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Conventional and Molecular Identification of Bacteria with Magnesite Enrichment Potential from Local Quarries in Erzurum
Authors:Medine Gulluce  Tugba Bal  Hakan Ozkan  Ahmet Adiguzel  Fikrettin Sahin  Derya Yanmis
Institution:1. Ataturk University, Faculty of Science, Department of Biology , Erzurum , Turkey;2. Erzincan University, Faculty of Science and Arts, Department of Biology , Erzincan , Turkey;3. Ataturk University, Faculty of Science, Department of Molecular Biology and Genetics , Erzurum , Turkey;4. Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering , Istanbul , Turkey
Abstract:In this study, the bacteria having ore enrichment potential were isolated from three different magnesite quarries located in Erzurum-Askale borderlines. The obtained isolates were identified and characterized according to the conventional (morphological, physiological and biochemical tests) and molecular techniques (fatty acid methyl ester profiles (FAME), BOX PCR and 16S rDNA). According to sequence analysis, they were determined as Exiguobacterium aurantiacum (4), Exiguobacterium sibiricum (2), Bacillus sp. (2), Staphylococcus epidermidis (2), Staphylococcus haemolyticus (1), Shewanella baltica (1) and Klebsiella oxytoca (1), respectively.
Keywords:bacteria  BOX PCR  FAMEs  isolation  magnesite  16S rDNA
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