全文获取类型
收费全文 | 467篇 |
免费 | 28篇 |
出版年
2021年 | 5篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 13篇 |
2014年 | 16篇 |
2013年 | 14篇 |
2012年 | 19篇 |
2011年 | 21篇 |
2010年 | 14篇 |
2009年 | 13篇 |
2008年 | 11篇 |
2007年 | 14篇 |
2006年 | 7篇 |
2005年 | 12篇 |
2003年 | 15篇 |
2002年 | 11篇 |
2001年 | 9篇 |
2000年 | 10篇 |
1999年 | 15篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 7篇 |
1992年 | 5篇 |
1991年 | 9篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 8篇 |
1987年 | 6篇 |
1986年 | 12篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 11篇 |
1980年 | 4篇 |
1979年 | 20篇 |
1978年 | 14篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1975年 | 7篇 |
1974年 | 11篇 |
1973年 | 6篇 |
1971年 | 12篇 |
1969年 | 6篇 |
1968年 | 5篇 |
1967年 | 4篇 |
1966年 | 4篇 |
排序方式: 共有495条查询结果,搜索用时 78 毫秒
1.
In the course of studies on the oxygenation of steroids by purified P-450 cytochromes, particularly rabbit liver microsomal cytochrome P-450 form 3b, a rapid and reliable radiometric assay has been devised for progesterone 16 alpha-hydroxylation. In view of the lack of a commercially available, suitably tritiated substrate, [1,2,6,7,16,17-3H]progesterone was treated with alkali to remove the label from potential hydroxylation sites other than the 16 alpha position. The resulting [1,7,16-3H]progesterone was added to a reconstituted enzyme system containing cytochrome P-450 form 3b, NADPH-cytochrome P-450 reductase, and NADPH, and the rate of 16 alpha-hydroxylation was measured by the formation of 3H2O. This reaction was shown to be linear with respect to time and to the cytochrome P-450 concentration. An apparent tritium isotope effect of 2.1 was observed by comparison of the rates of formation of tritium oxide and 16 alpha-hydroxyprogesterone, and the magnitude of the isotope effect was confirmed by an isotope competition assay in which a mixture of [1,7,16-3H]progesterone and [4-14C]progesterone was employed. 相似文献
2.
3.
4.
5.
C E Brinckerhoff K Suzuki T I Mitchell F Oram C I Coon R D Palmiter H Nagase 《The Journal of biological chemistry》1990,265(36):22262-22269
6.
Aurora B kinase is an integral regulator of cytokinesis, as it stabilizes the intercellular canal within the midbody to ensure proper chromosomal segregation during cell division. Here we identified that the ubiquitin E3 ligase complex SCFFBXL2 mediates Aurora B ubiquitination and degradation within the midbody, which is sufficient to induce mitotic arrest and apoptosis. Three molecular acceptor sites (K102, K103 and K207) within Aurora B protein were identified as important sites for its ubiquitination. A triple Lys mutant of Aurora B (K102/103/207R) exhibited optimal resistance to SCFFBXL2-directed polyubiquitination, and overexpression of this variant resulted in a significant delay in anaphase onset, resulting in apoptosis. A unique small molecule F-box/LRR-repeat protein 2 (FBXL2) activator, BC-1258, stabilized and increased levels of FBXL2 protein that promoted Aurora B degradation, resulting in tetraploidy, mitotic arrest and apoptosis of tumorigenic cells, and profoundly inhibiting tumor formation in athymic nude mice. These findings uncover a new proteolytic mechanism targeting a key regulator of cell replication that may serve as a basis for chemotherapeutic intervention in neoplasia. 相似文献
7.
8.
9.
10.