Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325° to 333deg; |
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| Authors: | P Glaser F Kunst M Arnaud M-P Coudart W Gonzales M-F Hullo M Ionescu B Lubochinsicy L Marcelino I Moszer E Presecan M Santana E Schneider J Schwelzer A Vertes G Rapoport A Danchin |
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| Institution: | Unitéde Régulation de l'Expression Génétique;Unitéde Biochimie Microbienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France.;GDR 1029, Centre National de la Recherche Scientifique, 28 rue du Dr Roux, 75724 Paris Cedex 15, France.;Laboratoire de Biochimie Cellulaire et de Biologie Moléculaire, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France. |
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| Abstract: | In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome the DNA region located between gerB (314°) and sacXV (333°) was assigned to the Institut Pasteur. In this paper we describe the cloning and sequencing of a segment of 97 kb of contiguous DNA. Ninety-two open reading frames were predicted to encode putative proteins among which only forty-two were found to display significant similarities to known proteins present in databanks, e.g. amino acid permeases, proteins involved in cell wall or antibiotic biosynthesis, various regulatory proteins, proteins of several dehydrogenase families and enzymes II of the phosphotransferase system involved in sugar transport. Additional experiments led to the identification of the products of new B. subtilis genes, e.g. galactokinase and an operon involved in thiamine biosynthesis. |
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