排序方式: 共有59条查询结果,搜索用时 328 毫秒
1.
Isolated nuclei of rat hepatocytes were incubated with 0.05% sodium heparinate for 2 to 10 min. Alterations in the nuclei were controlled biochemically, determining the amounts of DNA and histones, and by cytophotometric methods determining the amounts of total and nonhistone proteins and DNA. Under the selected experimental conditions 95% of histones are bound already after 5-min incubation with heparin; nonhistone proteins of cell nuclei remain unchanged. The blockade of histones is followed by DNA diffusion into the incubation medium. Experiments with nuclear staining with alcian blue proved the specificity of heparin binding with histones and showed that heparin-histone complex remains in the nuclei, and its histones lose their extractability with 0.25 n HCl. 相似文献
2.
3.
4.
Summary Isolated nuclei of rat hepatocytes were incubated with 0.05% sodium heparinate for 2 to 10 min. Alterations in the nuclei were controlled biochemically, determining the amounts of DNA and histones, and by cytophotometric methods determining the amounts of total and nonhistone proteins and DNA. Under the selected experimental conditions 95% of histones are bound already after 5-min incubation with heparin; nonhistone proteins of cell nuclei remain unchanged. The blockade of histones is followed by DNA diffusion into the incubation medium. Experiments with nuclear staining with alcian blue proved the specificity of heparin binding with histones and showed that heparin-histone complex remains in the nuclei, and its histones lose their extractability with 0.25 n HCl. 相似文献
5.
6.
Yu. E. Demidenko 《Paleontological Journal》2016,50(5):435-449
Morphology of the phosphatic sclerites of the species Mobergella radiolata that come from the Lower Cambrian sections is studied, and the stratigraphic distribution of the species is revised. The sclerite laminated microstructure of this species is described and is shown to be similar to that of some gastropods, bivalves, brachiopods, bryozoans, and corals. The species M. hexactina Skovsted, 2003 and M. sibirica Skovsted, 2003 are shown to be junior synonyms of M. radiolata based on sclerite morphology supported by the results of statistical analysis. 相似文献
7.
Lunina O. N. Savvichev A. S. Babenko V. V. Boldyreva D. I. Kuznetsov B. B. Kolganova T. V. Krasnova E. D. Kokryatskaya N. M. Veslopolova E. F. Voronov D. A. Demidenko N. A. Letarova M. A. Letarov A. V. Gorlenko V. M. 《Microbiology》2019,88(1):100-114
Microbiology - The community of anoxygenic phototrophic bacteria (APB) from the water column of the meromictic Lake Trekhtsvetnoe (Kandalaksha Bay, White Sea, Russia) was studied in March 2012 and... 相似文献
8.
9.
10.
Savvichev A. S. Kulakova A. A. Krasnova E. D. Voronov D. A. Kadnikov V. V. Beletskii A. V. Kozyaeva V. V. Rusanov I. I. Letarova M. A. Veslopolova E. F. Belenkova V. V. Demidenko N. A. Gorlenko V. M. 《Microbiology》2022,91(4):432-444
Microbiology - The composition of microbial communities and the rates of microbial processes in the water column of a meromictic trough in the Biofilter Bay, Kandalaksha Bay, White Sea were... 相似文献