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Correction to: Enhanced productivity of atropine in cell suspension culture of Hyoscyamus muticus L.
Abdelazeez Walla Mohamed Abdelmaksood Anatolievna Kostyukova Yulia Zavdetovna Khusnetdinova Landysh Damirovna Alekbaeva Gulnaz Abou El-Dis Gamil Rayan Arnoldovna Timofeeva Olga 《In vitro cellular & developmental biology. Plant》2022,58(4):702-702
In Vitro Cellular & Developmental Biology - Plant - 相似文献
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Filipp Filippovich Vasilyev Julia Anatolievna Lopatnikova Sergey Vitalievich Sennikov 《Cytotechnology》2013,65(5):795-802
The biological effects of interleukin (IL)-1 are realized through binding to specific membrane-bound receptors. The efficiency of IL-1 action depends on the number of receptors on the cell. We determined the percentage of cells that express IL-1 receptor type I (IL-1RI) and IL-1 receptor type II (IL-1RII) by flow cytometry using phycoerythrin (PE)-labelled antibodies to the IL-1Rs, and the mean absolute number of membrane-bound IL-1Rs per cell using QuantiBRITE PE calibration beads. We showed that different subpopulations of immunocompetent cells expressed different numbers of molecules of membrane-bound IL-1RI and IL-1RII. We also established that when cells were stimulated with bacterial lipopolysaccharide, there was a significant increase in the number of IL-1RI expressed, and a significant decrease in the mean number of IL-1RII molecules per cell. Determination of the mean number of membrane-bound IL-1R molecules using this protocol enables us to obtain precise and reproducible data that are necessary for full evaluation of expression levels. 相似文献
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Ludmila Michaylovna Lukyanenko Aliasksandra Sergeevna Skarabahatava Ekaterina Ivanovna Slobozhanina Svetlana Anatolievna Kovaliova Maria Letizia Falcioni Giancarlo Falcioni 《Journal of trace elements in medicine and biology》2013,27(2):160-167
Aluminum belongs to a group of potential toxic elements capable of penetrating the human body. In this paper, the effect of aluminum concentrations on red blood cell membranes using different fluorescent probes able to localize in various parts of the phospholipid bilayer (TMA-DPH, laurdan and pyrene) were studied. Our results confirm that human erythrocytes exposed to aluminum undergo physico-chemical modifications at the membrane level. A decrease in fluorescence anisotropy of TMA-DPH and in the polarity of the lipid bilayer with a concomitant shift toward a gel phase was observed, and the pyrene excimerization coefficient (kex) increased.Furthermore, the presence of aluminum induced lipid peroxidation and reduced the activity of erythrocyte antioxidant enzymes (SOD, CAT and GSHPx). Al-induced morphological changes on the erythrocyte membrane surface were monitored using atomic force microscopy. These results provide further information on the target of action of different aluminum amounts. 相似文献
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Abdelazeez Walla Mohamed Abdelmaksood Anatolievna Kostyukova Yulia Zavdetovna Khusnetdinova Landysh Damirovna Alekbaeva Gulnaz Abou El-Dis Gamil Rayan Arnoldovna Timofeeva Olga 《In vitro cellular & developmental biology. Plant》2022,58(4):593-605
In Vitro Cellular & Developmental Biology - Plant - For the first time, suspension cultures produced tropane alkaloids and were obtained from root explants of Hyoscyamus muticus L. It was shown... 相似文献
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