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1.
Medine Gulluce Tugba Bal Hakan Ozkan Ahmet Adiguzel Fikrettin Sahin Derya Yanmis 《Geomicrobiology journal》2014,31(5):445-451
In this study, the bacteria having ore enrichment potential were isolated from three different magnesite quarries located in Erzurum-Askale borderlines. The obtained isolates were identified and characterized according to the conventional (morphological, physiological and biochemical tests) and molecular techniques (fatty acid methyl ester profiles (FAME), BOX PCR and 16S rDNA). According to sequence analysis, they were determined as Exiguobacterium aurantiacum (4), Exiguobacterium sibiricum (2), Bacillus sp. (2), Staphylococcus epidermidis (2), Staphylococcus haemolyticus (1), Shewanella baltica (1) and Klebsiella oxytoca (1), respectively. 相似文献
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Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position 131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way for the mass production of such vaccines. 相似文献
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E. Balátová-Tuláčková 《Plant Ecology》1985,59(1-3):111-117
Forty Molinietalia communities were analyzed from the phytogeographical point of view in order to establish their distribution tendencies in Czechoslovakia in relation to the floristic regions of Hercynicum, Pannonicum and Carpaticum. Only few moist meadow communities of the Molinietalia order are found in the whole area under study; most of them have their distribution center in one of the floristic regions mentioned above. Eleven Calthion and two Molinion associations are confined to the Hercynicum, eight of them strictly. It is only in the Moravian and Slovak Pannonicum that all associations of the alliances Cnidion venosi and Veronico longifoliae-Lysimachion occur; they appear there together with two Molinion communities, the distribution of which is, however, a little wider. Three Calthenion communities show a still wider distribution, extending into the adjoining areas. One Calthenion community occurs in the Bohemian Pannonicum and its surroundings, two Calthenion associations have their distribution center in the Carpaticum, but they are not strictly confined to it.The author wants to express her thanks to Doc. Dr Miroslav Smejkal, CSc., for critical revision of the text. 相似文献
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Naresh C. Bal Ashoke Sharon Subash C. Gupta Nivedita Jena Sana Shaikh Sandor Gyorke Muthu Periasamy 《The Journal of biological chemistry》2010,285(22):17188-17196
Calsequestrin undergoes dynamic polymerization with increasing calcium concentration by front-to-front dimerization and back-to-back packing, forming wire-shaped structures. A recent finding that point mutation R33Q leads to lethal catecholaminergic polymorphic ventricular tachycardia (CPVT) implies a crucial role for the N terminus. In this study, we demonstrate that this mutation resides in a highly conserved alternately charged residue cluster (DGKDR; cluster 1) in the N-terminal end of calsequestrin. We further show that this cluster configures itself as a ring system and that the dipolar arrangement within the cluster brings about a critical conformational flip of Lys31-Asp32 essential for dimer stabilization by formation of a H-bond network. We additionally show that Ca2+-induced calsequestrin aggregation is nonlinear and reversible and can regain the native conformation by Ca2+ chelation with EGTA. This study suggests that cluster 1 works as a molecular switch and governs the bidirectional transition between the CASQ2 monomer and dimer. We further demonstrate that mutations disrupting the alternating charge pattern of the cluster, including R33Q, impair Ca2+-CASQ2 interaction, leading to altered polymerization-depolymerization dynamics. This study provides new mechanistic insight into the functional effects of the R33Q mutation and its potential role in CPVT. 相似文献
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Janne Alahuhta Sarian Kosten Munemitsu Akasaka Dominique Auderset Mattia M. Azzella Rossano Bolpagni Claudia P. Bove Patricia A. Chambers Eglantine Chappuis John Clayton Mary de Winton Frauke Ecke Esperança Gacia Gana Gecheva Patrick Grillas Jennifer Hauxwell Seppo Hellsten Jan Hjort Mark V. Hoyer Christiane Ilg Agnieszka Kolada Minna Kuoppala Torben Lauridsen En Hua Li Balázs A. Lukács Marit Mjelde Alison Mikulyuk Roger P. Mormul Jun Nishihiro Beat Oertli Laila Rhazi Mouhssine Rhazi Laura Sass Christine Schranz Martin Søndergaard Takashi Yamanouchi Qing Yu Haijun Wang Nigel Willby Xiao Ke Zhang Jani Heino 《Journal of Biogeography》2017,44(8):1758-1769
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A set of scaling feedforward filters is developed in an unsupervised way via inputting pixel-discretized extended objects into a winner-take-all artificial neural network. The system discretizes the input space by both position and size. Depending on the distribution of input samples and below a certain number of neurons the spatial filters may form groups of similar filter sizes with each group covering the whole input space in a quasi-uniform fashion. Thus a multi-discretizing system may be formed. Interneural connections of scaling filters are also developed with the help of extended objects. It is shown both theoretically and with the help of numerical simulation that competitive Hebbian learning is suitable for defining neighbours for the multi-discretizing system. Taking into account the neighbouring connections between filters of similar sizes only, i.e. within the groups of filters, the system may be considered as a self-organizing multi-grid system. 相似文献
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