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1.
Arachidonic acid metabolites are mediators of various pathophysiologic events following endotoxin administration. However, their role in the endotoxin-induced increase in glucose metabolism has not been examined. Rats were administered either saline or BW755C (an inhibitor of both the cyclooxygenase and lipoxygenase pathways) 30 min prior to injection of E. coli endotoxin and whole body glucose kinetics assessed using a constant iv infusion of [6-3H] glucose. Treatment with BW755C prevented the endotoxin-induced hypotension and tachycardia. Endotoxin produced characteristic increases in the plasma glucose (23-70%) and lactate (2- to 9-fold) concentrations, as well as elevations in the rate of glucose appearance (34-63%) and metabolic clearance (40-92%). In contrast to the amelioration in hemodynamics, pretreatment with BW755C did not prevent these alterations in glucose metabolism normally seen after endotoxin. BW755C markedly reduced the endotoxin-induced increase in plasma catecholamine concentrations, but levels were still elevated 2- to 4-fold compared to control values. The results suggest that arachidonic acid metabolites mediate the early hypotensive response following endotoxin, but are not by themselves responsible for the elevated rates of glucose production and utilization. 相似文献
2.
Embryogenic tissue of the sweet potato (Ipomoea batatas (L) LAM) genotype TIB 10 was established from in vitro axillary shoot tips on Murashige and Skoog (1962) medium supplemented with 5 M 2,4-dichlorophenoxyacetic acid. Embryogenic aggregates of fresh mass 9.0–12 mg were subjected to a rapid freezing protocol in liquid nitrogen following sucrose preculture and varying degrees of dehydration. Up to 50% of embryogenic explants survived rapid freezing after preculture on 0.4 or 0.7M sucrose only. Dehydration with silica gel to moisture contents in the range 18–41% improved the survival after cryopreservation of embryogenic tissue. Tissue dehydrated for intermediate periods exhibited poor survival. Following freezing, embryogenic tissue appeared to develop normally, retaining its competence to produce mature embryos and plantlets.Abbreviations BA
6-benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- MS
Murashige and Skoog (1962) medium 相似文献
3.
Shoot regeneration from mature endosperm of Passiflora foetida 总被引:1,自引:0,他引:1
Murashige and Skoog (1962) medium supplemented with 2 M 6-benzylaminopurine (BA) induced adventitious shoots on mature endosperm explants, whilst gibberellic acid (GA3) and casein hydrolysate stimulated growth and development of these shoot primordia. Plantlets were successfully weaned in vivo. These plants were found to be triploid and flowered, although fruit set was not observed. 相似文献
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5.
E. J. Buck M. Hadonou C. J. James D. Blakesley K. Russell 《Molecular ecology resources》2003,3(2):239-241
Thirteen polymorphic microsatellite loci were isolated from Castanea sativa (Mill.). Six contained dinucleotide repeats, six contained trinucleotide repeats, and one contained a compound microsatellite of a trinucleotide and a tetranucleotide repeat. The loci were characterized using C. sativa trees from three populations in the UK and the parents and six seedlings from a Turkish mapping population. The number of alleles revealed varied from two to 14 (mean = 5.15) per loci. Eight loci were found to be useful in the mapping family. 相似文献
6.
S. Al-Mazrooei M. H. Bhatti G. G. Henshaw N. J. Taylor D. Blakesley 《Plant cell reports》1997,16(10):710-714
Culture procedures have been developed to facilitate the induction and maintenance of somatic embryogenic tissues in 14 out
of 16 tested cultivars of sweet potato [Ipomoea batatas (L.) Lam]. Both the size of the axillary bud explant and the type of auxin were found to be critical for the successful induction
of somatic embryogenesis. Of the five auxins screened 2,4-dichlorophenoxyacetic acid 2,4-D and 2,4,5-trichlorophenoxyacetic
acid were the most effective, with use of the latter inducing the production of embryogenic tissues in 7 cultivars which responded
poorly or not at all to 2,4-D. Procedures for secondary/cyclic embryogenesis, formation of mature embryos and their conversion
to plants are also described.
Received: 24 September 1996 / Revision received: 16 December 1996 / Accepted 27 January 1997 相似文献
7.
M. H. Bhatti T. Percival C. D. M. Davey G. G. Henshaw D. Blakesley 《Plant cell reports》1997,16(11):802-806
Embryogenic tissue of nine sweet potato [Ipomoea batatas (L.) Lam] genotypes from Asia, Africa and the Americas was established from in vitro axillary buds on Murashige and Skoog
medium supplemented with 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. Embryogenic aggregates, 1.0–2.0
mm in diameter, were encapsulated in alginate gel, precultured on medium containing elevated levels of sucrose and dehydrated
prior to rapid freezing in liquid nitrogen. The maximum survival of embryogenic tissue ranged from 4% to 38%, depending on
the genotype. With the incorporation of a slow-cooling step, survival was generally much higher than that obtained after rapid
freezing alone. Five of eight genotypes tested with this protocol gave survival percentages in excess of 55%, and a further
two in excess of 33%, all after evaporative dehydration. The most effective sucrose treatment(s), however, varied with the
genotype.
Received: 7 October 1996 / Revision received: 16 December 1996 / Accepted 27 January 1997 相似文献
8.
John A. Boezi Lucy F. Lee Robert W. Blakesley Mark Koenig Howard C. Towle 《Journal of virology》1974,14(5):1209-1219
9.
Claxton J. R. Arnold D. L. Clarkson J. M. Blakesley D. 《Plant Cell, Tissue and Organ Culture》1998,52(3):155-164
A range of watercress (Rorippa nasturtium-aquaticum) explants (stems, hypocotyls, true-leaves, cotyledons and petioles) were
tested for their capacity to regenerate adventitious shoots from callus formed using Murashige and Skoog medium containing
different concentrations of thidiazuron and 2,4-dichlorophenoxyacetic acid. The highest shoot regeneration rate was a mean
of 18 shoots per responding explant from stem callus formed on medium containing 5 μM thidiazuron and 0.05 μM 2,4-dichlorophenoxyacetic
acid. A histological study confirmed that shoots originated directly from callus tissue. Twenty five percent of somaclones
exhibited somaclonal variation in leaf shape, plant height, axillary branching or ploidy. The variation in 6% of somaclones
was heritable to the first selfed generation. A screening protocol was developed to permit the identification of somaclones
with increased resistance to the economically damaging watercress root pathogen, Spongospora subterranea f. sp. nasturtii.
Although 883 somaclones were screened using this protocol, no significant increase in disease resistance was detected.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
10.