe role of these mechanisms has not been tested. Although ourpresent knowledge about the extent, patterns and mechanismsof marine invasions is still in its infancy, it is clear thatNIS are a significant force of change in marine and especiallyestuarine communities globally. Taxonomically diverse NIS arehaving significant effects on many, if not most, estuaries thatfundamentally alter population, community, and ecosystems processes.The impacts of most NIS remain unknown, and the predictabilityof their direct and indirect effects remains uncertain. Nonetheless,based upon the documented extent of NIS invasions and scopeof then effects, studies of marine communities that do not includeNIS are increasingly incomplete