全文获取类型
收费全文 | 1281篇 |
免费 | 83篇 |
出版年
2023年 | 9篇 |
2022年 | 3篇 |
2021年 | 37篇 |
2020年 | 16篇 |
2019年 | 17篇 |
2018年 | 38篇 |
2017年 | 29篇 |
2016年 | 35篇 |
2015年 | 72篇 |
2014年 | 66篇 |
2013年 | 93篇 |
2012年 | 112篇 |
2011年 | 98篇 |
2010年 | 59篇 |
2009年 | 59篇 |
2008年 | 74篇 |
2007年 | 91篇 |
2006年 | 71篇 |
2005年 | 58篇 |
2004年 | 63篇 |
2003年 | 42篇 |
2002年 | 42篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 6篇 |
1998年 | 4篇 |
1997年 | 6篇 |
1995年 | 14篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 6篇 |
1988年 | 9篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1983年 | 5篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 3篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 6篇 |
1974年 | 8篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1971年 | 6篇 |
1970年 | 5篇 |
1950年 | 2篇 |
1943年 | 2篇 |
排序方式: 共有1364条查询结果,搜索用时 0 毫秒
1.
2.
The marine priapulid Priapulus caudatus has a voluminous body cavity filled with a blood-like fluid containing erythrocytes and leucocytes (amoebocytes). The hematocrit of animals weighing 0.5–14 gm was 2–10%. The erythrocytes contain a hemerythrin blood pigment. The structure of the coelomocytes was studied by light and electron microscopy. The erythrocytes are nucleated and contain marginal bands, vacuoles and occasionally crystals. The cytoplasm has few organelles. The leucocytes are amoeboid motile cells, the cytoplasm of which contains numerous organelles. The most conspicuous of these are oval particles, probably representing developmental stages of lysosomes. Most of these organelles contain tubules stretching from one pole to another. In the hind part of the animal, certain tissues, primarily the posterior warts contain large numbers of coelomocytes. The histological picture is complicated, showing some resemblance to the lymphoepithelial tissues of vertebrates. 相似文献
3.
4.
Artur Scheib 《Journal of mathematical biology》1989,27(3):369-372
A random competition model is reformulated as an urn model and its behavior is analysed. 相似文献
5.
Ignacy Z. Siemion Artur Pdyczak Jerzy Trojnar Michakl Zimecki Zbigniew Wieczorek 《Peptides》1992,13(6):1233-1237
In connection with our discovery of a strong immunosuppressive activity of cyclolinopeptide A (CLA), we investigated immunosuppressive properties of antamanide and a number of its analogues, including symmetrical antamanide, and compared them with the activities of cyclosporin A and CLA. The peptides were investigated by using plaque forming cell (PFC), graft-versus-host (GvH), delayed type hypersensitivity (DTH), and autologous rosette formation cell (ARFC) tests. Antamanide and symmetrical antamanide exhibit an immunosuppressive activity lower than CLA. Linear antamanide fragments are also active. At higher concentrations of the latter peptides, toxic effects occur. 相似文献
6.
Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus 总被引:1,自引:0,他引:1
Margit Laimer da Câmara Machado Artur da Câmara Machado Veronika Hanzer Hans Weiss Ferdinand Regner Herta Steinkellner Diethard Mattanovich Regina Plail Elisabeth Knapp Birgit Kalthoff Hermann Katinger 《Plant cell reports》1992,11(1):25-29
Summary A system was developed which allows the transfer of foreign genes into apricot cultivars. We report the transformation and regeneration of Prunus armeniaca plants with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene ß-glucuronidase (GUS) and pBinPPVm, carrying the coat protein gene of Plum Pox Virus (PPV). The marker gene GUS was used for optical evaluation of the efficiency of the transformation system. The coat protein gene of PPV was used to introduce coat protein mediated resistance against one of the most important pathogens of stone fruit trees in Europe and the whole Mediterranean area. This is the first report of the successful integration of a viral coat protein gene into a fruit tree species, opening a new perspective on the control of the disease.Abbreviations GUS
ß-glucuronidase
- PPV
Plum Pox Virus
- BA
6-benzylaminopurine
- NPTII
neomycin phosphotransferase II
- CP
coat protein
- CaMV
Cauliflower Mosaic Virus
- P35S 35S
promoter
- MS
Murashige and Skoog
- PCR
polymerase chain reaction
- P/C/I
phenol/chloroform/isoamylalcohol
- RNase
ribonuclease
- dNTP
deoxyribonucleosidetriphosphate
- DMSO
dimethyl sulfoxide 相似文献
7.
8.
9.
Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325° to 333deg; 总被引:2,自引:2,他引:0
P. Glaser F. Kunst M. Arnaud M.-P. Coudart W. Gonzales M.-F. Hullo M. Ionescu B. Lubochinsicy L. Marcelino I. Moszer E. Presecan M. Santana E. Schneider J. Schwelzer A. Vertes G. Rapoport A. Danchin 《Molecular microbiology》1993,10(2):371-384
In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome the DNA region located between gerB (314°) and sacXV (333°) was assigned to the Institut Pasteur. In this paper we describe the cloning and sequencing of a segment of 97 kb of contiguous DNA. Ninety-two open reading frames were predicted to encode putative proteins among which only forty-two were found to display significant similarities to known proteins present in databanks, e.g. amino acid permeases, proteins involved in cell wall or antibiotic biosynthesis, various regulatory proteins, proteins of several dehydrogenase families and enzymes II of the phosphotransferase system involved in sugar transport. Additional experiments led to the identification of the products of new B. subtilis genes, e.g. galactokinase and an operon involved in thiamine biosynthesis. 相似文献
10.