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1.
ESR spectroscopy in the study of antigen processing--uptake of spin-labelled antigens by macrophages
Macrophages were briefly pulsed with a spin-labelled synthetic polypeptide, poly(L-tyrosine:L-glutamic acid) poly DL-alanine:poly L-lysine (n-TGAL) in the presence and absence of anti-TGAL-antibody, and the electron spin resonance (ESR) spectra of the cell suspension compared with the spectrum of free n-TGAL in solution. Spectral analysis indicated two cell-associated n-TGAL pools, one composed of freely rotating label held in an aqueous environment, susceptible to protease digestion and ascorbate reduction, and a second highly concentrated pool, sequestered intracellularly, and held within a highly ordered, polar microenvironment. The ESR analyses were completed within minutes of antigen pulsing, employed very small numbers of live cells, and did not damage the cells being tested. The utility of the technique in screening fatty acid-antigen conjugates for macrophage uptake was demonstrated. 相似文献
2.
L M Gordon F C Jensen C C Curtain P W Mobley R C Aloia 《Biochimica et biophysica acta》1988,943(2):331-342
The presence of thermodependent lipid domains in the envelope of the human immunodeficiency virus (HIV) was studied. HIV was propagated in Hut-78 cells and purified by differential-gradient centrifugation. Since the virus was highly infectious in cell culture and Western blots of detergent-inactivated HIV showed envelope proteins when exposed to sera containing anti-HIV antibodies, this viral preparation was not deficient in 'spike' or 'knob' particles. Electron spin resonance (ESR) studies of intact HIV labeled with 5-nitroxide stearate (5-NS) indicated that a temperature-dependent lipid phase separation occurs with a high onset at approx. 42 degrees C and a low onset at approx. 15 degrees C. Cooling below 42 degrees C induces 5-NS clustering. Similar phase separations with high onsets at approx. 37-38 degrees C were previously identified in 5-NS labeled human erythrocytes (cholesterol/phospholipid (C/P) molar ratio = 0.90) and cholesterol-loaded (C/P = 0.85-0.98) rat liver plasma membranes. These were attributed to a temperature-sensitive redistribution of endogenous lipid components such that 5-NS is excluded from cholesterol-rich domains and tends to reside in cholesterol-poor domains at low temperatures. Since HIV has a lipid envelope with a similarly high C/P of 0.88 (Aloia et al. (1988) Proc. Natl. Acad. Sci. USA 85, 900-904), cholesterol-rich and cholesterol-poor domains also probably exist in HIV at physiologic temperatures. The reduced stability and infectivity of HIV noted on heating above 42 degrees C may be due, in part, to the abolition of these thermodependent domains. 相似文献
3.
Summary A model has been developed for 5-nitroxide stearate, I(12,3), distribution in human erythrocyte ghosts which accurately predicts ESR spectral alterations observed with increased probe/total lipid (P/L) at 37°C. This spin probe occupies a class of high-affinity, noninteracting sites at low loading. Saturation occurs with increasing probe concentration, and, at higher loading, the probe inserts itself at initially dilute sites to form membranebound clusters of variable size. No low probe remains at high P/L where all I(12,3) clusters in a concentrated phase. This model allows determination of the dilute/clustered probe ratio, and shows that I(12,3) segregates in erythrocytes at what might otherwise be considered low P/L (e.g., 1/359). These findings validate the earlier use of empirical parameters to estimate probe sequestration in biological membranes. 相似文献
4.
The cross-reactivity of the immunoglobulin ,
1
,
2
, , , and light chain antigens of 21 ruminant species was examined using an inhibition of radioimmune precipitation technique. Although it was clear that the different antigens had evolved at different rates, the relative ranking of cross-reactivity correlated well with the accepted taxonomic order of the ruminants. It was considered significant that Antilocapra was closer to the bovids than to the cervids, in view of the disputed relationship of that genus to these families. Further, it was noted that sheep and goats appeared to be no more or less closely related than, say, the cattle and the antelopes. Of the IgG subclass antigens, IgG
1
showed the higher evolutionary stability, strong cross-reactivity being observed between sheep and deer and even between sheep and camel. This stability might be due to the conservation of sequences related to the biological properties of IgG
1
—its ability to fix complement and its secretion into the colostrum and absorption in the gut of the newborn.This work was supported in part by grants from the American Cancer Society (ET-13E) and the United States Public Health Service (AM 08527). 相似文献
5.
Eugene Giles C. C. Curtain A. Baumgarten 《American journal of physical anthropology》1967,27(1):83-88
Ten villages in or near the Markham Valley, northeastern New Guinea, have provided a sample of 476 males for the ascertainment of glucose-6-phosphate dehydrogenase (G6PD) deficiency and 810 individuals of both sexes for the investigation of the β-thalassemia trait. An extreme heterogeneity was found in the prevalence of both traits when the villages were analyzed separately (from 1.5% to 18.2% demonstrating G6PD deficiency and from 0 to 22.8% evincing β-thalassemia trait). The latter result counters an earlier report from the same region that β-thalassemia trait frequencies correlated negatively with altitude and (presumably) positively with endemicity of malaria. The present findings, based on a more representative sample, do not necessarily invalidate the relationship between malaria and β-thalassemia trait suggested by earlier studies, but they do make clear that correlation in New Guinea, at least, may be complicated by random genetic drift, although other possibilities are discussed. The heterogeneous distribution of G6PD deficiency in the present study confirms similar earlier findings. The importance of sample provenience and anthropological data in the interpretation of differences in the distribution of genetically determined traits is stressed. 相似文献
6.
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8.
Uxmal and Tulum are two important Mayan sites in the Yucatan peninsula. The buildings are mainly composed of limestone and grey/black discoloration is seen on exposed walls and copious greenish biofilms on inner walls. The principal microorganisms detected on interior walls at both Uxmal and Tulum were cyanobacteria; heterotrophic bacteria and filamentous fungi were also present. A dark‐pigmented mitosporic fungus and Bacillus cereus, both isolated from Uxmal, were shown to be acidogenic in laboratory cultures. Cyanobacteria belonging to rock‐degrading genera Synechocystis and Gloeocapsa were identified at both sites. Surface analysis previously showed that calcium ions were present in the biofilms on buildings at Uxmal and Tulum, suggesting the deposition of biosolubilized stone. Apart from their potential to degrade the substrate, the coccoid cyanobacteria supply organic nutrients for bacteria and fungi, which can produce organic acids, further increasing stone degradation. 相似文献
9.
Uptake of dietary iron is essential for replenishment of body stores. A role for the hormone gastrin in iron uptake as a chelator of ferric ions in the gastric lumen has been proposed previously [Baldwin, G. S. (1992) Med. Hypotheses 38, 70-74]. Here, spectroscopic evidence of selective, high-affinity binding of ferric ions to progastrin-derived peptides in aqueous solution at low pH is provided. The maximum at 281 nm in the absorption spectrum of glycine-extended gastrin(17) at pH 4.0 increased (2.07 +/- 0.30)-fold in the presence of > or =2 equiv of ferric ions. Titration of glycine-extended gastrin(17) with ferric ions under stoichiometric conditions indicated that the stoichiometry of binding was 2.00 +/- 0.28 mol of Fe(3+)/mol of peptide. Fluorescence quenching experiments yielded values for the stoichiometry and apparent dissociation constant of the ferric ion-glycine-extended gastrin(17) complex at pH 4.0 of 2.39 +/- 0.17 mol of Fe(3+)/mol and 0.62 +/- 0.19 microM, respectively. No interaction was detected with Co(2+), Cu(2+), Mn(2+), or Cr(3+). Electron paramagnetic resonance spectroscopy suggested that the iron ligands were either oxygen or sulfur atoms. Fluorescence quenching experiments with peptides derived from the glycine-extended gastrin(17) sequence indicated that one or more of the five glutamic acid residues were necessary for iron binding. The binding of ferric ions by glycine-extended gastrin(17) at low pH is consistent with a role for progastrin-derived peptides in iron uptake from the lumen of the gastrointestinal tract. 相似文献
10.
Novak V Yang AC Lepicovsky L Goldberger AL Lipsitz LA Peng CK 《Biomedical engineering online》2004,3(1):39