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DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. 总被引:15,自引:6,他引:9 下载免费PDF全文
J L Van Etten A M Schuster L Girton D E Burbank D Swinton S Hattman 《Nucleic acids research》1985,13(10):3471-3478
The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six of the viral DNAs also contain N6-methyldeoxyadenosine. At least some of the methylated bases in the host reside in different base sequences than the methylated bases in the viruses as shown by differential susceptibility to restriction endonuclease enzymes. This suggests that the viruses encode for DNA methyltransferases with sequence specificities different from that of the host enzyme. 相似文献
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Abarca-Grau AM Burbank LP de Paz HD Crespo-Rivas JC Marco-Noales E López MM Vinardell JM von Bodman SB Penyalver R 《Applied and environmental microbiology》2012,78(6):1644-1651
Rhizobium rhizogenes strain K84 is a commercial biocontrol agent used worldwide to control crown gall disease. The organism binds tightly to polypropylene substrate and efficiently colonizes root surfaces as complex, multilayered biofilms. A genetic screen identified two mutants in which these surface interactions were affected. One of these mutants failed to attach and form biofilms on the abiotic surface although, interestingly, it exhibited normal biofilm formation on the biological root tip surface. This mutant is disrupted in a wcbD ortholog gene, which is part of a large locus predicted to encode functions for the biosynthesis and export of a group II capsular polysaccharide (CPS). Expression of a functional copy of wcbD in the mutant background restored the ability of the bacteria to attach and form normal biofilms on the abiotic surface. The second identified mutant attached and formed visibly denser biofilms on both abiotic and root tip surfaces. This mutant is disrupted in the rkpK gene, which is predicted to encode a UDP-glucose 6-dehydrogenase required for O-antigen lipopolysaccharide (LPS) and K-antigen capsular polysaccharide (KPS) biosynthesis in rhizobia. The rkpK mutant from strain K84 was deficient in O-antigen synthesis and exclusively produced rough LPS. We also show that strain K84 does not synthesize the KPS typical of some other rhizobia strains. In addition, we identified a putative type II CPS, distinct from KPS, that mediates cell-surface interactions, and we show that O antigen of strain K84 is necessary for normal cell-cell interactions in the biofilms. 相似文献
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Victoria Burbank 《The Australian journal of anthropology》2014,25(1):1-21
The word ‘envy’ directs attention to feelings and cognitions that are especially important sources of information in our complicated sociality. As it is delimited by philosophers, economists, psychologists and others, envy is conceptually nested within a family that includes evil eye beliefs, inequity aversion, strong reciprocity and social comparison. Although the accumulation of work in these areas is substantial, anthropological treatments of envy are rare. Given repeated assertions of envy's universality and its potential importance for understanding widespread aspects of the human condition, a comparative eye seems essential. I present an account of ‘jealous’ in Aboriginal Australia via a framework that casts emotions as emerging from the interaction of psychobiological and sociocultural processes. According to this perspective, ‘envy’ should not be regarded as an invariant human condition but rather as a Western version of what, in a more generic human form, may both defend the individual and the larger sociality. 相似文献
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Qiudeng Que Yanping Zhang Michael Nelson Susan Ropp Dwight E. Burbank James L. Van Etten 《Gene》1997,190(2):25-244
Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M- CviSI (TGCmA), M· CviSII CmATG), M· CviSIII (TCGmA) and MmCviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M· CviSV (RCmCG), and one nonfunctional m5C MTase, M· CviSVI, which is homologous to the MTase M· CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M· CviSII, and M· CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M· CviSI nor M· CviSIII genes hybridized to genes for their respective isomethylomers, M· CviRI and M· CviBIII, from other chlorella viruses. However, the M· CviSII gene hybridized strongly to its M· CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome. 相似文献
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Plaque-forming viruses of the unicellular, eucaryotic, exsymbiotic, Chlorella-like green algae strain NC64A, which are common in the United States, were also present in fresh water collected in the People's Republic of China. Seven of the Chinese viruses were examined in detail and compared with the Chlorella viruses previously isolated in the United States. Like the American viruses, the Chinese viruses were large polyhedra and sensitive to chloroform. They contained numerous structural proteins and large double-stranded DNA genomes of at least 300 kilobase pairs. Each of the DNAs from the Chinese viruses contained 5-methyldeoxycytosine, which varied from 12.6 to 46.7% of the deoxycytosine, and N6-methyldeoxyadenosine, which varied from 2.2 to 28.3% of the deoxyadenosine. Four of the Chinese virus DNAs hybridized extensively with DNA from the American virus PBCV-1, and three hybridized poorly. 相似文献
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Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga. 总被引:14,自引:1,他引:13 下载免费PDF全文
Y N Xia D E Burbank L Uher D Rabussay J L Van Etten 《Molecular and cellular biology》1986,6(5):1430-1439
An enzyme was isolated from a eucaryotic, Chlorella-like green alga infected with the virus PBCV-1 which exhibits type II restriction endonuclease activity. The enzyme recognized the sequence GATC and cleaved DNA 5' to the G. Methylation of deoxyadenosine in the GATC sequence inhibited enzyme activity. In vitro the enzyme cleaved host Chlorella nuclear DNA but not viral DNA because host DNA contains GATC and PBCV-1 DNA contains GmATC sequences. PBCV-1 DNA is probably methylated in vivo by the PBCV-1-induced methyltransferase described elsewhere (Y. Xia and J. L. Van Etten, Mol. Cell. Biol. 6:1440-1445). Restriction endonuclease activity was first detected 30 to 60 min after viral infection; the appearance of enzyme activity required de novo protein synthesis, and the enzyme is probably virus encoded. Appearance of enzyme activity coincided with the onset of host DNA degradation after PBCV-1 infection. We propose that the PBCV-1-induced restriction endonuclease participates in host DNA degradation and is part of a virus-induced restriction and modification system in PBCV-1-infected Chlorella cells. 相似文献
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Alluvial sequence in the north piedmont of the Chinese Tian Shan over the past 550 kyr and its relationship to climate change 总被引:1,自引:0,他引:1
Honghua Lu Douglas W. Burbank Youli Li 《Palaeogeography, Palaeoclimatology, Palaeoecology》2010,285(3-4):343-353
A new division of Middle and Late Pleistocene alluvial sequence in the north piedmont of the Chinese Tian Shan based on geomorphologic, stratigraphic, and chronologic criteria provides a framework for examining their relationship to climate change during glacial–interglacial transitions. Over the past 550 kyr at least four major alluviation episodes occurred within the piedmont. Along the major river valleys in this region, each episode of alluvial fan deposition morphologically correlates with a major river terrace. These correlations create a regionally applicable framework for subdivision of the Quaternary alluvial sequence in the study area, where seven stepped river terraces are defined. Our new chronology of this fluvial sequence suggests that, following intervals of aggradation, three highest river terraces and equivalent alluvial fans were abandoned at ~ 530 ka, ~ 300 ka and ~ 10 ka, respectively. Paleosols at the base of the loess sequences that directly overlie the older terraces and fans suggest that episodes of aggradation occurred late in the glacial cycles. The subsequent incision that caused abandonment/stabilization of these terraces and fans occurred near to glacial–interglacial transitions. A relatively high degree of synchrony in major river incision events across the piedmont, despite disruption by several discrete structure zones with asychronous tectonic activities, supports the dominant control exerted by climatic conditions on alluvial deposition and terrace creation during the Quaternary across the north piedmont of the Chinese Tian Shan. 相似文献