全文获取类型
收费全文 | 18233篇 |
免费 | 1551篇 |
国内免费 | 1043篇 |
出版年
2023年 | 188篇 |
2022年 | 233篇 |
2021年 | 805篇 |
2020年 | 557篇 |
2019年 | 731篇 |
2018年 | 706篇 |
2017年 | 522篇 |
2016年 | 730篇 |
2015年 | 1104篇 |
2014年 | 1223篇 |
2013年 | 1354篇 |
2012年 | 1653篇 |
2011年 | 1501篇 |
2010年 | 945篇 |
2009年 | 843篇 |
2008年 | 968篇 |
2007年 | 889篇 |
2006年 | 751篇 |
2005年 | 655篇 |
2004年 | 595篇 |
2003年 | 494篇 |
2002年 | 442篇 |
2001年 | 372篇 |
2000年 | 304篇 |
1999年 | 281篇 |
1998年 | 169篇 |
1997年 | 162篇 |
1996年 | 169篇 |
1995年 | 125篇 |
1994年 | 131篇 |
1993年 | 101篇 |
1992年 | 156篇 |
1991年 | 116篇 |
1990年 | 95篇 |
1989年 | 87篇 |
1988年 | 76篇 |
1987年 | 81篇 |
1986年 | 80篇 |
1985年 | 56篇 |
1984年 | 60篇 |
1983年 | 52篇 |
1982年 | 29篇 |
1981年 | 18篇 |
1980年 | 23篇 |
1979年 | 23篇 |
1977年 | 16篇 |
1976年 | 19篇 |
1975年 | 19篇 |
1974年 | 16篇 |
1973年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 103 毫秒
1.
2.
3.
Residue Histidine 50 Plays a Key Role in Protecting α-Synuclein from Aggregation at Physiological pH
Ying-Chih Chi Geoffrey S. Armstrong David N. M. Jones Elan Z. Eisenmesser Chang-Wei Liu 《The Journal of biological chemistry》2014,289(22):15474-15481
α-Synuclein (αSyn) aggregation is involved in the pathogenesis of Parkinson disease (PD). Recently, substitution of histidine 50 in αSyn with a glutamine, H50Q, was identified as a new familial PD mutant. Here, nuclear magnetic resonance (NMR) studies revealed that the H50Q substitution causes an increase of the flexibility of the C-terminal region. This finding provides direct evidence that this PD-causing mutant can mediate long range effects on the sampling of αSyn conformations. In vitro aggregation assays showed that substitution of His-50 with Gln, Asp, or Ala promotes αSyn aggregation, whereas substitution with the positively charged Arg suppresses αSyn aggregation. Histidine carries a partial positive charge at neutral pH, and so our result suggests that positively charged His-50 plays a role in protecting αSyn from aggregation under physiological conditions. 相似文献
4.
5.
Jinghao Sheng Chi Luo Yuxiang Jiang Philip W. Hinds Zhengping Xu Guo-fu Hu 《The Journal of biological chemistry》2014,289(18):12520-12534
6.
Single Cell Transcriptome Amplification with MALBAC 总被引:1,自引:0,他引:1
7.
ObjectiveWe investigated whether glutamate, NMDA receptors, and eukaryote elongation factor-2 kinase (eEF-2K)/eEF-2 regulate P-glycoprotein expression, and the effects of the eEF-2K inhibitor NH125 on the expression of P-glycoprotein in rat brain microvessel endothelial cells (RBMECs).MethodsCortex was obtained from newborn Wistar rat brains. After surface vessels and meninges were removed, the pellet containing microvessels was resuspended and incubated at 37°C in culture medium. Cell viability was assessed by the MTT assay. RBMECs were identified by immunohistochemistry with anti-vWF. P-glycoprotein, phospho-eEF-2, and eEF-2 expression were determined by western blot analysis. Mdr1a gene expression was analyzed by RT-PCR.ResultsMdr1a mRNA, P-glycoprotein and phospho-eEF-2 expression increased in L-glutamate stimulated RBMECs. P-glycoprotein and phospho-eEF-2 expression were down-regulated after NH125 treatment in L-glutamate stimulated RBMECs.ConclusionseEF-2K/eEF-2 should have played an important role in the regulation of P-glycoprotein expression in RBMECs. eEF-2K inhibitor NH125 could serve as an efficacious anti-multidrug resistant agent. 相似文献
8.
9.
Yan Xue Liu Jia Wu Ke-Xin Yang Nan Pan Li-Ben Song Ying Liu Yang Tang Zhong-Hua 《Journal of Plant Growth Regulation》2022,41(6):2421-2434
Journal of Plant Growth Regulation - Early-spring plants are a special type of plant that complete their life cycle promptly in cold, early spring. Very little effort has been made into researching... 相似文献
10.