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1.
David A. Dik Daniel R. Marous Jed F. Fisher 《Critical reviews in biochemistry and molecular biology》2017,52(5):503-542
The lytic transglycosylases (LTs) are bacterial enzymes that catalyze the non-hydrolytic cleavage of the peptidoglycan structures of the bacterial cell wall. They are not catalysts of glycan synthesis as might be surmised from their name. Notwithstanding the seemingly mundane reaction catalyzed by the LTs, their lytic reactions serve bacteria for a series of astonishingly diverse purposes. These purposes include cell-wall synthesis, remodeling, and degradation; for the detection of cell-wall-acting antibiotics; for the expression of the mechanism of cell-wall-acting antibiotics; for the insertion of secretion systems and flagellar assemblies into the cell wall; as a virulence mechanism during infection by certain Gram-negative bacteria; and in the sporulation and germination of Gram-positive spores. Significant advances in the mechanistic understanding of each of these processes have coincided with the successive discovery of new LTs structures. In this review, we provide a systematic perspective on what is known on the structure–function correlations for the LTs, while simultaneously identifying numerous opportunities for the future study of these enigmatic enzymes. 相似文献
2.
The ‘benefits of philopatry’ hypothesis states that helpers in cooperatively breeding species derive higher benefits from
remaining home, instead of dispersing and attempting to breed independently. We tested experimentally whether dispersal options
influence dispersal propensity in the cooperatively breeding Lake Tanganyika cichlids Neolamprologus pulcher and N. savoryi. Cooperative groups of these fishes breed in densely packed colonies, surrounded by unoccupied, but apparently suitable breeding
habitat. Breeding inside colonies and living in groups seems to benefit individuals, for example by early detection and deterrence
of predators. We show that despite a slight preference of both species for habitat with a higher stone cover, 40% of the preferred
habitat remained unoccupied. On average, the colonies contained a higher number of (1) predators of adults, juveniles and
eggs, (2) shelter competitors, and (3) other species including potential food competitors, compared to the outside colony
habitat. Apparently, habitat differences cannot explain why these cichlids breed in colonies. Accordingly, dispersal may not
be limited by a lack of suitable breeding shelters, but by the relatively higher risk of establishing an outside- compared
to a within-colony breeding territory. To test whether cichlids prefer within- to outside-colony breeding territories, we
provided breeding shelters inside the colony and at the colony edge and studied helper dispersal. As expected, significantly
more shelters were occupied within the colony compared to the edge. New breeding pairs with several helpers occupied these
shelters. We conclude that although breeding habitat is plentiful outside the colonies, helpers delay dispersal to obtain
a higher quality breeding position within the group or colony eventually, or they disperse in groups. Our results suggest
that (1) group augmentation and Allee effects are generally important for dispersal decisions in cooperatively breeding cichlids,
consistent with the ‘benefits of philopatry hypothesis’, and (2) habitat saturation cannot fully explain delayed dispersal
in these species. 相似文献
3.
Luc E. Coffeng Wilma A. Stolk Achim Hoerauf Dik Habbema Roel Bakker Adrian D. Hopkins Sake J. de Vlas 《PloS one》2014,9(12)
The African Programme for Onchocerciasis Control (APOC) is currently shifting its focus from morbidity control to elimination of infection. To enhance the likelihood of elimination and speed up its achievement, programs may consider to increase the frequency of ivermectin mass treatment from annual to 6-monthly or even higher. In a computer simulation study, we examined the potential impact of increasing the mass treatment frequency for different settings. With the ONCHOSIM model, we simulated 92,610 scenarios pertaining to different assumptions about transmission conditions, history of mass treatment, the future mass treatment strategy, and ivermectin efficacy. Simulation results were used to determine the minimum remaining program duration and number of treatment rounds required to achieve 99% probability of elimination. Doubling the frequency of treatment from yearly to 6-monthly or 3-monthly was predicted to reduce remaining program duration by about 40% or 60%, respectively. These reductions come at a cost of additional treatment rounds, especially in case of 3-monthly mass treatment. Also, aforementioned reductions are highly dependent on maintained coverage, and could be completely nullified if coverage of mass treatment were to fall in the future. In low coverage settings, increasing treatment coverage is almost just as effective as increasing treatment frequency. We conclude that 6-monthly mass treatment may only be worth the effort in situations where annual treatment is expected to take a long time to achieve elimination in spite of good treatment coverage, e.g. because of unfavorable transmission conditions or because mass treatment started recently. 相似文献
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Pesticide ecotoxicological effect factors and their uncertainties for freshwater ecosystems 总被引:1,自引:1,他引:0
Rosalie van Zelm Mark A. J. Huijbregts Leo Posthuma Arjen Wintersen Dik van de Meent 《The International Journal of Life Cycle Assessment》2009,14(1):43-51
Background, aim, and scope Characterization factors for ecotoxicity in the Life Cycle Impact Assessment (LCIA) are used to convert emissions into ecotoxicological
impacts. Deriving them involves a fate and an effect analysis step. The fate factor quantifies the change in environmental
concentration per unit of emission, while the effect factor quantifies the change in impact on the ecosystem per unit of environmental
concentration. This paper calculates freshwater ecotoxicological effect factors for 397 pesticides belonging to 11 pesticide-specific
toxic modes of action (TMoA), such as acetylcholinesterase inhibition and photosynthesis inhibition. Moreover, uncertainties
in the effect factors due to uncertain background concentrations and due to limited toxicity data are quantified.
Methods To calculate median ecotoxicological effect factors (EEFs), toxic pressure assessments were made, based on the species sensitivity
distribution—and the multisubstance potentially affected fraction—concept. The EEF quantifies an estimate of the fraction
of species that is probably affected due to a marginal change in concentration of a pesticide. EEFs were divided into a TMoA-specific
and a chemical-specific part, which were calculated on the basis of physicochemical properties, emissions, and toxicity data.
Propagation of parameter uncertainty in the EEFs and the TMoA- and chemical-specific parts was quantified by Monte Carlo simulation
and results were reported as 90% confidence intervals.
Results Median EEFs range from 2·10−3 to 7·106 l/g. Uncertainty in the TMoA-specific part is dominated by uncertainty in the TMoA-specific spread in species sensitivity
and by uncertainty in the effective toxicity of a TMoA. Uncertainty in the chemical-specific part of the EEFs depends on the
number of species for which toxicity data are available to calculate average toxicity (n
s) and ranges from a median uncertainty of 2.6 orders of magnitude for n
s = 2 to one order of magnitude for n
s ≥ 4. The TMoA-specific effect factor for systemic fungicides shows the largest uncertainty range. For seven TMoAs, uncertainty
ranges of the TMoA-specific effect factor are less than two orders of magnitude. For the other four TMoAs, the EEF uncertainty
range is between two and eight orders of magnitude. For the chemical-specific part of the EEFs, we found that variation in
uncertainty readily decreases for pesticides for which toxicity data are available for at least three species.
Discussion The same parameters that contributed most to uncertainty were found for pesticides as were found before for high-production-volume
chemicals. However, uncertainty in concentrations of pesticides was lower. TMoA-specific factors obtained with the applied
nonlinear method differ up to nine orders of magnitude from the factor of 0.5, which is used in the linear method. With the
applied method, a distinction in EEFs can be made among different TMoAs.
Conclusions Ecotoxicological effect factors are presented, including overviews of their uncertainty ranges and the main contributors to
uncertainty. The applied nonlinear method provides the possibility to quantify parameter uncertainty in the TMoA-specific
part of the ecotoxicological effect factor, which is helpful to get more insight in how uncertainty in ecotoxicological characterization
factors can be reduced.
Recommendations and perspectives The calculated uncertainty ranges can be included in life cycle assessment (LCA) case studies, which allows for better interpretation
of LCA results obtained with the EEFs. To put the uncertainty in effect factors into perspective within LCIA, more information
on the uncertainty in fate factors should be derived.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
8.
Genetic relatedness in groups is sex-specific and declines with age of helpers in a cooperatively breeding cichlid 总被引:3,自引:0,他引:3
Kin selection can explain the evolution of cooperative breeding and the distribution of relatives within a population may influence the benefits of cooperative behaviour. We provide genetic data on relatedness in the cooperatively breeding cichlid Neolamprologus pulcher. Helper to breeder relatedness decreased steeply with increasing helper age, particularly to the breeding males. Helper to helper relatedness was age‐assortative and also declined with age. These patterns of relatedness could be attributed to territory take‐overs by outsiders when breeders had disappeared (more in breeding males), between‐group dispersal of helpers and reproductive parasitism. In six of 31 groups females inherited the breeding position of their mother or sister. These matrilines were more likely to occur in large groups. We conclude that the relative fitness benefits of helping gained through kin selection vs. those gained through direct selection depend on helper age and sex. 相似文献
9.
Neolamprologus pulcher is a cooperatively breeding cichlid fish,in which helpers stay in their natal territory and help withbrood care, territory defense, and maintenance. In this studywe investigated helper effects by an experimental group sizereduction in the field. After this manipulation, focal helpersin reduced groups tended to feed less, and small helpers visitedthe breeding shelter significantly more often than same-sizedhelpers in control groups. No evidence was found that remaininghelpers compensated for the removed helpers by increasing territorydefense and maintenance behavior. Breeders, however, did showa lower defense rate, possibly caused by an increase in broodcare effort. Survival of fry was significantly lower in removalthan control groups, which provides the first experimental proofin a natural population of fish that brood care helpers do effectivelyhelp. The data suggest that in small, generally younger, helpers,kin selection may be an important evolutionary cause of cooperation.Large helpers, however, who are generally older and less relatedto the breeders than small helpers are suggested to pay to beallowed to stay in the territory by helping. All group membersbenefit from group augmentation. 相似文献
10.
In most species, some individuals delay reproduction or occupy inferior breeding positions. The queue hypothesis tries to explain both patterns by proposing that individuals strategically delay breeding (queue) to acquire better breeding or social positions. In 1995, Ens, Weissing, and Drent addressed evolutionarily stable queuing strategies in situations with habitat heterogeneity. However, their model did not consider the non-mutually exclusive individual quality hypothesis, which suggests that some individuals delay breeding or occupy inferior breeding positions because they are poor competitors. Here we extend their model with individual differences in competitive abilities, which are probably plentiful in nature. We show that including even the smallest competitive asymmetries will result in individuals using queuing strategies completely different from those in models that assume equal competitors. Subsequently, we investigate how well our models can explain settlement patterns in the wild, using a long-term study on oystercatchers. This long-lived shorebird exhibits strong variation in age of first reproduction and territory quality. We show that only models that include competitive asymmetries can explain why oystercatchers' settlement patterns depend on natal origin. We conclude that predictions from queuing models are very sensitive to assumptions about competitive asymmetries, while detecting such differences in the wild is often problematic. 相似文献