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Boronnikova SV Kokaeva ZG Gostimskiĭ SA Dribnokhodova OP Tikhomirova NN 《Genetika》2007,43(5):653-659
Genetic polymorphism of the Uralian relict plant species, yellow foxglove Digitalis grandiflora Mill. (family Scrophulariaceae), was examined using RAPD and ISSR techniques. A total of 149 RAPD and 74 ISSR markers were tested. The indices characterizing polymorphism and genetic diversity were calculated. The data obtained pointed to a high level of genetic variation of D. grandiflora (P95 = 65%). The cenopopulation examined was weakly differentiated with most of genetic diversity accounted by within-population differentiation. 相似文献
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S. V. Boronnikova Z. G. Kokaeva S. A. Gostimsky O. P. Dribnokhodova N. N. Tikhomirova 《Russian Journal of Genetics》2007,43(5):530-535
Genetic polymorphism of the Uralian relict plant species, large-flowered foxglove Digitalis grandiflora Mill. (family Scrophulariaceae), was examined using RAPD and ISSR techniques. A total of 149 RAPD and 74ISSR markers were tested. The indices characterizing polymorphism and genetic diversity were calculated. The data obtained pointed to a high level of genetic variation of D. grandiflora (P 95 = 65%). The cenopopulation examined was weakly differentiated with most of genetic diversity accounted by within-population differentiation. 相似文献
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Yu. S. Nechaeva A. A. Julanov S. V. Boronnikova Ya. V. Prishnivskaya 《Russian Journal of Genetics》2017,53(5):587-595
The objectives of conservation and sustainable forest management require in depth study of genomes of woody plants and definition of their intraspecific genetic diversity. In recent years, an approach was developed based on the study of “candidate genes” that can potentially be involved in the formation of adaptive traits. In this study, we investigated nucleotide polymorphism of several adaptive candidate genes in the populations of Siberian larch (Larix sibirica Ledeb.) in the Urals. Representatives of this genus are among the most valuable and widely distributed forest tree species in Russia. From ten selected gene loci in the genome of L. sibirica, we isolated and investigated three loci, one of which (ABA-WDS) was sequenced in L. sibirica for the first time. The total length of the analyzed sequence in each individual amounted to 2865 bp. The length of locus alignment was from 360 bp to 1395 bp. In total, we identified 200 polymorphic positions. The most conservative is locus 4CL1-363, and the most polymorphic is locus sSPcDFD040B03103-274. The studied populations of L. sibirica are characterized by a high level of nucleotide polymorphism in comparison with other species and genuses (Picea, Pinus, Pseudotsuga, Abies) conifers plants (Hd = 0.896; π = 0.007; θW = 0.015). The most selectively neutral polymorphism (D T =–0.997) was attributed to locus 4CL1-363, and polymorphism with high probability of adaptability (D T =–1.807) was determined for the ABA-WDS locus. We identified 54 SNP markers, only five of which were nonsynonymous (9.26%) replacements. The average frequency of SNPs in the three studied loci of L. sibirica was one SNP in 53 bp. We detected unique SNP markers for eight populations, which could potentially be used to identify populations. Populations that are characterized by the highest number of unique SNP markers can be recommended for selection in order to preserve the gene pool of the species. 相似文献
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S. V. Boronnikova 《Russian Journal of Genetics》2009,45(5):571-574
The genetic variation in four populations of Adenophora lilifolia (L.) DC., a rare plant species of the Perm region, was analyzed using 56 ISSR markers. The characteristics of DNA polymorphism and population genetic diversity were determined. These data demonstrate a high level of DNA polymorphism (P 95 = 82.14%). The studied A. lilifolia populations are weakly differentiated; the intrapopulation variation is the main contributor to the genetic variation. 相似文献
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Species-specific LTR retrotransposons were first cloned in five rare relic species of drug plants located in the Perm’ region.
Sequences of LTR retrotransposons were used for PCR analysis based on amplification of repeated sequences from LTR or other
sites of retrotransposons (IRAP). Genetic diversity was studied in six populations of rare relic species of plants Adonis vernalis L. by means of the IRAP method; 125 polymorphic IRAP-markers were analyzed. Parameters for DNA polymorphism and genetic diversity
of A. vernalis populations were determined. 相似文献
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Shumkova E. S. Egorova D. O. Boronnikova S. V. Plotnikova E. G. 《Molecular Biology》2015,49(4):569-580
Molecular Biology - Polychlorinated biphenyls (PCBs) are persistent organic pollutants. Biphenyl 2,3-dioxygenase (BDO) is a key enzyme that determines the range of PCBs oxidized by a bacterial... 相似文献
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