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1.
Unconventional Sequence Requirement for Viral Late Gene Core Promoters of Murine Gammaherpesvirus 68
Elaine Wong-Ho Ting-Ting Wu Zoe H. Davis Bingqing Zhang Jian Huang Hao Gong Hongyu Deng Fenyong Liu Britt Glaunsinger Ren Sun 《Journal of virology》2014,88(6):3411-3422
Infection with the human gammaherpesviruses, Epstein-Barr virus (EBV) and Kaposi''s sarcoma-associated herpesvirus (KSHV), is associated with several cancers. During lytic replication of herpesviruses, viral genes are expressed in an ordered cascade. However, the mechanism by which late gene expression is regulated has not been well characterized in gammaherpesviruses. In this study, we have investigated the cis element that mediates late gene expression during de novo lytic infection with murine gammaherpesvirus 68 (MHV-68). A reporter system was established and used to assess the activity of viral late gene promoters upon infection with MHV-68. It was found that the viral origin of lytic replication, orilyt, must be on the reporter plasmid to support activation of the late gene promoter. Furthermore, the DNA sequence required for the activation of late gene promoters was mapped to a core element containing a distinct TATT box and its neighboring sequences. The critical nucleotides of the TATT box region were determined by systematic mutagenesis in the reporter system, and the significance of these nucleotides was confirmed in the context of the viral genome. In addition, EBV and KSHV late gene core promoters could be activated by MHV-68 lytic replication, indicating that the mechanisms controlling late gene expression are conserved among gammaherpesviruses. Therefore, our results on MHV-68 establish a solid foundation for mechanistic studies of late gene regulation. 相似文献
2.
T.‐J. Qin J. Zhou Y. Sun H. Müller‐Schrer F.‐L. Luo B.‐C. Dong H.‐L. Li F.‐H. Yu 《Plant biology (Stuttgart, Germany)》2020,22(4):591-599
- Highly biodiversity communities have been shown to better resist plant invasions through complementarity effects. Species richness (SR) is a widely used biodiversity metric but lacks explanatory power when there are only a few species. Communities with low SR can have a wide variety of phylogenetic diversities (PD), which might allow for a better prediction of invasibility.
- We assessed the effect of diversity reduction of a wetland community assemblage typical of the Beijing area on biotic resistance to invasion of the exotic weed Alternanthera philoxeroides and compared the reduction in SR and PD in predicting community invasibility.
- The eight studied resident species performed similarly when grown alone and when grown in eight‐species communities together with the invasive A. philoxeroides. Variation partitioning showed that PD contributed more to variation in both A. philoxeroides traits and community indicators than SR. All A. philoxeroides traits and community indicators, except for evenness index, showed a linear relationship with PD. However, only stem length of A. philoxeroides differed between the one‐ and two‐species treatments, and the diversity index of the communities differed between the one‐ and two‐species treatments and between the one‐ and four‐species treatments.
- Our results showed that in natural or semi‐natural wetlands with relatively low SR, PD may be a better predictor of invasibility than SR. When designing management strategies for mitigating A. philoxeroides invasion, deliberately raising PD is expected to be more efficient than simply increasing species number.
3.
Yun-gang Luo Ping Liu Lin Shi Yishan Luo Lei Yi Ang Li Jing Qin Pheng-Ann Heng Defeng Wang 《PloS one》2015,10(9)
Neuroimage registration is crucial for brain morphometric analysis and treatment efficacy evaluation. However, existing advanced registration algorithms such as FLIRT and ANTs are not efficient enough for clinical use. In this paper, a GPU implementation of FLIRT with the correlation ratio (CR) as the similarity metric and a GPU accelerated correlation coefficient (CC) calculation for the symmetric diffeomorphic registration of ANTs have been developed. The comparison with their corresponding original tools shows that our accelerated algorithms can greatly outperform the original algorithm in terms of computational efficiency. This paper demonstrates the great potential of applying these registration tools in clinical applications. 相似文献
4.
植物叶片功能性状能够响应环境条件的变化,反应了植物对环境的适应策略。当前,针对藤本植物叶片功能性状地理格局及其环境驱动力的研究较少。以国家重点保护植物永瓣藤(Monimopetalum chinense)为研究对象,对其分布区内11个种群的15个叶片功能性状进行测量,并结合气候、土壤因子来解释叶性状变异。比较叶片性状在局域和区域尺度上的种内变异程度,利用多元逐步回归分析环境因子对叶性状的影响。结果表明,在局域尺度上,永瓣藤叶功能性状变异系数介于3.0%-22.5%,其中,叶面积变异程度最大,叶片碳含量变异最小。永瓣藤叶片形状随纬度上升而变得宽且圆。叶片磷含量相对较低,永瓣藤的生长可能受到了磷限制。土壤与气候因子是叶片性状的重要驱动因素,解释了25%-97%的叶片性状变异。在温度和水分充足的情况下,永瓣藤叶片趋向于的慢速生长的保守策略。总体来说,永瓣藤叶片功能性状通过一定的种内变异和性状组合,并与气候、土壤因子相互作用,适应当前的环境条件。 相似文献
5.
6.
Su Hao Lo Ellen Weisberg Lan Bo Chen 《BioEssays : news and reviews in molecular, cellular and developmental biology》1994,16(11):817-823
Cytoskeletal proteins provide the structural foundation that allows cells to exist in a highly organized manner. Recent evidence suggests that certain cytoskeletal proteins not only maintain structural integrity, but might also be associated with signal transduction and suppression of tumorigenesis. Since the time of the discovery of tensin, a fair amount of data has been gathered which supports the notion that tensin is one such protein possessing these characteristics. In this review, we discuss recent studies that: (1) elucidate a role for tensin in maintenance of cellular structure and signal transduction; (2) implicate tensin as the anchor for actin filaments at the focal adhesion; (3) describe the phosphorylation of tensin; (4) describe potential targets for its Src homology region 2 domain; (5) describe the association between tensin and the nuclear protein p130; and (6) demonstrate that increased tensin expression in a cell line appears to reduce its transformation potential. 相似文献
7.
8.
【目的】根结线虫危害严重,难防难控,前期研究发现一株深海来源的独岛枝芽胞杆菌(Virgibacillus dokdonensis) MCCC 1A00493对南方根结线虫具有良好的体外拮抗效果,本研究分离鉴定菌株发酵液中杀线虫活性物质,对其作用线虫的多种模式进行研究,为菌株有效控制植物病原线虫的应用奠定理论基础。【方法】采用硅胶柱层析和半制备高效液相色谱对独岛枝芽胞杆菌发酵上清液中的杀线虫活性物质进行分离纯化,采用液相色谱质谱联用、核磁共振对纯化物进行结构鉴定;并对其趋避、诱杀、熏杀和卵孵化抑制活性进行检测。【结果】结构鉴定确定独岛枝芽胞杆菌MCCC 1A00493发酵液中分离的杀线虫活性物质为4-乙烯基苯酚。4-乙烯基苯酚对线虫具有触杀、熏杀、卵孵化抑制和高浓度驱避低浓度引诱活性,15μg/mL 4-乙烯基苯酚72 h触杀线虫校正死亡率为71.23%±9.06%;20 mg/mL 4-乙烯基苯酚24 h熏杀线虫死亡率达100%;100μg/mL4-乙烯基苯酚作用线虫卵10d后,卵孵化抑制率达66.2%;在琼脂平板上,10mg/mL的4-乙烯基苯酚对线虫有驱避作用,1 mg/mL的4-... 相似文献
9.
C. Lu Z. Xie F. Yu L. Tian X. Hao X. Wang L. Chen D. Li 《Plant biology (Stuttgart, Germany)》2020,22(4):655-667
- Mitochondrial function is critical for cell vitality in all eukaryotes including plants. Although plant mitochondria contain many proteins, few have been studied in the context of plant development and physiology.
- We used knock‐down mutant RPS9M to study its important role in male gametogenesis and seed development in Arabidopsis thaliana.
- Knock‐down of RPS9M in the rps9m‐3 mutant led to abnormal pollen development and impaired pollen tube growth. In addition, both embryo and endosperm development were affected. Phenotype analysis revealed that the rps9m‐3 mutant contained a lower amount of endosperm and nuclear proteins, and both embryo cell division and embryo pattern were affected, resulting in an abnormal and defective embryo. Lowering the level of RPS9M in rps9m‐3 affects mitochondrial ribosome biogenesis, energy metabolism and production of ROS.
- Our data revealed that RPS9M plays important roles in normal gametophyte development and seed formation, possibly by sustaining mitochondrial function.
10.