水稻逆境响应基因OsMsr15的表达分析与克隆

应用Affymetrix水稻表达芯片,分析了苗期和孕穗期的培矮64S水稻基因在高温、干旱和低温逆境处理条件下的表达模式,发现多个与逆境反应相关基因.其中,OsMsr15(Oryza sativa L.multiple stresses responsivegene 15)受高温、干旱和低温胁迫诱导,表达水平均显著上调.实时定量PCR分析结果与芯片数据基本吻合.序列分析表明,该基因无内含子,ORF(open reading frame)全长为717 bp,编码一个由238个氨基酸残基组成、具有典型C2H2结构域的锌指蛋白,推测相对分子质量约为2.46 kD,pI值约为8.90,蛋白分子的氨基端和羧基端分别存在一个推测的核定位信号B-box和EAR(ERF-associated amphiphilic repression)基序.对OsMsr15可能的启动子区域进行分析,发现多种与逆境诱导相关的调控元件.该基因编码的蛋白在不同物种中存在高度相似性,显示OsMsr15基因可能作为一个保守的耐逆基因,参与植物的多种耐逆反应过程.     

青稞的组织培养与植株再生

1 植物名称。青稞(Hordeum vulgare),又称裸大麦,有些地方称为元麦、米麦。6个青稞品种均由西藏农牧科学院青稞研究与发展中心提供。     

玉米淀粉生物合成及其遗传操纵

淀粉是许多植物重要的储藏物质。淀粉突变体以及转基因植物中淀粉变异的特点使我们对淀粉生物合成的过程有了较深入的了解,许多研究的结果揭示了玉米淀粉的生物合成涉及4类酶--ADPG焦磷酸化酶、淀粉合成酶、淀粉分支酶和去分支酶。随着编码这些酶的基因的克隆,利用转基因技术对淀粉合成过程进行遗传操纵业已成为可能,并且在提高淀粉产量以及不同特性淀粉品质的种质资源创新等方面展示出巨大的潜力。 Abstract:Starch is the most important source of calories and a vital storage component in plants.The characterization and production of starch variants from mutation and with transgenic technology has improved our understanding of the synthesis of starch granule.In starch biosynthesis in plants,four enzymes,including ADP-glucose pyrophosphorylase,starch synthase,starch branching enzyme and starch debranching enzyme,are widely accepted from an enormous amount of research aimed primarily at enzyme characterization.As many genes encoding the enzymes and their multiple isoforms in starch biosynthesis pathway have been isolated,genetic manipulation of the starch biosynthesis pathway shows to be a practical way by which starch quantity is increased and starch with novel properties can be created.