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排序方式: 共有146条查询结果,搜索用时 15 毫秒
1.
The lipid composition of swimming spores, cysts and five hour germlings was established. Spores utilized triglycerides first, then phospholipids. Upon encystment all glycolipid components decreased, while in germlings the phospholipids, monoglycerides and sterol esters exhibited a marked increase. 相似文献
2.
M R Celio A Poncino D Cantino 《Bollettino della Società italiana di biologia sperimentale》1992,68(1):25-29
Calcium-binding proteins are present in different neuron populations in the Central Nervous System. As concerns the Enteric Nervous System, only a few studies have been performed. In the present work we investigated immunohistochemically the localization of Calretinin in neurons of the human intestinal wall. Our results showed the presence of stained cell bodies and fibers with antibodies against Calretinin in the Auerbach plexus. Since most of the enteric neurons are characterized by a slow phase after hyperpolarization caused by Ca2+ dependent K+ channels, the role of Calretinin could be to modulate this particular electrophysiological behaviour. 相似文献
3.
PD Dr. G. F. Jirikowski J. F. Ramalho-Ortigao K. W. Kesse F. E. Bloom 《Histochemistry and cell biology》1990,94(2):187-190
Summary We recently described a nonradioactive method for in situ hybridization with 5-bromo-2-deoxyuridine (BrdU) labelled oligonucleotide
probes. An antibody to BrdU and immunocytochemistry were used in order to detect the hybridization signal. We have now applied
this method to semithin Epon sections, in order to hybridize consecutive sections through single cells with different probes
and to stain them with antibodies to neuropeptides. It could be shown that Epon embedding preserves mRNA well. In the present
study we used a BrdU labelled synthetic oligonucleotide probe complementary to a fragment of the vasopressin precursor and
an antibody to Arg-vasopressin. Vasopressin mRNA was demonstrable in a fraction of the vasopressin immunoreactive neurons
in the magnocellular nuclei. In addition some of the magnocellular neurons showed either hybridization or vasopressin immunostaining
only, perhaps indicating different stages of synthetic and secretory activity.
The method described seems to be a valuable tool for studying synthetic activity in peptidergic neurons on a single cell level.
The method might also have potential for in situ hybridization on the electronmicroscopical level. 相似文献
4.
Kenneth T. Izutsu Mark K. Goddard Jeanne M. Iversen Murray R. Robinovitch Teresa K. Oswald Marie Cantino Dale Johnson 《Cell and tissue research》1991,263(3):535-540
Summary The relationship between granule density, protein content, and Ca and S contents were studied in two secretory granule fractions, from parotid glands of the rat, previously shown to constitute different stages in granule maturation. The density of the lighter fraction was between 1.133 and 1.142 g/ml, while that of the heavier fraction was greater than 1.142 g/ml. The mean protein content of the denser granules was 12% greater than that of the lighter granules (P<0.03), while the dry-mass elemental concentrations in the two granule fractions were unchanged. These results indicate that protein is added to granules during the maturation process (presumably by vesicular traffic), and that the resulting increase in granule density is not driven simply by decrease in water content and/or increased concentrations of inorganic Ca or S in the granules. The elemental concentration values also indicate that the diffusible elements permeate the granule membrane during the fractionation procedures. 相似文献
5.
Partial purification and properties of D-glucosamine 6'-phosphate N-acetyltransferase from zoospores of Blastocladiella emersonii 总被引:2,自引:0,他引:2 下载免费PDF全文
A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. 相似文献
6.
In an effort to obtain orange mutants ofBlastocladiella emersonii
Cantino &Hyatt, wild type zoospores were treated with mitomycin. From the variants produced, we obtained a stable, albino mutant (Ma-1) that differs significantly from another, previously described (Shaw &Cantino, 1969) UV-induced, albino variant. This report concerns the origin of Ma-1, its distinguishing features, and its apparent similarity to the few LC (late colorless) plants that normally appear in wild type populations. A preliminary note regarding mitomycin-induced variants ofB. emersonii has been published (Matsumae &Cantino, 1970). 相似文献
7.
8.
Summary The microproblematicumPycnoporidium ? eomesozoicum
Flügel, 1972, from Upper Triassic reefs of the Alpine-Mediterranean region, Turkey Oman and Iran (originally interpreted as possible
alga) represents the type species of a new strophomenid brachiopod genus (Gosaukammerella n.g.). The genus is characterized by a very small, millimeter-sized plano-convex shell, whose ventral valve is attached to
the substratum (mainly sponges) by symmetrically arranged outgrowths developing from a pseudopunctate, lamellose foliated
shell wall and composed of densely spaced subparallel ‘tubes’ comparable with productide spines secreted by papillose extensions
of the mantle.Gosaukammerella seems to be the only reliable candidate for the existence of post-Paleozoic strophomenid (productid ?) brachiopods.
Gosaukammerella eomesozoica is restricted to possibly cryptic, shaded reef environments inhabited predominantly by sponges serving as substrates for
micromorphic brachiopods. 相似文献
9.
Summary Intercellular junctions in the uterine epithelium of the ovoviviparous urodele Salamandra salamandra were studied in pregnant and non-pregnant females by freeze-fracture technique. Junctional complexes consist of zonulae occludentes (tight junctions) and numerous maculae adhaerentes (desmosomes); z. adhaerentes and nexuses (gap junctions) could not be identified. Tight junctions are of the flexible type exhibiting loosely interconnected fibrils. The fibrillary network appears stretched more often in pregnant females possibly due to the mechanical stress of pregnancy. The structure and occurrence of the junctions identified, especially that of the tight junctions, is discussed with regard to the functions of the uterus during pregnancy.Zusammenfassung Mit Hilfe der Gefrierbruchtechnik wurden im Uterus-epithel trächtiger und nichtträchtiger Feuersalamanderweibchen (Salamandra salamandra) Zonulae occludentes und Maculae adhaerentes, jedoch keine Z. adhaerentes sowie Nexus identifiziert. Die Z. occludentes sind flexibel. Ihr fibrilläres Netzwerk ist bei trächtigen Weibchen öfter gestreckt; das ist möglicherweise auf die stärkere Ausdehnung des Uterusgewebes während der Trächtigkeit zurückzuführen. Das Vorkommen der verschiedenen Kontakt-strukturen, namentlich das der Z. occludentes, wird im Hinblick auf die Funktionen des Uterus während der Trächtigkeit diskutiert.We are indebted to Mrs. K. Ott for excellent technical assistance and to Miss Dr. U. Beigel for linguistic help 相似文献
10.