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1.
Intracellular Development of Enterocytozoon, A Unique Microsporidian Found in the Intestine of AIDS Patients 总被引:2,自引:0,他引:2
Enterocytozoon was 1st described in 1985, in an AIDS patient with intestinal malabsorption and diarrhea. Since then, additional cases of infection with this organism have been observed, but only in individuals with AIDS and malabsorption. Intestinal tissue biopsies were obtained from a 45-year-old man prior to AIDS diagnosis, again nine months later and then at autopsy two months later. When the biopsies were examined electron microscopically, both sets contained the microsporidian parasite. However, the 2nd intestinal biopsy, when wasting was much more severe, contained infection in almost every small intestinal enterocyte examined. The parasite was actively developing, allowing us to detail its life cycle. The parasite is apansporoblastic, polysporous and has characteristics not previously reported in the Microsporida: (1) an electron lucent inclusion not usually seen in Microsporida is prominent and always present; (2) extremely elongated sausage-shaped nuclei occur in the proliferative phase of parasite development; (3) the polar tube development uniquely involves the production of electron dense discs, yet results in the formation of a typical spore; and (4) polar tube development occurs prior to the final division of the multi-nucleate sporont. On the basis of these characteristics, we are placing this genus in a new family, Enterocytozoonidae, n. fam. 相似文献
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Quantitative anatomical and other measurements were made onfully expanded flag leaves of a series of diploid, tetraploidand hexaploid Triticum and Aegilops species, and photosyntheticrates per unit leaf area were measured at light saturation (Pmax). Diploids had the highest Pmax, hexaploids the lowest with tetraploidsbeing intermediate. The anatomical features of tetraploids andhexaploids were generally similar, but different from the diploids.The diploids had thinner leaves with less dry matter and chlorophyllper unit area. The surface area of the mesophyll cells per unitvolume of mesophyll tissue was similar for all ploidy levels,as was the ratio mesophyil cell surface area per unit leaf area.It is argued that while these anatomical features are unlikelyto account for the observed variation in Pmax, it is possiblethat other structural factors with which they are correlatedmay causally influence Pmax. One such feature is the averagediffusion path length from the plasmalemma at the cell surfaceto the sites of carboxylation. Anatomy, photosynthesis, mesophyll, cell size, Triticum, Aegilops, polyploidy 相似文献
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ABSTRACT. The circadian locomotor (walking) rhythms of individual forager honeybees ( Apis mellifera ligustica L.) were measured under a variety of conditions. In constant dark the rhythms exhibited endogenous periodicities that were less than 24 h, whereas under constant light the periods tended to be greater than 24 h. Individual honeybees readily entrained to photoperiods, displaying a diurnal pattern of entrainment with most of the activity occurring in late photophase. Evidence is presented which suggests that foraging behaviour and general locomotor behaviour may be governed by two different circadian clock systems. 相似文献
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A thermoplate system is described which enables uniformly illuminatedpetri dish cultures to be maintained under a range of temperatureregimes. The system is evaluated and used to investigate theeffects of incubation temperatures between 10 and 35 ?C on rootinitiation. Sinapis cotyledons root most rapidly and in greatest numbersat 25 and 30 ?C. Below 25 ?C, rooting is reduced and delayed,and above 30 ?C rooting is prevented. The optimum temperaturefor initiation of primordia is close to 25 ?C, where, after4 days over 80 per cent of petioles have at least one primordiumand over half of these are already elongating. Initiation isconsiderably reduced at temperatures above 30 ?C and below 25?C and primordium elongation either fails to take place (35?C) or is delayed (20, 13, 10 ?C). At 25 ?C, 90 per cent of the primordia are initiated in theterminal 1.5 mm of the petriole. A similar pattern occurs atother temperatures, but the relative numbers of primordia initiatedin the upper petiole are increased when cotyledons are incubatedat temperatures below 25 ?C. At 25 ?C a higher proportion ofprimordia are initiated in the upper petiole in cotyledons withmany primordia than in cotyledons with few, but the developmentof primordia to the elongation stage is not affected by theirposition in the petiole nor by the numbers of primordia present. 相似文献
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JEFFREY N. CLARK ANN C. MARCHOK 《Differentiation; research in biological diversity》1979,14(1-3):175-183
Rat tracheal explants maintained as organ cultures exhibited a normal mucociliary epithelium for at least 46 days in the presence of retinyl acetate. In the absence of vitamin A the explant epithelium became quiescent or underwent a metaplastic change to a keratinizing squamous epithelium. This process was accelerated if explants were derived from vitamin A-deficient animals. Autoradiographic examination showed that [3 H]glucosamine label accumulated in various cell types in the explant, but especially in the epithelium. It was found that the explants secreted mucous glycoproteins into the medium and that the production and biochemical characteristics of a specific mucin fraction were dependent upon the vitamin A status of the explant. 相似文献
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Evaluation of tolerance to Pierce's disease and Botrytis in transgenic plants of Vitis vinifera L. expressing the pear PGIP gene 总被引:1,自引:0,他引:1