全文获取类型
收费全文 | 6925篇 |
免费 | 1149篇 |
国内免费 | 3669篇 |
出版年
2024年 | 25篇 |
2023年 | 222篇 |
2022年 | 360篇 |
2021年 | 368篇 |
2020年 | 349篇 |
2019年 | 377篇 |
2018年 | 263篇 |
2017年 | 291篇 |
2016年 | 276篇 |
2015年 | 352篇 |
2014年 | 488篇 |
2013年 | 503篇 |
2012年 | 617篇 |
2011年 | 660篇 |
2010年 | 554篇 |
2009年 | 595篇 |
2008年 | 643篇 |
2007年 | 593篇 |
2006年 | 554篇 |
2005年 | 506篇 |
2004年 | 413篇 |
2003年 | 330篇 |
2002年 | 314篇 |
2001年 | 319篇 |
2000年 | 308篇 |
1999年 | 220篇 |
1998年 | 114篇 |
1997年 | 90篇 |
1996年 | 60篇 |
1995年 | 59篇 |
1994年 | 42篇 |
1993年 | 47篇 |
1992年 | 64篇 |
1991年 | 43篇 |
1990年 | 65篇 |
1989年 | 54篇 |
1988年 | 39篇 |
1987年 | 45篇 |
1986年 | 37篇 |
1985年 | 46篇 |
1984年 | 33篇 |
1983年 | 32篇 |
1982年 | 39篇 |
1981年 | 33篇 |
1980年 | 24篇 |
1979年 | 30篇 |
1978年 | 28篇 |
1976年 | 22篇 |
1975年 | 20篇 |
1974年 | 25篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
1.
OBJECTIVE: To assess the relation of smoking and alcohol and coffee consumption to active Helicobacter pylori infection. DESIGN: Cross sectional study of patients attending a general practitioner. Active H pylori infection was measured by the 15C-urea breath test and detailed quantitative information on smoking and on alcohol and coffee consumption was obtained by a standardised self administered questionnaire. SETTING: One general practice in Germany. SUBJECTS: 447 patients aged 15-79 who had not had peptic ulcer disease or treatment for H pylori infection. MAIN OUTCOME MEASURES: Prevalence of H pylori infection according to smoking and alcohol and coffee consumption. RESULTS: Overall prevalence of infection was 21% (94/447). There was no significant relation between smoking and active H pylori infection. Alcohol consumption showed a negative dose-response relation and coffee consumption a positive dose-response relation with active infection. After adjustment for potential confounders, the odds ratios for patients who drank < or = 75 g and > 75 g of ethanol a week compared with non-drinkers were 0.90 (95% confidence interval 0.55 to 1.59) and 0.33 (0.16 to 0.68), respectively (P value for trend 0.005, assuming that 1 litre of beer and 0.51 of wine contain on average 50 g of ethanol in south Germany). Adjusted odds ratios for patients who drank < 3 cups and > or = 3 cups of coffee per day compared with those who did not drink coffee were 1.49 (0.71 to 3.12) and 2.49 (1.23 to 5.03), respectively (P value for trend 0.007). CONCLUSION: These results suggest a protective effect of alcohol consumption against active infection with H pylori and an opposite effect of coffee consumption. 相似文献
2.
3.
4.
5.
6.
An assay for very sensitive antigen detection is described which takes advantage of the self- assembly capabilities of semi-synthetic conjugates of DNA and proteins. The general scheme of this assay is similar to a two-sided (sandwich) enzyme-linked immunoassay (ELISA); however, covalent single-stranded DNA–streptavidin (STV) conjugates, capable of hybridizing to complementary surface-bound DNA oligomers, are utilized for the effective immobilzation of either capture antibodies or antigens, rather than the chemi- or physisorption usually applied in ELISA. Immuno-PCR (IPCR) is employed as a method for signal generation, utilizing oligomeric reagents obtained by self-assembly of STV, biotinylated DNA and antibodies. In three different model systems, detecting human IgG, rabbit IgG or carcinoembryonic antigen, this combination allowed one to increase the sensitivity of the analogous ELISA ~1000-fold. For example, <0.1 amol/µl (15 pg/ml) of rabbit IgG was detectable. The immunoassay can be carried out in a single step by tagging the analyte with both reagents for capture and read-out simultaneously, thereby significantly reducing handling time and costs of analysis. Moreover, as the spatial selectivity of target immobilization is determined by the specificity of DNA base pairing, the assay is particularly suited for miniaturized microfluidics and lab-on-a-chip devices. 相似文献
7.
8.
The ecological significance of toxic nectar 总被引:18,自引:0,他引:18
Lynn S. Adler 《Oikos》2000,91(3):409-420
Although plant-herbivore and plant-pollinator interactions have traditionally been studied separately, many traits are simultaneously under selection by both herbivores and pollinators. For example, secondary compounds commonly associated with herbivore defense have been found in the nectar of many plant species, and many plants produce nectar that is toxic or repellent to some floral visitors. Although secondary compounds in nectar and toxic nectar are geographically and phylogenetically widespread, their ecological significance is poorly understood. Several hypotheses have been proposed for the possible functions of toxic nectar, including encouraging specialist pollinators, deterring nectar robbers, preventing microbial degradation of nectar, and altering pollinator behavior. All of these hypotheses rest on the assumption that the benefits of toxic nectar must outweigh possible costs; however, to date no study has demonstrated that toxic nectar provides fitness benefits for any plant. Therefore, in addition to these adaptive hypotheses, we should also consider the hypothesis that toxic nectar provides no benefits or is tolerably detrimental to plants, and occurs due to previous selection pressures or pleiotropic constraints. For example, secondary compounds may be transported into nectar as a consequence of their presence in phloem, rather than due to direct selection for toxic nectar. Experimental approaches are necessary to understand the role of toxic nectar in plant-animal interactions. 相似文献
9.
10.