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Variability within a pea core collection of LEAM and HSP22, two mitochondrial seed proteins involved in stress tolerance 下载免费PDF全文
MARIE‐HÉLÈNE AVELANGE‐MACHEREL NICOLE PAYET DAVID LALANNE MARTINE NEVEU DIMITRI TOLLETER JUDITH BURSTIN DAVID MACHEREL 《Plant, cell & environment》2015,38(7):1299-1311
LEAM, a late embryogenesis abundant protein, and HSP22, a small heat shock protein, were shown to accumulate in the mitochondria during pea (Pisum sativum L.) seed development, where they are expected to contribute to desiccation tolerance. Here, their expression was examined in seeds of 89 pea genotypes by Western blot analysis. All genotypes expressed LEAM and HSP22 in similar amounts. In contrast with HSP22, LEAM displayed different isoforms according to apparent molecular mass. Each of the 89 genotypes harboured a single LEAM isoform. Genomic and RT‐PCR analysis revealed four LEAM genes differing by a small variable indel in the coding region. These variations were consistent with the apparent molecular mass of each isoform. Indels, which occurred in repeated domains, did not alter the main properties of LEAM. Structural modelling indicated that the class A α‐helix structure, which allows interactions with the mitochondrial inner membrane in the dry state, was preserved in all isoforms, suggesting functionality is maintained. The overall results point out the essential character of LEAM and HSP22 in pea seeds. LEAM variability is discussed in terms of pea breeding history as well as LEA gene evolution mechanisms. 相似文献
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Studying a species under a range of conditions is essential for fully understanding its ecology and for predicting its response to human impacts on the environment. We investigated the spatial behaviour and the habitat characteristics of foraging areas of Eurasian Stone‐curlews breeding in an important but poorly investigated habitat, gravel riverbed, throughout the full 24‐h cycle. The data collected for 17 radiotagged birds nesting in the Taro River Regional Park (Parma, Italy) showed a clear split between diurnal and nocturnal spatial behaviour. Almost all diurnal fixes and about two‐thirds of nocturnal ones were located in the gravel riverbed, which not only provided suitable breeding territories but probably part of the food resources needed for reproduction. Nocturnal excursions from breeding sites to feeding areas (mostly farmland), sometimes of a few kilometres, indicated that these resources do not cover all of the birds’ needs. Night spotlight counts of foraging birds in the agricultural area indicated that Stone‐curlews preferred recently harvested crops (mainly forage and wheat) and piles of farmyard manure. These habitat preferences are closely linked to the predominant agricultural activity of the study area, which is characterized by a high density of dairy farms for the production of Parmigiano Reggiano cheese. It seems likely that the proximity of gravel riverbed and farmland habitats is one of the main causes of the high breeding density recorded in the study area. We propose that the conservation of Stone‐curlews at this site could potentially be achieved only by a synergistic management of both natural and agricultural habitats. 相似文献
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